Spa Radioactive Binding Assay

Description: The Cereblon Binding Assay Kit (FP) is designed for testing and profiling of Cereblon (CRBN) inhibitors using Fluorescence Polarization. The assay is a competitive binding assay, based on the binding of fluorescently-labeled Thalidomide, a CRBN binder, to purified recombinant CRBN._x000D_ CRBN is the substrate recognition component of a DCX (DDB1-CUL4-X-box) E3 protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins. CRBN is a direct protein target for the immunomodulatory and antiproliferative activities of thalidomide analogues such as lenalidomide and pomalidomide._x000D_ The Cereblon Binding Assay Kit (FP) comes in a convenient 96-well format, with enough purified CRBN, Cy5-labeled Thalidomide, and assay buffer for 100 enzyme reactions. In addition, the kit includes the CRBN inhibitor Pomalidomide for use as an inhibitor control._x000D_
The key to the CRBN Assay Kit is the fluorescently-labeled Thalidomide. Using this kit, only one simple step on a microtiter plate is required for CRBN reactions. The Cy5-labeled Thalidomide is incubated with a sample containing CRBN to produce a change in fluorescent polarization that can be measured using a fluorescence reader.

Description: The PCSK9 (D374Y) [Biotinylated]-LDLR Binding Assay Kit is designed for screening and profiling purposes. The kit comes in a convenient 96-well format and contains enough biotin-labeled mutant protein PCSK9 (D374Y), purified LDLR ectodomain, and HRP-conjugated streptavidin for 100 reactions. Moreover, two pre-formulated assay buffers are supplied to validate PCSK9-LDLR binding in either neutral or acidic binding conditions.This assay takes advantage of the high sensitivity of detection of biotin-labeled PCSK9 (D374Y) by streptavidin-HRP. First, a 96-well plate is coated with LDLR ectodomain. PCSK9 (D374Y) is then incubated with LDLR. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which is measured using a chemiluminescence reader.Need us to run inhibitor screens or profile your compounds against PCSK9 (D374Y) -LDLR? Check out ourPCSK9 Screening Services.

Description: This product includes 150 µl of 10 x Buffer, 150 µl of 10 x ssDNA (a 45-mer single-strand DNA) and 100 µl of 4 x gel loading buffer.

Description: Cell Biolabs? HIF-1 Alpha DNA Binding Activity Assay Kit is an immunoassay developed for rapid detection of activated HIF-1 in any protein sample. Active HIF-1 complex is captured on a double stranded oligonucleotide containing an HRE that is attached to the well. HIF-1 Alpha is then detected with an anti-HIF-1 Alpha antibody followed by an HRP conjugated secondary antibody. Each kit provides sufficient reagents to perform up to a total of 96 assays, and can detect HIF-1 Alpha from human, mouse, or rat. The HIF-1 DNA Binding Activity Assay takes only 4.5 hours to complete compared to a standard 3 day electromobility shift (EMSA) method.

Description: This product includes 150 µl of 10 x Buffer, 150 µl of 10 x ssDNA, 150 µl of 2 mg/ml E. coli single-strand DNA binding protein SSB and 100 µl of 4 x gel loading buffer.

Description: The MLL4 (KMT2B) : WDR5 Binding Chemiluminescent Assay Kit is an ELISA-based assay designed to measure the binding between MLL4 (mixed lineage leukemia protein-4, also known as KMT2B) and WDR5 (WD40 repeat-containing protein 5) for screening and profiling applications. The MLL4 (KMT2B) : WDR5 Binding Chemiluminescent Assay comes with enough purified MLL4 (amino acids 2490-2715) and WDR5 proteins, primary and secondary antibody, assay buffer, and detection reagent for 100 enzyme reactions.Figure 1. MLL4 (KMT2B) : WDR5 Binding Chemiluminescent Assay Kit schematic.A 96-well plate is coated with MLL4 protein. After coating and blocking, WDR5 is added in an optimized assay buffer. Next, unbound WDR5 is washed away, and the plate is incubated with a primary antibody followed by a secondary HRP-conjugated antibody. Finally, ELISA ECL substrate is added to produce chemiluminescence that can be measured using a chemiluminescence reader. The chemiluminescence signal is proportional to the efficacy of WDR5 binding to MLL4 (KMT2B) .Need us to run inhibitor screens or profile your compounds against MLL4 (KMT2B) : WDR5 binding? Check out ourMethyltransferase Screening and Profiling.

Description: eEF1A2 binding protein Rabbit Polyclonal Antibody

Description: eEF1A2 binding protein Rabbit Polyclonal Antibody