Akr-190 Cell Biolabs

5X RIPA Buffer with Protease Inhibitor Cocktail

AKR-190 20mL
EUR 160
Description: RIPA Buffer extracts proteins from cultured mammalian cells for various downstream applications.

Cell Biolabs Laboratories manufactures the akr-190 cell biolabs reagents distributed by Genprice. The Akr-190 Cell Biolabs reagent is RUO (Research Use Only) to test human serum or cell culture lab samples. To purchase these products, for the MSDS, Data Sheet, protocol, storage conditions/temperature or for the concentration, please contact Cell BioLabs. Other Akr-190 products are available in stock. Specificity: Akr-190 Category: Cell Group: Biolabs

pMXs Retroviral Vector

10 µg
EUR 624
Description: Use this construct to clone your gene for downstream recombinant retroviral packaging

pMXs-Neo Retroviral Vector

10 µg
EUR 624
Description: Use this construct to clone your gene for downstream recombinant retroviral packaging

pMXs-Puro Retroviral Vector

10 µg
EUR 624
Description: Use this construct to clone your gene for downstream recombinant retroviral packaging

pMXs-IRES-GFP Retroviral Vector

10 µg
EUR 624
Description: Use this construct to clone your gene for downstream recombinant retroviral packaging

pMXs-IRES-Puro Retroviral Vector

10 µg
EUR 624
Description: Use this construct to clone your gene for downstream recombinant retroviral packaging

pMXs-IRES-Neo Retroviral Vector

10 µg
EUR 624
Description: Use this construct to clone your gene for downstream recombinant retroviral packaging

pMXs-IRES-Bsd Retroviral Vector

10 µg
EUR 624
Description: Use this construct to clone your gene for downstream recombinant retroviral packaging

Biolabs information

Mouse Anti-RFP Monoclonal Antibody

AKR-021 100 µg
EUR 508
Description: Mouse Anti-RFP Monoclonal Antibody recognizes Tag-RFP, Turbo-RFP, mCherry and mOrange. Suitable for Western Blot, Dot Blot, Immunostaining or ELISA. Clone RF5R. 100 µg.

?-Galactosidase Staining Kit

AKR-100 1 kit
EUR 432
Description: This simple assay measures the transfection efficiency of the LacZ gene using a simple staining protocol.

Phospho Antibody Stripping Solution

AKR-102 1 kit
EUR 392
Description: This reagent allows you to strip phospho antibodies from protein blots and subsequently re-probe the same blot.

PhosphoBlocker Blocking Reagent

AKR-103 1L
EUR 328
Description: Most commercially available Western blot blockers, such as dry milk or serum, are sufficient to block unreactive sites on the membrane. However, they are not designed to preserve phosphoprotein antigens during blotting. Our PhosphoBLOCKER Blocking Reagent provides superior blocking by maximizing signal-to-noise ratio. The PhosphoBLOCKER reagent particluarly excels with very low levels of endogenous phopsphoproteins.

PhosphoBlocker Blocking Reagent

AKR-104 4L
EUR 711
Description: Most commercially available Western blot blockers, such as dry milk or serum, are sufficient to block unreactive sites on the membrane. However, they are not designed to preserve phosphoprotein antigens during blotting. Our PhosphoBLOCKER Blocking Reagent provides superior blocking by maximizing signal-to-noise ratio. The PhosphoBLOCKER reagent particluarly excels with very low levels of endogenous phopsphoproteins.

Phosphoprotein Purification Kit

AKR-105 2 preps
EUR 299
Description: The Phosphoprotein Purification Kit provides an efficient system for quick purification/enrichment of phosphoproteins from various samples. Phosphorylated proteins are affinity purified from lysates with a single-step purification matrix. The entire procedure takes about 4 hours. Each preparation can handle 2.5 mg of total lysate protein (approx. one confluent 100 mm dish).

Phosphoprotein Purification Kit

AKR-106 5 preps
EUR 485
Description: The Phosphoprotein Purification Kit provides an efficient system for quick purification/enrichment of phosphoproteins from various samples. Phosphorylated proteins are affinity purified from lysates with a single-step purification matrix. The entire procedure takes about 4 hours. Each preparation can handle 2.5 mg of total lysate protein (approx. one confluent 100 mm dish).

Rapid GST Inclusion Body Solubilization and Renaturation Kit

AKR-110 1 kit
EUR 508
Description: Recombinant proteins expressed in bacteria often form inclusion bodies, especially when they are expressed at high levels. The Rapid GST Inclusion Body Solubilization and Renaturation Kit is designed to retrieve expressed GST fusion proteins in soluble form after lysis and extraction procedures. Each kit contains sufficient reagents to solubilize and renature up to 5-10 liters of bacterial culture.

GFP Quantitation Kit

AKR-120 100 assays
EUR 577
Description: Cell Biolabs? GFP Quantitation Kit measures GFP fluorescence in a fluorometer. The quantity of rGFP in sample is determined by comparing its fluorescence reading with that of known recombinant GFP standard curve. The kit has detection sensitivity limit of 100 ng/mL. A proprietary GFP quench solution is also included for determining autofluorescence of cell or tissue sample. Each kit provides sufficient reagents to perform up to 100 assays including standard curve and GFP samples.

GFP ELISA Kit

AKR-121 96 assays
EUR 734
Description: Most imaging studies of rGFP are qualitative, and quantitation by FACS is time-consuming and expensive. Our GFP ELISA Kit measures green fluorescent protein in a standard microplate reader. Assay is sensitive to 30 pg/mL.

RFP ELISA Kit

AKR-122 96 assays
EUR 734
Description: Most imaging studies of rRFP are qualitative, and quantitation by FACS is time-consuming and expensive. Our RFP ELISA Kit measures RFP fluorescence by ELISA in a standard microplate reader.

His-Tag Protein ELISA Kit

AKR-130 96 wells
EUR 601
Description: Our His-Tag Protein ELISA Kit allows you to detect and quantify His-tagged protein samples simply and reliably by comparing your unknown samples to a known recombinant standard. Sensitivity range of the kit is 4 µg/mL to 1 ng/mL, or 950 nM to 50 pM of 6xHis-tag residues.

Rapid Antibody Purification Kit

AKR-160 10 assays
EUR 519
Description: Cell Biolabs? Rapid Antibody Purification kit is designed for rapid, single-step purification of high-quality IgG from ascites, serum and tissue culture media or hybridoma supernatants.

Nuclear/Cytosolic Fractionation Kit

AKR-171 20 preps
EUR 276
Description: Cell Biolabs? Nuclear/Cytosolic Fractionation Kit provides a simple and fast tool to isolate nuclear extract from the cytoplasmic fraction of mammalian cells.  The procedure has been optimized to provide extraction, with high protein recovery and low cross-contamination, in less than 2 hours.  The extracted protein fractions are functional and suitable for downstream assays such as DNA footprinting, RNA splicing, gel shift assays (EMSA), reporter assays, enzyme activity assays, and Western blotting.

Nuclear/Cytosolic Fractionation Kit

AKR-172 100 preps
EUR 664
Description: Cell Biolabs? Nuclear/Cytosolic Fractionation Kit provides a simple and fast tool to isolate nuclear extract from the cytoplasmic fraction of mammalian cells.  The procedure has been optimized to provide extraction, with high protein recovery and low cross-contamination, in less than 2 hours.  The extracted protein fractions are functional and suitable for downstream assays such as DNA footprinting, RNA splicing, gel shift assays (EMSA), reporter assays, enzyme activity assays, and Western blotting.

5X Bacterial Protein Extraction Reagent (Tris)

AKR-180 50mL
EUR 276
Description: The Cell Biolabs Bacterial Protein Extraction Reagents contain a gentle, nonionic detergent formulation which quickly extracts functional, recombinant protein from E. coli without mechanical disruption.

5X Bacterial Protein Extraction Reagent (Phosphate)

AKR-181 50mL
EUR 276
Description: The Cell Biolabs Bacterial Protein Extraction Reagents contain a gentle, nonionic detergent formulation which quickly extracts functional, recombinant protein from E. coli without mechanical disruption.