Home » Fluorescent Ligand Binding Assay

Fluorescent Ligand Binding Assay

Cereblon Binding Assay Kit
79899 96 rxns.
EUR 600
Description: The Cereblon Binding Assay Kit (FP) is designed for testing and profiling of Cereblon (CRBN) inhibitors using Fluorescence Polarization. The assay is a competitive binding assay, based on the binding of fluorescently-labeled Thalidomide, a CRBN binder, to purified recombinant CRBN._x000D_ CRBN is the substrate recognition component of a DCX (DDB1-CUL4-X-box) E3 protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins. CRBN is a direct protein target for the immunomodulatory and antiproliferative activities of thalidomide analogues such as lenalidomide and pomalidomide._x000D_ The Cereblon Binding Assay Kit (FP) comes in a convenient 96-well format, with enough purified CRBN, Cy5-labeled Thalidomide, and assay buffer for 100 enzyme reactions. In addition, the kit includes the CRBN inhibitor Pomalidomide for use as an inhibitor control._x000D_
The key to the CRBN Assay Kit is the fluorescently-labeled Thalidomide. Using this kit, only one simple step on a microtiter plate is required for CRBN reactions. The Cy5-labeled Thalidomide is incubated with a sample containing CRBN to produce a change in fluorescent polarization that can be measured using a fluorescence reader.

Binding Assay Laboratories manufactures the fluorescent ligand binding assay reagents distributed by Genprice. The Fluorescent Ligand Binding Assay reagent is RUO (Research Use Only) to test human serum or cell culture lab samples. To purchase these products, for the MSDS, Data Sheet, protocol, storage conditions/temperature or for the concentration, please contact binding assay. Other Fluorescent products are available in stock. Specificity: Fluorescent Category: Ligand Group: Binding Assay

HDL and LDL/VLDL Assay Kit
100 assays
EUR 898

PCSK9[Biotinylated]-LDLR Binding Assay Kit
96 rxns.
EUR 805
Description: The PCSK9[Biotinylated]-LDLR Binding Assay Kit is designed for screening and profiling purposes. The key to this kit is the high sensitivity of detection of biotin-labeled PCSK9 by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, LDLR ectodomain is coated on a 96-well plate. Next, PCSK9 is incubated with LDLR on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can then be measured using a chemiluminescence reader.

Mouse Chemokine Ligand 2 (CCL2) Detecton Assay Kit
1 kit
EUR 580.26
Description: Mouse Chemokine Ligand 2 (CCL2) Detecton Assay Kit

Mouse Chemokine Ligand 5 (CCL5) Detection Assay Kit
1 kit
EUR 580.26
Description: Mouse Chemokine Ligand 5 (CCL5) Detection Assay Kit

Mouse Chemokine Ligand 25 (CCL25) Detection Assay Kit
1 kit
EUR 580.26
Description: Mouse Chemokine Ligand 25 (CCL25) Detection Assay Kit

Total Iron-Binding Capacity (TIBC) and Serum Iron Assay Kit (Colorimetric)
each
EUR 698.4

XTT CELL VIABILITY ASSAY KIT (1000 ASSAY)
1KIT
EUR 283.2
Description: Minimum order quantity: 1 unit of 1KIT

Binding Assay information

Cell Meter™ Live Cell Caspase 3/7 Binding Assay Kit *Green Fluorescence*
20100 25 Tests
EUR 334

Cell Meter™ Annexin V Binding Apoptosis Assay Kit *Blue Fluorescence Excited at 405 nm*
22828 100 Tests
EUR 222

Cell Meterâ„¢ Annexin V Binding Apoptosis Assay Kit *Blue Fluorescence Excited at 405 nm*
22828-100Tests 100 Tests
EUR 222
Description: Our Cell Meterâ„¢ assay kits are a set of tools for monitoring cell viability.

Cell Meterâ„¢ Annexin V Binding Apoptosis Assay Kit *Green Fluorescence Excited at 405 nm*
22829-100Tests 100 Tests
EUR 222
Description: Our Cell Meterâ„¢ assay kits are a set of tools for monitoring cell viability.

Cell Meter™ Annexin V Binding Apoptosis Assay Kit *Green Fluorescence Excited at 405 nm*
22829 100 Tests
EUR 222

Cell Meter™ Annexin V Binding Apoptosis Assay Kit *Orange Fluorescence Excited at 405 nm*
22830 100 Tests
EUR 222

Cell Meterâ„¢ Annexin V Binding Apoptosis Assay Kit *Orange Fluorescence Excited at 405 nm*
22830-100Tests 100 Tests
EUR 222
Description: Our Cell Meter™ assay kits are a set of tools for monitoring cell viability.

Cell Meterâ„¢ Generic Fluorimetric Caspase Binding Assay Kit *Red Fluorescence Optimized for Flow Cytometry*
22822-100Tests 100 Tests
EUR 222
Description: Our Cell Meter™ assay kits are a set of tools for monitoring cell viability.

Cell Meter™ Generic Fluorimetric Caspase Binding Assay Kit *Red Fluorescence Optimized for Flow Cytometry*
22822 100 Tests
EUR 222

Cell Meterâ„¢ Annexin V Binding Apoptosis Assay Kit *Red Fluorescence Optimized for Flow Cytometry*
22826-100Tests 100 Tests
EUR 222
Description: Our Cell Meter™ assay kits are a set of tools for monitoring cell viability.

Cell Meter™ Annexin V Binding Apoptosis Assay Kit *Red Fluorescence Optimized for Flow Cytometry*
22826 100 Tests
EUR 222

Cell Meterâ„¢ Annexin V Binding Apoptosis Assay Kit *Green Fluorescence Optimized for Flow Cytometry*
22824-100Tests 100 Tests
EUR 222
Description: Our Cell Meter™ assay kits are a set of tools for monitoring cell viability.

Cell Meterâ„¢ Annexin V Binding Apoptosis Assay Kit *Orange Fluorescence Optimized for Flow Cytometry*
22825-100Tests 100 Tests
EUR 222
Description: Our Cell Meter™ assay kits are a set of tools for monitoring cell viability.

Cell Meter™ Annexin V Binding Apoptosis Assay Kit *Green Fluorescence Optimized for Flow Cytometry*
22824 100 Tests
EUR 222

Cell Meter™ Annexin V Binding Apoptosis Assay Kit *Orange Fluorescence Optimized for Flow Cytometry*
22825 100 Tests
EUR 222

Cell Meterâ„¢ Annexin V Binding Apoptosis Assay Kit *Deep Red Fluorescence Optimized for Flow Cytometry*
22827-100Tests 100 Tests
EUR 222
Description: Our Cell Meter™ assay kits are a set of tools for monitoring cell viability.

Cell Meter™ Annexin V Binding Apoptosis Assay Kit *Deep Red Fluorescence Optimized for Flow Cytometry*
22827 100 Tests
EUR 222