Klhl12 Disheveled Binding Assay

Description: Mouse Monoclonal to KLHL12

Description: Antibody raised against KLHL12

Description: Mouse monoclonal to KLHL12.

Description: This gene encodes a member of the KLHL (Kelch-like) family of proteins. This protein has been identified as an autoantigen in the autoimmune disease Sjogren's syndrome and as a potential biomarker in primary biliary cirrhosis. This protein may act as a substrate adaptor of the Cullin-3 ubiquitin ligase complex to promote substrate-specific ubiquitylation. Ubiquitylation by this complex has been shown to regulate the Wnt signaling pathway as well as COPII vesicle coat size. A pseudogene has been identified on chromosome 22. Alternative splicing results in multiple transcript variants.

Description: The Cereblon Binding Assay Kit (FP) is designed for testing and profiling of Cereblon (CRBN) inhibitors using Fluorescence Polarization. The assay is a competitive binding assay, based on the binding of fluorescently-labeled Thalidomide, a CRBN binder, to purified recombinant CRBN._x000D_ CRBN is the substrate recognition component of a DCX (DDB1-CUL4-X-box) E3 protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins. CRBN is a direct protein target for the immunomodulatory and antiproliferative activities of thalidomide analogues such as lenalidomide and pomalidomide._x000D_ The Cereblon Binding Assay Kit (FP) comes in a convenient 96-well format, with enough purified CRBN, Cy5-labeled Thalidomide, and assay buffer for 100 enzyme reactions. In addition, the kit includes the CRBN inhibitor Pomalidomide for use as an inhibitor control._x000D_
The key to the CRBN Assay Kit is the fluorescently-labeled Thalidomide. Using this kit, only one simple step on a microtiter plate is required for CRBN reactions. The Cy5-labeled Thalidomide is incubated with a sample containing CRBN to produce a change in fluorescent polarization that can be measured using a fluorescence reader.

Description: The EZH2-EED Binding Assay Kit is designed to measure the inhibition of the_x000D_protein-protein interaction between EZH2 and EED. The key to this kit is the specific binding of EZH2 to EED. With this kit, only two_x000D_simple steps on a microtiter plate are required. First, incubate a sample containing EZH2, EED_x000D_and an inhibitor of choice. Next, incubate with a mixture of acceptor and donor beads, followed_x000D_by reading the Alpha-counts.

Description: A polyclonal antibody against KLHL12. Recognizes KLHL12 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA

Description: A polyclonal antibody against KLHL12. Recognizes KLHL12 from Human. This antibody is FITC conjugated. Tested in the following application: ELISA

Description: A polyclonal antibody against KLHL12. Recognizes KLHL12 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA