Factor Viii Binding Assay

Description: The Cereblon Binding Assay Kit (FP) is designed for testing and profiling of Cereblon (CRBN) inhibitors using Fluorescence Polarization. The assay is a competitive binding assay, based on the binding of fluorescently-labeled Thalidomide, a CRBN binder, to purified recombinant CRBN._x000D_ CRBN is the substrate recognition component of a DCX (DDB1-CUL4-X-box) E3 protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins. CRBN is a direct protein target for the immunomodulatory and antiproliferative activities of thalidomide analogues such as lenalidomide and pomalidomide._x000D_ The Cereblon Binding Assay Kit (FP) comes in a convenient 96-well format, with enough purified CRBN, Cy5-labeled Thalidomide, and assay buffer for 100 enzyme reactions. In addition, the kit includes the CRBN inhibitor Pomalidomide for use as an inhibitor control._x000D_
The key to the CRBN Assay Kit is the fluorescently-labeled Thalidomide. Using this kit, only one simple step on a microtiter plate is required for CRBN reactions. The Cy5-labeled Thalidomide is incubated with a sample containing CRBN to produce a change in fluorescent polarization that can be measured using a fluorescence reader.

Description: Minimum order quantity: 1 unit of 1KIT

Description: Minimum order quantity: 1 unit of 1KIT

Description: Purified Polyclonal Factor VIII antibody

Description: Coagulation Factor VIII (FVIII) is an essential blood-clotting protein. By in situ hybridization, Tantravahi et al.(1986) concluded that the F8 gene is located in the proximal part of chromosome Xq28 with probes DX13 and St14 distally located. The F8 gene encodes coagulation Factor VIII, a large plasma glycoprotein that functions in the blood coagulation cascade as a cofactor for the F9a-dependent activation of F10. Factor VIII is activated proteolytically by a variety of coagulation enzymes, including thrombin(F2). It is tightly associated in the blood with VWF, which serves as a protective carrier protein for Factor VIII (Toole et al., 1984; Hoyer, 1994).

Description: This product includes 150 µl of 10 x Buffer, 150 µl of 10 x ssDNA (a 45-mer single-strand DNA) and 100 µl of 4 x gel loading buffer.

Description: Cell Biolabs? HIF-1 Alpha DNA Binding Activity Assay Kit is an immunoassay developed for rapid detection of activated HIF-1 in any protein sample. Active HIF-1 complex is captured on a double stranded oligonucleotide containing an HRE that is attached to the well. HIF-1 Alpha is then detected with an anti-HIF-1 Alpha antibody followed by an HRP conjugated secondary antibody. Each kit provides sufficient reagents to perform up to a total of 96 assays, and can detect HIF-1 Alpha from human, mouse, or rat. The HIF-1 DNA Binding Activity Assay takes only 4.5 hours to complete compared to a standard 3 day electromobility shift (EMSA) method.

Description: This product includes 150 µl of 10 x Buffer, 150 µl of 10 x ssDNA, 150 µl of 2 mg/ml E. coli single-strand DNA binding protein SSB and 100 µl of 4 x gel loading buffer.

Description: Sheep polyclonal Factor VIII antibody (HRP) conjugated

Description: Sheep polyclonal Factor VIII antibody (HRP) conjugated

Description: Sheep polyclonal Factor VIII antibody (FITC) conjugated