Mouse Cereblon Binding Assay

Cereblon Binding Assay Kit

79899 96 rxns.
EUR 600
Description: The Cereblon Binding Assay Kit (FP) is designed for testing and profiling of Cereblon (CRBN) inhibitors using Fluorescence Polarization. The assay is a competitive binding assay, based on the binding of fluorescently-labeled Thalidomide, a CRBN binder, to purified recombinant CRBN._x000D_ CRBN is the substrate recognition component of a DCX (DDB1-CUL4-X-box) E3 protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins. CRBN is a direct protein target for the immunomodulatory and antiproliferative activities of thalidomide analogues such as lenalidomide and pomalidomide._x000D_ The Cereblon Binding Assay Kit (FP) comes in a convenient 96-well format, with enough purified CRBN, Cy5-labeled Thalidomide, and assay buffer for 100 enzyme reactions. In addition, the kit includes the CRBN inhibitor Pomalidomide for use as an inhibitor control._x000D_
The key to the CRBN Assay Kit is the fluorescently-labeled Thalidomide. Using this kit, only one simple step on a microtiter plate is required for CRBN reactions. The Cy5-labeled Thalidomide is incubated with a sample containing CRBN to produce a change in fluorescent polarization that can be measured using a fluorescence reader.

Binding Assay Laboratories manufactures the mouse cereblon binding assay reagents distributed by Genprice. The Mouse Cereblon Binding Assay reagent is RUO (Research Use Only) to test human serum or cell culture lab samples. To purchase these products, for the MSDS, Data Sheet, protocol, storage conditions/temperature or for the concentration, please contact binding assay. Other Mouse products are available in stock. Specificity: Mouse Category: Cereblon Group: Binding Assay

Ring1 And YY1 Binding Protein (RYBP) Magnetic Luminex Assay Kit

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1KIT
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CytoSelect Cell Viability and Cytotoxicity Assay

96 assays
EUR 470.4
Description: The CytoSelect Cell Viability and Cytotoxicity Assay Kit provides a simple format for monitoring cell viability via metabolic activity. Live cells are detected with either MTT (colorimetric detection) or Calcein AM (fluorometric detection). Dead cells are detected by EthD-1 reagent (fluorometric). All 3 detection reagents are included, along with Saponin (a cell death initiator). Prior to the assay, cells may be treated with compounds or agents that affect cell viability. This kit is suitable for eukaryotic cells, not yeast or bacteria.

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Description: This product includes 60 ul of 1000 x PSA dye for more than 200 assays with the assay volume of 0.3 ml using 96-well plates or cuvettes.

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PCSK9[Biotinylated]-LDLR Binding Assay Kit

96 rxns.
EUR 805
Description: The PCSK9[Biotinylated]-LDLR Binding Assay Kit is designed for screening and profiling purposes. The key to this kit is the high sensitivity of detection of biotin-labeled PCSK9 by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, LDLR ectodomain is coated on a 96-well plate. Next, PCSK9 is incubated with LDLR on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can then be measured using a chemiluminescence reader.

Binding Assay information

Mouse Cereblon (CRBN) ELISA Kit

RDR-CRBN-Mu-48Tests 48 Tests
EUR 668.4

Mouse Cereblon (CRBN) ELISA Kit

RDR-CRBN-Mu-96Tests 96 Tests
EUR 928.8

Mouse Cereblon (CRBN) ELISA Kit

RD-CRBN-Mu-48Tests 48 Tests
EUR 639.6

Mouse Cereblon (CRBN) ELISA Kit

RD-CRBN-Mu-96Tests 96 Tests
EUR 888

Mouse Cereblon (CRBN) ELISA Kit

RDR-CRBN-Mu-48T 48T
EUR 487.52
Description: tissue homogenates and other biological fluids.

Mouse Cereblon (CRBN) ELISA Kit

RDR-CRBN-Mu-96T 96T
EUR 696.47
Description: tissue homogenates and other biological fluids.

Mouse Cereblon (CRBN) ELISA Kit

DL-CRBN-Mu 96T
EUR 453
Description: tissue homogenates or other biological fluids.

Mouse Cereblon (CRBN) ELISA Kit

DLR-CRBN-Mu 96T
EUR 475
Description: tissue homogenates or other biological fluids.

Mouse Cereblon (CRBN) ELISA Kit

RD-CRBN-Mu-48T 48T
EUR 464.3
Description: tissue homogenates and other biological fluids.

Mouse Cereblon (CRBN) ELISA Kit

RD-CRBN-Mu-96T 96T
EUR 663.3
Description: tissue homogenates and other biological fluids.

Mouse Cereblon (CRBN) ELISA Kit

EKN44189-48T 48T
EUR 389.62

Mouse Cereblon (CRBN) ELISA Kit

EKN44189-5x96T 5x96T
EUR 2643.85

Mouse Cereblon (CRBN) ELISA Kit

EKN44189-96T 96T
EUR 556.6

Mouse CRBN(Cereblon) ELISA Kit

ELK7246-48T 48T Ask for price
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CRBN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CRBN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CRBN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CRBN in the samples is then determined by comparing the OD of the samples to the standard curve.

Mouse CRBN(Cereblon) ELISA Kit

ELK7246-96T 96T Ask for price
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CRBN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CRBN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CRBN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CRBN in the samples is then determined by comparing the OD of the samples to the standard curve.

ELISA kit for Mouse CRBN (Cereblon)

ELK7246 1 plate of 96 wells
EUR 518.4
Description: A sandwich ELISA kit for detection of Cereblon from Mouse in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

Mouse Protein cereblon, Crbn ELISA KIT

ELI-10495m 96 Tests
EUR 1038