Elisa Assays Bioassay

Description: Minimum order quantity: 1 unit of 1KIT

Description: Minimum order quantity: 1 unit of 1KIT

Description: Minimum order quantity: 1 unit of 1KIT

Description: For quantitative determination of iron ions Fe³⁺ and/or Fe²⁺ and evaluation of drug effects on iron metabolism. Key Features: Sensitive and accurate. Linear detection range 27 µg/dL (4.8 µM) to 1,000 µg/dL (179 µM) iron in 96-well plate assay. Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 40 min. Can be readily automated as a high-throughput assay for thousands of samples per day. Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvette or 96-well plate assay. Low interference in biological samples. No pretreatments are needed. Assays can be directly performed on serum samples. Method: OD590nm. Samples: Biological (e.g. serum) and environmental samples. Species: All. Procedure: Assay takes 30 min. Kit size: 250 tests. Detection limit: 27 µg/dL (4.8 µM).

Description: For quantitative determination of heme and evaluation of drug effects on heme metabolism. Key Features: Sensitive and accurate. Linear detection range 0.6 - 125 µM heme in 96-well plate assay. Simple and high-throughput. The "mix-and-read" procedure involves addition of a single working reagent and reading the optical density. Can be readily automated as a high-throughput assay in 96-well plates for thousands of samples per day. Safety. Reagents are non-toxic. Versatility. Assays can be executed in 96-well plate or cuvet. Method: OD400nm. Samples: Blood, serum, plasma, urine etc. Species: All. Procedure: Assay takes 5 min. Kit size: 250 tests. Detection limit: 0.6 µM.

Description: For quantitative determination of zinc ion Zn²⁺ and evaluation of drug effects on zinc metabolism. Key Features: Sensitive and accurate. Uses 50 µL samples. Linear detection range 0.12 µM (0.78 µg/dL) to 10 µM (65 µg/dL) zinc in 96-well assay format. Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 30 min. Can be readily automated as a high-throughput assay for thousands of samples per day. Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvette or 96-well plate assay formats possible. Low interference in biological samples. No pretreatments are needed. Method: OD425nm. Samples: Serum, plasma, urine, saliva, food, beverage and environment. Species: All. Procedure: Assay takes 30 min. Kit size: 250 tests. Detection limit: 0.78 µg/dL (0.12 µM).

Description: For quantitative enzymatic determination of urea. Key Features: Fast and sensitive. Linear detection range (20 µL sample): 1 mg/mL (0.17 mM) to 100 mg/mL (17 mM) urea in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the absorbance after 5 minutes. Room temperature assay. No 37°C heater is needed. High-throughput. Homogeneous "mix-incubate-measure" type assay.Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: OD557nm. Samples: Urea in biological samples (e.g. plasma, serum, urine) and food/beverage samples (e.g. milk). Species: All. Procedure: Assay takes 5 min. Kit size: 100 tests. Detection limit: 1 mg/mL (0.17 mM).

Description: For quantitative determination of L-malate (L-malic acid) and drug effects on malate metabolism. Key Features: Fast and sensitive. Use of 20 µL sample. Linear detection range 0.02 to 2 mM L-malate in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the optical density at time 15 minutes. Room temperature assay. No 37°C heater is needed. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: OD565nm. Samples: Malate in food, juice, beverage and other agricultural products. Species: All. Procedure: Assay takes 15 min. Kit size: 100 tests. Detection limit: 0.02 mM.

Description: For sensitive and high-throughput phosphate determination. Key Features: Reagent very stable. Due to our innovative formulation, no precipitation of reagent occurs. Therefore no filtration of reagent is needed prior to assays, as is often required with other commercial kits. High sensitivity and wide detection range: detection of as little of 20 pmoles of phosphate and useful range between 0.4 µM and 50 µM phosphate. Fast and convenient: single reagent "mix-and-measure" assay allows quantitation of free phosphate within 30 minutes. Compatible with routine laboratory and HTS formats: assays can be performed in tubes, cuvettes or microplates, on spectrophotometers and plate readers. Robust and amenable to HTS: Z factors of 0.7 to 0.9 are observed in 96-well plates. Can be readily automated on HTS liquid handling systems Method: OD620nm (malachite green). Samples: Enzyme reactions with free phosphate releases. Species: All. Procedure: Assay takes 30 min. Kit size: 500 tests. Detection limit: 0.4 µM.

Description: Quantitative determination of phosphatase activity by colorimetric (405nm) method. Kit size: 1000 tests. Shelf life: 12 months. Shipping: ambient temp; storage: 4, -20°C.

Description: For quantitative determination of phosphatase enzyme activity. Key Features: High sensitivity and wide linear range. The detection limit is generally 3 ng phosphatase or below. Homogeneous and simple procedure. No wash or reagent transfer steps are involved. The assay can be completed within 30 minutes. Robust and amenable to HTS. All reagents are compatible with high-throughput liquid handling instruments. Method: OD405nm. Samples: Protein phosphatases. Species: All. Procedure: Assay takes 30 min. Kit size: 500 tests. Detection limit: 3 ng.

Description: Rapid homogenous assay for protein kinase activity and high-throughput screen for kinase inhibitors. Key Features: Safe. Non-radioactive assay. Sensitive and accurate. As low as 0.01 U/L kinase can be quantified. Homogeneous and convenient. "Mix-incubate-measure" type assay. The whole assay involves adding a single working reagent and incubation for 10 min at room temperature. Robust and amenable to HTS: Assay can tolerate up to 300 µM ATP and 10% dimethylsulfoxide (DMSO). Z factors of > 0.6 are routinely observed in 96/384-well plates. Can be readily automated on HTS liquid handling systems for tens of thousands of assays per day. Method: FL530/585nm. Samples: All kinases. Species: All. Procedure: Assay takes 10 min. Kit size: 400 tests. Detection limit: 0.01 U/L.

Description: For quantitative determination of NADP and NADPH and evaluation of drug effects on their metabolism (This product is a replacement for the discontinued product EFNP-100). Key Features: Sensitive and accurate. Detection limit of 0.01 µM and linearity up to 1 µM NADP+/NADPH in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the fluorescence at time zero and 30 min. Room temperature assay. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: FL530/585nm. Samples: Cell, tissue extracts etc. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 0.01 µM.

Description: Sensitive determination of NADP and NADPH by fluorometric (530/585nm) method. Procedure: 30 min. Kit size: 100 tests. Detection limit: 0.01 µM. Shelf life: 6 months. Shipping: on ice; storage: -20°C.

Description: For quantitative determination of starch and evaluation of drug effects on starch metabolism. Key Features: Use as little as 10 µL samples. Linear detection range: 2 to 200 µg/mL starch for colorimetric assays and 0.2 to 20 µg/mL for fluorimetric assays. Method: OD570nm, or FL530/585nm. Samples: Biological, agriculture, food etc. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 2 µg/mL.

Description: For quantitative determination of boron in agricultural and environmental samples. Key Features: Fast and sensitive. Linear detection range: 0.05 to 10 µg/mL (0.05 - 10 ppm) boron with 100 µL sample (96-well). Convenient. The procedure involves adding a single working reagent. High-throughput. "Add-mix-read" type assay. Can be readily automated as a high-throughput 96-well or 384-well plate assay for thousands of samples per day. Method: OD420nm. Samples: Water, plant tisse, soil samples, and antibody conjugation solutions. Species: All. Procedure: Assay takes 40 min. Kit size: 100 tests. Detection limit: 0.05 µg/mL or 0.05 ppm.

Description: For quantitative determination of urea and evaluation of drug effects on urea metabolism. Key Features: Sensitive and accurate. Use 5 µL samples. Linear detection range 0.08 mg/dL (13 µM) to 100 mg/dL (17 mM) urea in 96-well plate assay. Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 20 min. Can be readily automated as a high-throughput assay for thousands of samples per day. Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvet or 96-well plate assay. Low interference in biological samples. No pretreatments are needed. Assays can be directly performed on raw biological samples i.e., in the presence of lipid and protein. Method: OD520nm (Chemical). Samples: Serum, plasma, urine, milk, cell/tissue culture, bronchoalveolar lavage (BAL), food, beverage and environment. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 0.08 mg/dL (13 µM).

Description: For quantitative determination of lipid peroxides (thiobarbituric acid reactive substances, TBARS) and evaluation of drug effects on lipid peroxidation. Key Features: Sensitive and accurate. Linear detection range: colorimetric assay 1 - 30 µM, fluorometric assay 0.1 - 1.5 µM MDA. Method: OD535nm, or FL530/550nm. Samples: Serum, plasma, urine, saliva etc. Species: All. Procedure: Assay takes 80 min. Kit size: 100 tests. Detection limit: 0.1 µM.

Description: For quantitative determination of ammonia/ammonium and evaluation of drug effects on their metabolism. Key Features: High sensitivity and wide linear range. Use 20 µL sample. Linear detection range 24 to 1000 µM ammonia. Homogeneous and simple procedure. Simple "mix-and-measure" procedure allows reliable quantitation of ammonia within 30 minutes. Method: OD340nm, or FL360/450nm. Samples: Serum, plasma, urine, saliva, cell culture etc. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 24 µM.

Description: For quantitative determination of acetic acid or acetate and evaluation of drug effects on acetate metabolism. Key Features: Sensitive. Use as little as 10 µL samples. Detection range: 0.20 to 20 mM acetate for colorimetric assays and 0.13 to 2 mM for fluorimetric assays. Simple. The assay involves adding a single working reagent, incubation for 30 min at room temperature and read. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD570nm, or FL530/585nm. Samples: Serum, plasma, food, agriculture and environment. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 0.13 mM.