Purpose Of Binding Assay

Description: The PCSK9[Biotinylated]-LDLR Binding Assay Kit is designed for screening and profiling purposes. The key to this kit is the high sensitivity of detection of biotin-labeled PCSK9 by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, LDLR ectodomain is coated on a 96-well plate. Next, PCSK9 is incubated with LDLR on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can then be measured using a chemiluminescence reader.

Description: The IDO1 Inhibitor Mechanism of Action (MoA) Assay Kit is designed to_x000D_determine the mechanism of IDO1 enzyme inhibition (e.g., reversible or irreversible inhibition)._x000D_The IDO1 Mechanism of Action Assay Kit is simple to use. Inhibitor and_x000D_enzyme are preincubated allowing the inhibitor to bind. After preincubation, the mixture is_x000D_diluted into reaction buffer containing the L-tryptophan (L-Trp) substrate and all necessary_x000D_coupled reaction components. This allows reversible inhibitors to dissociate, while irreversible_x000D_inhibitors remain associated with the IDO1 enzyme. IDO1 activity is determined by measuring_x000D_the absorption of the reaction product at
=320 - 325 nm.