Real Time Binding Assay

Cereblon Binding Assay Kit

79899 96 rxns.
EUR 600
Description: The Cereblon Binding Assay Kit (FP) is designed for testing and profiling of Cereblon (CRBN) inhibitors using Fluorescence Polarization. The assay is a competitive binding assay, based on the binding of fluorescently-labeled Thalidomide, a CRBN binder, to purified recombinant CRBN._x000D_ CRBN is the substrate recognition component of a DCX (DDB1-CUL4-X-box) E3 protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins. CRBN is a direct protein target for the immunomodulatory and antiproliferative activities of thalidomide analogues such as lenalidomide and pomalidomide._x000D_ The Cereblon Binding Assay Kit (FP) comes in a convenient 96-well format, with enough purified CRBN, Cy5-labeled Thalidomide, and assay buffer for 100 enzyme reactions. In addition, the kit includes the CRBN inhibitor Pomalidomide for use as an inhibitor control._x000D_
The key to the CRBN Assay Kit is the fluorescently-labeled Thalidomide. Using this kit, only one simple step on a microtiter plate is required for CRBN reactions. The Cy5-labeled Thalidomide is incubated with a sample containing CRBN to produce a change in fluorescent polarization that can be measured using a fluorescence reader.

Binding Assay Laboratories manufactures the real time binding assay reagents distributed by Genprice. The Real Time Binding Assay reagent is RUO (Research Use Only) to test human serum or cell culture lab samples. To purchase these products, for the MSDS, Data Sheet, protocol, storage conditions/temperature or for the concentration, please contact binding assay. Other Real products are available in stock. Specificity: Real Category: Time Group: Binding Assay

HDL and LDL/VLDL Assay Kit

100 assays
EUR 898

H2O2 and POD Assay Kit (Fluorometric)

100 tests
EUR 145

PCSK9[Biotinylated]-LDLR Binding Assay Kit

96 rxns.
EUR 805
Description: The PCSK9[Biotinylated]-LDLR Binding Assay Kit is designed for screening and profiling purposes. The key to this kit is the high sensitivity of detection of biotin-labeled PCSK9 by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, LDLR ectodomain is coated on a 96-well plate. Next, PCSK9 is incubated with LDLR on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can then be measured using a chemiluminescence reader.

Lactic Acid Assay, Colorimetric/Fluorometric Assay

100 tests
EUR 320

Total Iron-Binding Capacity (TIBC) and Serum Iron Assay Kit (Colorimetric)

each
EUR 698.4

Catalase Assay

1000 tests
EUR 178

TUNEL Assay

100 tests
EUR 200

Binding Assay information

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