Competitive Atp Binding Assay

Description: The Cereblon Binding Assay Kit (FP) is designed for testing and profiling of Cereblon (CRBN) inhibitors using Fluorescence Polarization. The assay is a competitive binding assay, based on the binding of fluorescently-labeled Thalidomide, a CRBN binder, to purified recombinant CRBN._x000D_ CRBN is the substrate recognition component of a DCX (DDB1-CUL4-X-box) E3 protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins. CRBN is a direct protein target for the immunomodulatory and antiproliferative activities of thalidomide analogues such as lenalidomide and pomalidomide._x000D_ The Cereblon Binding Assay Kit (FP) comes in a convenient 96-well format, with enough purified CRBN, Cy5-labeled Thalidomide, and assay buffer for 100 enzyme reactions. In addition, the kit includes the CRBN inhibitor Pomalidomide for use as an inhibitor control._x000D_
The key to the CRBN Assay Kit is the fluorescently-labeled Thalidomide. Using this kit, only one simple step on a microtiter plate is required for CRBN reactions. The Cy5-labeled Thalidomide is incubated with a sample containing CRBN to produce a change in fluorescent polarization that can be measured using a fluorescence reader.

Description: Cell Biolabs? Aflatoxin Competitive ELISA Kit provides a convenient method for the detection of total Aflatoxin B1 and Aflatoxin B2 adducts in protein samples. First, an AFB1 conjugate is coated on an ELISA plate.  The unknown AFB1/2 samples or AFB1-BSA standards are then added to the AFB1 conjugate preabsorbed ELISA plate.  After a brief incubation, an anti-AFB1/2 monoclonal antibody is added, followed by an HRP conjugated secondary antibody.  The total content of AFB1 plus AFB2 in unknown samples is determined by comparison with a predetermined AFB1-BSA standard curve.

Description: Recombinant human Anti-OX40_x000D_competitive antibody recognizing the OX40_x000D_binding region. This antibody has not been_x000D_tested for cross-reactivity with any other_x000D_species.

Description: Recombinant human Anti-OX40 competitive antibody recognizing the OX40 binding region. This antibody has not been tested for cross-reactivity with any other species.