Pppgpp Jena Bioscience

Description: The Fluorogenic HDAC Assay Kit is a complete assay system_x000D_designed to measure histone deacetylase (HDAC) class 1 activity for screening and_x000D_profiling applications. It comes in a convenient 96-well format, with all the reagents_x000D_necessary for 100 fluorescent HDAC activity measurements. In addition, the kit_x000D_includes purified HDAC2 enzyme and a potent HDAC inhibitor, Trichostatin A,_x000D_for use as a positive and negative control. The Fluorogenic HDAC Assay_x000D_Kit is based on a unique fluorogenic substrate and developer combination. This assay_x000D_method eliminates dealing with the radioactivity, extraction, and chromatography aspects_x000D_of traditional assays. Using this kit, only two simple steps on a microtiter plate are needed_x000D_to analyze the HDAC activity level. First, the HDAC fluorometric substrate, containing_x000D_an acetylated lysine side chain, is incubated with purified HDAC enzyme._x000D_The deacetylation sensitizes the substrate so subsequent treatment with the Lysine_x000D_Developer produces a fluorophore that can then be measured using a fluorescence reader.

Description: The Fluorogenic HDAC Assay Kit is a complete assay system_x000D_designed to measure histone deacetylase (HDAC) class 1 activity for screening_x000D_and profiling applications. The kit comes in a convenient 96-well format, with all the_x000D_reagents necessary for 100 fluorescent HDAC activity measurements. In_x000D_addition, the kit includes purified HDAC2 enzyme and a potent HDAC inhibitor,_x000D_Trichostatin A, for use as a positive and negative control. The Fluorogenic HDAC_x000D_Assay Kit is based on a unique fluorogenic substrate and developer combination._x000D_This assay method eliminates dealing with the radioactivity, extraction, and_x000D_chromatography aspects of traditional assays. Using this kit, only two simple_x000D_steps on a microtiter plate are needed to analyze the HDAC activity level. First,_x000D_the HDAC fluorometric substrate, containing an acetylated lysine side chain, is_x000D_incubated with purified HDAC enzyme. The deacetylation sensitizes the_x000D_substrate so subsequent treatment with the Lysine Developer produces a_x000D_fluorophore that can then be measured using a fluorescence reader at 485 nm_x000D_(excitation)/528 nm (emission)._x000D__x000D_Our other HDAC kit (#50033) contains a substrate that is excited at wavelengths 350-380 nm and fluoresces at wavelengths 440-460 nm. However, the substrate in this kit fluoresces at longer (green) wavelengths: 485 nm(excitation)/528 nm (emission). It is most useful when the sample or inhibitor fluoresces at wavelengths that overlap those of our other HDAC substrate

Description: A fluorogenic, acetylated peptide substrate for class I HDACs (HDACs 1, 2, and 3) and class 2b HDACs (HDACs 6 and 10). This substrate fluoresces at excitation 350-380 nm, emission at 440-460 nm. If autofluorescence of the inhibitor near this wavelength is an issue, we recommend using Fluorogenic HDAC substrate 2 (green), BPS catalog #50038. For class 2a HDACs (HDACs 4, 5, 7, and 9) or HDACs 8 and 11, we recommend #50040.

Description: A fluorogenic, acetylated peptide substrate for HDACs. It is a suitable substrate for class I HDACs (HDACs 1, 2, and 3) and class 2b HDACs (HDACs 6 and 10). This substrate exhibits longer excitation and emission wavelengths (excitation 485 nm, emission 528 nm) than our regular class 1/2b HDAC substrate, #50037, which fluoresces at excitation 350-380 nm, emission at 440-460 nm. This substrate should be used when the inhibitor fluoresces near 450 nm. If autofluorescence is not an issue, we recommend HDAC substrate 3, #50037. For autofluorescent samples using HDACs 4, 5, 7, 8, 9, and 11, we recommend substrate #50042.

Description: A fluorogenic, acetylated peptide substrate for class2a HDACs (HDACs 4, 5, 7, and 9). It also works well for HDACs 8 and 11. This substrate fluoresces at excitation 350-380 nm, emission at 440-460 nm. If autofluorescence of the inhibitor near this wavelength is an issue, we recommend using Fluorogenic HDAC class 2a substrate 2 (green), BPS catalog #50042. For HDACs 1-3 ,6, and 10, we recommend substrate #50037.

Description: A fluorogenic, acetylated peptide substrate for class2a HDACs (HDACs 4, 5, 7 and 9). It also works well for HDACs 8 and 11. This substrate exhibits longer excitation and emission wavelengths (excitation 485 nm, emission 528 nm) than our regular class 2a HDAC substrate, #50040, which fluoresces at excitation 350-380 nm, emission at 440-460 nm. This substrate should be used when the inhibitor fluoresces near 450 nm. If autofluorescence is not an issue, we recommend product #50040. For autofluorescent samples using HDACs 1-3 ,6, and 10, we recommend substrate #50038.

Description: Human HDAC5 , GenBank Accession No. NM_005474, full length with N-terminal GST-tag, MW= 150 kDa, expressed in baculovirus expression system.

Description: Human HDAC6 with H216A mutation (GenBank Accession No. NM_006044), full length with N-terminal GST-tag, MW= 161 kDa, expressed in a baculovirus expression system.

Description: Human HDAC1, GenBank Accession No. NM_004964, full length with C-terminal FLAG and C-terminal His-tag, MW= 56 kDa, expressed in baculovirus expression system.

Description: Human HDAC2 , GenBank Accession No. Q92769, full length with C-terminal FLAG-tag, MW= 60 kDa, expressed in baculovirus expression system.

Description: Human HDAC6 (GenBank Accession No. NM_006044), full length with C-terminal FLAG-tag, MW= 132 kDa, expressed in baculovirus expression system.

Description: Mouse HDAC6, also known as histone deacetylase 6, GenBank Accession No. NM_010413, a.a. 2-1149 (end) with N-terminal GST-Tag, expressed in baculovirus expression system. MW = 152 kDa.

Description: Mouse HDAC1, GenBank Accession No. NM_008228, amino acids 1-482 (full length), with C-terminal His-tag and C-terminal FLAG-tag, MW = 57 kDa, expressed in an Sf9 baculovirus expression system. Recombinant HDAC1 forms a complex with endogenous Hsp70. The identity of Hsp70 was confirmed by MALDI-TOF mass spectrometry.

Description: Human HDAC10 (a.a. 2-631) (GenBank Accession No. NM_032019) with N-terminal FLAG-tag, MW= 67 kDa, expressed in Sf9 cells via a baculovirus expression system.

Description: The Fluorogenic HDAC1 Assay Kit is a complete assay system_x000D_designed to measure histone deacetylase 1 (HDAC1) activity for screening and profiling_x000D_applications. It comes in a convenient 96-well format, with all the reagents necessary for_x000D_100 fluorescent HDAC1 activity measurements. In addition, the kit includes purified_x000D_HDAC1 enzyme and a potent HDAC inhibitor, Trichostatin A, for use as a positive and_x000D_negative control. The Fluorogenic HDAC1 Assay Kit is based on a unique fluorogenic_x000D_substrate and developer combination. This assay method eliminates dealing with the_x000D_radioactivity, extraction, and chromatography aspects of traditional assays. Using this kit,_x000D_only two simple steps on a microtiter plate are needed to analyze the HDAC activity_x000D_level. First, the HDAC fluorometric substrate, containing an acetylated lysine side chain,_x000D_is incubated with purified HDAC1. The deacetylation sensitizes the substrate so_x000D_subsequent treatment with the Lysine Developer produces a fluorophore that can then_x000D_be measured using a fluorescence reader._x000D_

Description: The Fluorogenic HDAC2 Assay Kit is a complete assay system_x000D_designed to measure histone deacetylase 2 (HDAC2) activity for screening and profiling_x000D_applications. It comes in a convenient 96-well format, with all the reagents necessary for_x000D_100 fluorescent HDAC2 activity measurements. In addition, the kit includes purified_x000D_HDAC2 enzyme and a potent HDAC inhibitor, Trichostatin A, for use as a positive and_x000D_negative control. The Fluorogenic HDAC2 Assay Kit is based on a unique fluorogenic_x000D_substrate and developer combination. This assay method eliminates dealing with the_x000D_radioactivity, extraction, and chromatography aspects of traditional assays. Using this kit,_x000D_only two simple steps on a microtiter plate are needed to analyze the HDAC activity_x000D_level. First, the HDAC fluorometric substrate, containing an acetylated lysine side chain,_x000D_is incubated with purified HDAC2. The deacetylation sensitizes the substrate so_x000D_subsequent treatment with the Lysine Developer produces a fluorophore that can then_x000D_be measured using a fluorescence reader.

Description: Malarial (Plasmodium falciparum) HDAC, Genbank Accession No. AAD22407.1, full length (1-450) with C-terminal His-tag and FLAG-tag, MW=53.4 kDa, expressed in a Baculovirus infected Sf9 cell expression system.