Quantitative metabolic profiling by 1-dimensional 1 H-NMR analyses: application to plant genetics and functional genomics

Quantitative metabolic profiling by 1-dimensional 1 H-NMR analyses: application to plant genetics and functional genomics

 

Quantitative metabolic profiling by 1-dimensional

Metabolic profiling by one-dimensional (1-D) 1H-magnetic resonance (NMR) was examined for absolute quantification of soluble sugars, natural acids, amino acids and a few secondary metabolites in fruits, roots and leaves. metabolite accountable for any peak 1H-NMR spectra of pure compounds recognized from the spectra. Peak id is confirmed by the addition of small quantities of commercially obtainable pure substances. 1H-NMR computerized acquisition. 1H-NMR absolute quantification is completed with an digital reference sign synthesized and validated as in comparison with enzymatic or HPLC evaluation; the correlation coefficient between the info 1 H-NMR knowledge and HPLC enzymatic or very vital.

Relying on the species and tissues, metabolites might be measured with a 14-17 15-25 minute acquisition time. The restrict of detection is roughly 1-9 mg within the NMR tube, relying on the compound. Quantitative knowledge is used to (1) research the genetic high quality of strawberries, (2) useful research of tomato transformants expressing hexokinase and (3) a research of Arabidopsis transformants phosphoenolpyruvate carboxylase with just a few traces that confirmed a lower in enzyme exercise. biochemical phenotype of offspring strawberry fruit permits the detection of quantitative trait loci (QTL) controlling fruit high quality.

Comparability of wild-type and hexokinase roots of tomato transformants utilizing principal element evaluation of the metabolic profile revealed that environmental components, specifically tradition situations, can considerably alter the metabolic standing of the plant and thus disguise or emphasize the expression of sure genetic background. Decreased exercise of phosphoenolpyruvate carboxylase (up 75%) in Arabidopsis transformants affect on the metabolic profile with out compromising the expansion of vegetation, thus supporting the concept that the enzyme has a low affect on the carbon flux by way of the anaplerotic.

 Quantitative metabolic profiling by 1-dimensional 1 H-NMR analyses: application to plant genetics and functional genomics
Quantitative metabolic profiling by 1-dimensional 1 H-NMR analyses: software to
plant genetics and useful genomics

useful genomics within the nodules of legume plant transporter

Over the previous few many years, a mix of physiology, biochemistry, molecular and cell biology, and genetics has given us a primary understanding of among the main transportation within the office in nodules legume nitrogen, particularly these concerned within the alternate of vitamins between the contaminated cells of vegetation and rhizobia endosymbiotic them. Nevertheless, our data on this space stays uneven and scattered in lots of species of legume.

Current progress within the subject of genomics and useful genomics of two nuts Mannequin, Medicago truncatula and Lotus japonicus rapidly fill the gaps in data concerning the plant transporter gene is expressed constitutively within the nodules and different organs, and induced or in any other case specified within the nodule. The final class specifically is the main target of present efforts to grasp the particular position of the nodule-specific transporter. This quick article evaluations previous work on the biochemistry and molecular biology of plant transporter in nodules, earlier than describing current work within the subject of transcriptomics and bioinformatics.

Lastly, we take into account the useful genomics the place along with a extra classical method tends to lead us on this space of ​​analysis sooner or later. The in-silico evaluation will probably be essential when designing new experiments and interpret the brand new outcomes. Consequently microarray database and sustainable improvement they’re now simply as essential because the preliminary plant useful genomics microarray seize knowledge and analytical instruments.

Plant biologists in an effort to seize new alternatives, appreciation microarray database know-how and future developments within the integration of organic knowledge is required. The problem for plant useful genomics is to embrace new know-how in order that alternatives for vital discoveries will probably be misplaced.

Recombinant Complement Factor B (CFB)

4-RPC011Ra03
  • EUR 588.79
  • EUR 280.80
  • EUR 1877.95
  • EUR 705.98
  • EUR 1291.97
  • EUR 469.20
  • EUR 4514.88
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Rat Complement Factor B expressed in: E.coli

Recombinant Complement Factor B (CFB)

4-RPC011Ra04
  • EUR 588.79
  • EUR 280.80
  • EUR 1877.95
  • EUR 705.98
  • EUR 1291.97
  • EUR 469.20
  • EUR 4514.88
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Rat Complement Factor B expressed in: E.coli

CFB Complement Factor B Human Recombinant Protein

PROTP00751 Regular: 20ug
EUR 380.4
Description: CFB Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 528 amino acids (260-764) and having a molecular mass of 59.4 kDa.;CFB is fused to a 23 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.

Recombinant Human Complement Factor B/CFB (C-6His)

C329-10ug 10ug
EUR 187.2
Description: Supplied as a 0.2 μm filtered solution of 20mM TrisHCl, 150mM NaCl, pH 8.0.

Recombinant Human Complement Factor B/CFB (C-6His)

C329-1mg 1mg
EUR 2739.6
Description: Supplied as a 0.2 μm filtered solution of 20mM TrisHCl, 150mM NaCl, pH 8.0.

Recombinant Human Complement Factor B/CFB (C-6His)

C329-500ug 500ug
EUR 1935.6
Description: Supplied as a 0.2 μm filtered solution of 20mM TrisHCl, 150mM NaCl, pH 8.0.

Recombinant Human Complement Factor B/CFB (C-6His)

C329-50ug 50ug
EUR 442.8
Description: Supplied as a 0.2 μm filtered solution of 20mM TrisHCl, 150mM NaCl, pH 8.0.

CFB (26-259) Complement Factor B (26-259 a.a.) Human Recombinant Protein

PROTP00751-1 Regular: 20ug
EUR 380.4
Description: CFB (26-259) Human Recombinant produced in E. Coli is. a single polypeptide chain containing 257 amino acids and having a molecular mass of 28.4kDa. CFB is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

CFB Human, Complement Factor B Human Recombinant Protein, Sf9

PROTP00751-2 Regular: 10ug
EUR 380.4
Description: CFB Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 245 amino acids (26-259a.a.) and having a molecular mass of 27.3kDa (Molecular size on SDS-PAGE will appear at approximately 28-40 kDa). CFB is expressed with a 11 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.

Mouse Complement factor B (CFB)

1-CSB-EP005268MO
  • EUR 606.00
  • EUR 318.00
  • EUR 2192.40
  • EUR 919.20
  • EUR 1461.60
  • EUR 402.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Mouse Complement factor B(CFB) expressed in E.coli

Mouse Complement Factor B (CFB) Protein

20-abx066067
  • EUR 777.60
  • EUR 326.40
  • EUR 2397.60
  • EUR 927.60
  • EUR 560.40
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Mouse Complement Factor B (CFB) Protein

20-abx066070
  • EUR 777.60
  • EUR 326.40
  • EUR 2397.60
  • EUR 927.60
  • EUR 560.40
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Mouse Complement Factor B (CFB) CLIA Kit

20-abx493368
  • EUR 9567.60
  • EUR 5095.20
  • EUR 1177.20
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Mouse Complement Factor B (CFB) CLIA Kit

20-abx496333
  • EUR 10282.80
  • EUR 5472.00
  • EUR 1262.40
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Mouse Complement Factor B (CFB) ELISA Kit

20-abx258742
  • EUR 8684.40
  • EUR 4626.00
  • EUR 1074.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Mouse Complement Factor B (CFB) ELISA Kit

abx255258-96tests 96 tests
EUR 848.4

Mouse Complement Factor B (CFB) ELISA Kit

abx573207-96tests 96 tests
EUR 848.4

Mouse Complement Factor B (CFB) ELISA Kit

abx352700-96tests 96 tests
EUR 943.2

Mouse Complement Factor B (CFB) ELISA Kit

DLR-CFB-Mu-48T 48T
EUR 632.4
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Complement Factor B (CFB) in samples from serum, plasma or other biological fluids.

Mouse Complement Factor B (CFB) ELISA Kit

DLR-CFB-Mu-96T 96T
EUR 825.6
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Complement Factor B (CFB) in samples from serum, plasma or other biological fluids.

Mouse Complement Factor B (CFB) ELISA Kit

20-abx153859
  • EUR 8853.60
  • EUR 4719.60
  • EUR 1093.20
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Mouse Complement factor B, Cfb ELISA KIT

ELI-06462m 96 Tests
EUR 1038

Mouse Complement Factor B (CFB) ELISA Kit

SEC011Mu-10x96wellstestplate 10x96-wells test plate
EUR 5834.88
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Complement Factor B (CFB) in serum, plasma and other biological fluids.

Mouse Complement Factor B (CFB) ELISA Kit

SEC011Mu-1x48wellstestplate 1x48-wells test plate
EUR 585.7
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Complement Factor B (CFB) in serum, plasma and other biological fluids.

Mouse Complement Factor B (CFB) ELISA Kit

SEC011Mu-1x96wellstestplate 1x96-wells test plate
EUR 785.28
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Complement Factor B (CFB) in serum, plasma and other biological fluids.

Mouse Complement Factor B (CFB) ELISA Kit

SEC011Mu-5x96wellstestplate 5x96-wells test plate
EUR 3173.76
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Complement Factor B (CFB) in serum, plasma and other biological fluids.

Mouse Complement Factor B (CFB) ELISA Kit

4-SEC011Mu
  • EUR 5895.60
  • EUR 3114.00
  • EUR 786.00
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Mouse Complement Factor B (CFB) in samples from serum, plasma and other biological fluids with no significant corss-reactivity with analogues from other species.

Mouse Complement Factor B (CFB) ELISA Kit

RDR-CFB-Mu-48Tests 48 Tests
EUR 668.4

Mouse Complement Factor B (CFB) ELISA Kit

RDR-CFB-Mu-96Tests 96 Tests
EUR 928.8

Mouse Complement Factor B(CFB)ELISA Kit

QY-E20368 96T
EUR 433.2

Mouse Complement Factor B (CFB) ELISA Kit

RD-CFB-Mu-48Tests 48 Tests
EUR 639.6

Mouse Complement Factor B (CFB) ELISA Kit

RD-CFB-Mu-96Tests 96 Tests
EUR 888

Mouse Complement Factor B (CFB) ELISA Kit

RD-CFB-Mu-48T 48T
EUR 464.3
Description: serum, plasma and other biological fluids.

Mouse Complement Factor B (CFB) ELISA Kit

RD-CFB-Mu-96T 96T
EUR 663.3
Description: serum, plasma and other biological fluids.

Mouse Complement Factor B (CFB) ELISA Kit

RDR-CFB-Mu-48T 48T
EUR 487.52
Description: serum, plasma and other biological fluids.

Mouse Complement Factor B (CFB) ELISA Kit

RDR-CFB-Mu-96T 96T
EUR 696.47
Description: serum, plasma and other biological fluids.

Mouse Complement Factor B ELISA Kit (CFB)

RK02680 96 Tests
EUR 625.2

Mouse Cfb/ Complement factor B ELISA Kit

E0276Mo 1 Kit
EUR 685.2

Mouse CFB(Complement Factor B) ELISA Kit

EM0918 96T
EUR 628.92
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Mus ;Sensitivity: 0.938 ng/ml

Complement Factor B Recombinant Protein

91-305 0.05 mg
EUR 651.3
Description: Complement Factor B (CFB) belongs to the peptidase S1 family of enzymes. It is expressed by hepatocytes and macrophages and localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. CFB which is a component of the alternate pathway of the complement system is cleaved by factor D into 2 fragments: Ba and Bb. Bb. The active subunit Bb is a serine protease which associates with C3b to form the alternative pathway C3 convertase. Bb is involved in the proliferation of preactivated B lymphocytes, while Ba inhibits their proliferation.

Recombinant Mouse complement factor B Protein, His, E.coli-1mg

QP5822-ec-1mg 1mg
EUR 2763.6

Recombinant Mouse complement factor B Protein, His, E.coli-10ug

QP5822-ec-10ug 10ug
EUR 326.4

Recombinant Mouse complement factor B Protein, His, E.coli-50ug

QP5822-ec-50ug 50ug
EUR 434.4

Recombinant Mouse complement factor B Protein, His, E.coli-100ug

QP5822-ec-100ug 100ug
EUR 685.2

Recombinant Mouse complement factor B Protein, His, E.coli-200ug

QP5822-ec-200ug 200ug
EUR 1077.6

Recombinant Mouse complement factor B Protein, His, E.coli-500ug

QP5822-ec-500ug 500ug
EUR 1816.8

Complement Factor B (CFB) Antibody

abx231853-100ug 100 ug
EUR 577.2

Complement Factor B (CFB) Antibody

abx231854-100ug 100 ug
EUR 577.2

Complement Factor B (CFB) Antibody

20-abx109669
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Complement Factor B (CFB) Antibody

20-abx101730
  • EUR 510.00
  • EUR 159.60
  • EUR 1446.00
  • EUR 693.60
  • EUR 393.60
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Complement Factor B (CFB) Antibody

20-abx101731
  • EUR 526.80
  • EUR 159.60
  • EUR 1479.60
  • EUR 710.40
  • EUR 393.60
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Complement Factor B (CFB) Antibody

20-abx101732
  • EUR 526.80
  • EUR 159.60
  • EUR 1479.60
  • EUR 710.40
  • EUR 393.60
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Complement Factor B (CFB) Antibody

20-abx101733
  • EUR 543.60
  • EUR 159.60
  • EUR 1562.40
  • EUR 744.00
  • EUR 410.40
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Complement Factor B (CFB) Antibody

20-abx101734
  • EUR 543.60
  • EUR 159.60
  • EUR 1562.40
  • EUR 744.00
  • EUR 410.40
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Complement Factor B (CFB) Antibody

20-abx101735
  • EUR 543.60
  • EUR 159.60
  • EUR 1562.40
  • EUR 744.00
  • EUR 410.40
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Complement Factor B (CFB) Antibody

20-abx171896
  • EUR 410.40
  • EUR 1028.40
  • EUR 526.80
  • EUR 184.80
  • EUR 309.60
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Complement Factor B (CFB) Antibody

20-abx325497
  • EUR 376.80
  • EUR 292.80
  • 100 ug
  • 50 ug

Complement Factor B (CFB) Antibody

20-abx111771
  • EUR 878.40
  • EUR 477.60
  • 150 ul
  • 50 ul

Complement Factor B (CFB) Antibody

20-abx015584
  • EUR 376.80
  • EUR 117.60
  • EUR 477.60
  • EUR 594.00
  • 100 ug
  • 10 ug
  • 200 ug
  • 300 µg

Complement Factor B (CFB) Antibody

20-abx101728
  • EUR 510.00
  • EUR 159.60
  • EUR 1446.00
  • EUR 693.60
  • EUR 393.60
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Complement Factor B (CFB) Antibody

20-abx101729
  • EUR 510.00
  • EUR 159.60
  • EUR 1446.00
  • EUR 693.60
  • EUR 393.60
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Complement Factor B (CFB) Antibody

20-abx130136
  • EUR 543.60
  • EUR 159.60
  • EUR 1562.40
  • EUR 744.00
  • EUR 410.40
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Complement Factor B (CFB) Antibody

20-abx338956
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Complement Factor B (CFB) Antibody

abx340008-100ug 100 ug
EUR 469.2

Complement Factor B (CFB) Antibody

20-abx001430
  • EUR 493.20
  • EUR 710.40
  • EUR 218.40
  • EUR 376.80
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

Complement Factor B (CFB) Polyclonal Antibody (Mouse)

4-PAC011Mu01
  • EUR 301.20
  • EUR 3091.20
  • EUR 768.00
  • EUR 379.20
  • EUR 258.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Complement Factor B (CFB)

ELISA kit for Mouse CFB (Complement Factor B)

E-EL-M0334 1 plate of 96 wells
EUR 640.8
Description: A sandwich ELISA kit for quantitative measurement of Mouse CFB (Complement Factor B) in samples from Serum, Plasma, Cell supernatant

ELISA kit for Mouse CFB (Complement Factor B)

ELK5822 1 plate of 96 wells
EUR 518.4
Description: A sandwich ELISA kit for detection of Complement Factor B from Mouse in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

Complement Factor B Antibody / CFB

R32391 100 ug
EUR 356.15
Description: Complement factor B is a protein that in humans is encoded by the CFB gene. This gene encodes complement factor B, a component of the alternative pathway of complement activation. Factor B circulates in the blood as a single chain polypeptide. Upon activation of the alternative pathway, it is cleaved by complement factor D yielding the noncatalytic chain Ba and the catalytic subunit Bb. The active subunit Bb is a serine protease which associates with C3b to form the alternative pathway C3 convertase. Bb is involved in the proliferation of preactivated B lymphocytes, while Ba inhibits their proliferation. This gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. This cluster includes several genes involved in regulation of the immune reaction. Polymorphisms in this gene are associated with a reduced risk of age-related macular degeneration. The polyadenylation site of this gene is 421 bp from the 5' end of the gene for complement component 2.

Complement Factor B (CFB) ELISA Kit

QY-E120010 96T
EUR 573.6

Complement Factor B (CFB) Polyclonal Antibody (Mouse), PE

4-PAC011Mu01-PE
  • EUR 361.20
  • EUR 3254.40
  • EUR 921.60
  • EUR 454.80
  • EUR 236.40
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Complement Factor B (CFB). This antibody is labeled with PE.

Complement Factor B (CFB) Polyclonal Antibody (Mouse), APC

4-PAC011Mu01-APC
  • EUR 421.20
  • EUR 4038.00
  • EUR 1122.00
  • EUR 538.80
  • EUR 266.40
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Complement Factor B (CFB). This antibody is labeled with APC.

Complement Factor B (CFB) Polyclonal Antibody (Mouse), Cy3

4-PAC011Mu01-Cy3
  • EUR 512.40
  • EUR 5334.00
  • EUR 1446.00
  • EUR 668.40
  • EUR 304.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Complement Factor B (CFB). This antibody is labeled with Cy3.

Complement Factor B (CFB) Polyclonal Antibody (Mouse), HRP

4-PAC011Mu01-HRP
  • EUR 385.20
  • EUR 3519.60
  • EUR 992.40
  • EUR 486.00
  • EUR 250.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Complement Factor B (CFB). This antibody is labeled with HRP.

Complement Factor B (CFB) Polyclonal Antibody (Mouse, Rat)

4-PAC011Mu02
  • EUR 301.20
  • EUR 3091.20
  • EUR 768.00
  • EUR 379.20
  • EUR 258.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse, Rat Complement Factor B (CFB)

Complement Factor B (CFB) Polyclonal Antibody (Mouse, Rat)

4-PAC011Ra03
  • EUR 310.80
  • EUR 3249.60
  • EUR 804.00
  • EUR 393.60
  • EUR 262.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse, Rat Complement Factor B (CFB)

Rat Complement Factor B (CFB) Protein

20-abx168489
  • EUR 828.00
  • EUR 343.20
  • EUR 2532.00
  • EUR 978.00
  • EUR 594.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Rat Complement Factor B (CFB) Protein

20-abx066068
  • EUR 828.00
  • EUR 343.20
  • EUR 2532.00
  • EUR 978.00
  • EUR 594.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Rat Complement Factor B (CFB) Protein

20-abx066071
  • EUR 828.00
  • EUR 343.20
  • EUR 2532.00
  • EUR 978.00
  • EUR 594.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Rat Complement Factor B (CFB) Protein

20-abx066072
  • EUR 828.00
  • EUR 343.20
  • EUR 2532.00
  • EUR 978.00
  • EUR 594.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

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