Genomic screen identifies a role for the plasmid-borne type II secretion system

 

Genomic screen identifies a role for the plasmid-borne type II

Shiga-toxigenic Escherichia coli (STEC) is often transmitted to the food through fresh products plant, where it can cause disease. To identify factors for STEC early interaction in spinach, positive system used high-throughput selection. An artificial chromosome (BAC) clone library to isolate bacteria Sakai played in four successive rounds of short-term (2 h) interaction with the roots of spinach, and enriched loci identified by microarray.

A Bayesian hierarchical model produced 115 CDS credible candidates, which consists of seven contiguous genomic region. Of the two regions selected candidates for functional assessment, pO157 two plasmid-encoded type secretion system (T2SS) interaction promoted, while fimbrial chaperone-usher gene cluster (loc6) does. The T2SS promoted spinach binds bacteria and appears to involve a protein EtpD secretin. Furthermore, the gene T2SS, etpD and etpC disclosed in the relevant crop temperature of 18 ° C, and etpD expressed in planta by E. coli in spinach plants Sakai.

During the early embryogenesis of plants, some of the most fundamental decision on the fate and identity are taken so that the process is interesting to study. It is not surprising that higher plant embryogenesis intensively analyzed during the last century, while the somatic embryogenesis probably the most studied model of regeneration. Encoded by the miRNA, short, single-stranded, non-coding miRNAs, are generally present in all eukaryotic genomes and are involved in the regulation of gene expression during critical developmental processes such as morphogenesis of plants, hormone signaling, and the transition phase of development.

Over the last few years dedicated to miRNAs, analysis methods and tools have been developed, which has been given new opportunities in functional analysis of plant miRNAs, including (i) database for in silico analysis; (Ii) detection of the expression of miRNAs and approaches; (Iii) the reporter and the line sensor for spatio-temporal analysis of the interaction of miRNA targets; (Iv) in the in situ hybridization protocol; (V) artificial miRNAs; (Vi) MIM and STTM line to inhibit miRNA activity, and (vii) of target genes that are resistant to miRNA. Here, we attempt to summarize the toolbox for functional analysis of miRNAs during plant embryogenesis. In addition to the characteristics described tools / methods, application examples have been presented.

functional genomics in the nodules of legume plant transporter

Over the last few decades, a combination of physiology, biochemistry, molecular and cell biology, and genetics has given us a basic understanding of some of the major transportation in the workplace in nodules legume nitrogen, especially those involved in the exchange of nutrients between the infected cells of plants and rhizobia endosymbiotic them.

However, our knowledge in this area remains uneven and scattered in many species of legume. Recent progress in the field of genomics and functional genomics of two nuts Model, Medicago truncatula and Lotus japonicus quickly fill the gaps in knowledge about the plant transporter gene is expressed constitutively in the nodules and other organs, and induced or otherwise specified in the nodule.

The last class in particular is the focus of current efforts to understand the special role of the nodule-specific transporter. This short article reviews past work on the biochemistry and molecular biology of plant transporter in nodules, before describing recent work in the field of transcriptomics and bioinformatics. Finally, we consider the functional genomics where together with a more classical approach tends to lead us in this area of ​​research in the future.

Rat Bladder PrimaCell2: Normal Bladder Fibroblasts
2-82507	CHI Scientific	1 Kit	Ask for price

Human Bladder PrimaCell2: Normal Bladder Fibroblasts
2-96008	CHI Scientific	1 Kit	Ask for price

Bladder cancer with bladder tissue array
BL2081C	TissueArray	each	EUR 545
Description: Bladder cancer with bladder tissue array, including pathology grade, TNM and clinical stage, 191 cases/192 cores, replacing BL2081b

Bladder cancer with bladder tissue array
BL243a	TissueArray	each	EUR 78
Description: Bladder cancer with bladder tissue array, including urothelial carcinoma,squamous cell carcinoma, adenocarcinoma and normal tissue, pathololy grade, TNM and clinical stage (AJCC 8.0), 12 case/24 cores (1.5mm), replacing BL243

Bladder cancer with bladder tissue array
BL481d	TissueArray	each	EUR 198
Description: Bladder cancer with bladder tissue array, including urothelial carcinoma, squamous cell carcinoma, adenocarcinoma and normal tissue, TNM/Stage, 48 cases/48 cores (core size 2.0 mm), replacing BL481d

Bladder cancer and bladder tissue array
BL806b	TissueArray	each	EUR 306
Description: Bladder cancer and bladder tissue array, including pathology grade, TNM and clinical stage, 60 cases/80 cores, replacing BL806a

Bladder cancer with bladder tissue array
BL807	TissueArray	each	EUR 306
Description: Bladder cancer with bladder tissue array, including TNM and clinical stage, 80 cases/80 cores

Bladder cancer with bladder tissue array
T124c	TissueArray	each	EUR 78
Description: Bladder cancer with bladder tissue array, including pathology grade, TNM and clinical stage, 12 cases/24 cores, replacing T124b

Bladder Lysate
1410	ProSci	0.1 mg	EUR 229.2
Description: Bladder tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Bladder Lysate
1478	ProSci	0.1 mg	EUR 229.2
Description: Bladder tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Mouse Bladder PrimaCell1: Normal Bladder Epithelial Cells
2-82006	CHI Scientific	1 Kit	Ask for price

Rat Bladder PrimaCell1: Normal Bladder Epithelial Cells
2-82506	CHI Scientific	1 Kit	Ask for price

Human Bladder PrimaCell1: Normal Bladder Epithelial Cells
2-96007	CHI Scientific	1 Kit	Ask for price

Multiple bladder carcinoma with bladder tissue array
BL1501a	TissueArray	each	EUR 258
Description: Multiple bladder carcinoma with bladder tissue array, including pathology grade, TNM and clinical stage, 75 cases/150 cores, replacing BL1501

Bladder cancer with bladder tissue test array
T121b	TissueArray	each	EUR 66
Description: Bladder cancer with bladder tissue test array, including pathology grade, TNM and clinical stage, 6 cases/24 cores, replacing T121a

Human Bladder PrimaCell3: Normal Bladder Smooth Muscle Cells
2-96009	CHI Scientific	1 Kit	Ask for price

Rat Bladder PrimaCell2: Normal Bladder Fibroblasts Growth Medium
9-25007	CHI Scientific	5 x 100 ml	Ask for price

Mouse Bladder PrimaCell2: Normal Bladder Fibroblasts Growth Medium
9-32007	CHI Scientific	5 x 100 ml	Ask for price

Human Bladder PrimaCell2: Normal Bladder Fibroblasts Growth Medium
9-46008	CHI Scientific	5 x 100 ml	Ask for price

Bladder cancer tissue array with normal bladder tissue
BL481b	TissueArray	each	EUR 198
Description: Bladder cancer tissue array with normal bladder tissue, including pathology grade, TNM and clinical stage, 48 cases/48 cores, replaced by BL481c

Bladder Membrane Lysate
XBL-10475	ProSci	0.1 mg	EUR 619.8
Description: Human bladder tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human bladder tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated bladder tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated bladder tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.

Rat Bladder PrimaCell1: Normal Bladder Epithelial Cells Growth Medium
9-25006	CHI Scientific	5 x 100 ml	Ask for price

Mouse Bladder PrimaCell1: Normal Bladder Epithelial Cells Growth Medium
9-32006	CHI Scientific	5 x 100 ml	Ask for price

Human Bladder PrimaCell1: Normal Bladder Epithelial Cells Growth Medium
9-46007	CHI Scientific	5 x 100 ml	Ask for price

Human Bladder Tissue Preparation Buffer 2: Normal Bladder Fibroblasts
9-80008	CHI Scientific	1 x 100 ml	Ask for price

Mouse Bladder Tissue Preparation Buffer 2: Normal Bladder Fibroblasts
9-80121	CHI Scientific	1 x 100 ml	Ask for price

Rat Bladder Tissue Preparation Buffer 2: Normal Bladder Fibroblasts
9-80214	CHI Scientific	1 x 100 ml	Ask for price

Bladder cancer with bladder tissue array (2016 WHO classification)
BL208a	TissueArray	each	EUR 546
Description: Bladder cancer with bladder tissue array (2016 WHO classification), including pathology grade, TNM and clinical stage (AJCC 7th edition), 64 cases/192 cores, replacing BL208

Bladder invasive urothelial carcinoma with matched bladder tissue array
BL244b	TissueArray	each	EUR 66
Description: Bladder invasive urothelial carcinoma with matched bladder tissue array, including pathology grade, TNM and clinical stage, 12 cases/24 cores (1.5mm), replacing BL244a

Hamster IgM
31C-CH0707	Fitzgerald	1 mg	EUR 492
Description: Purified Hamster IgM

Human Bladder PrimaCell3: Normal Bladder Smooth Muscle Cells Growth Medium
9-46009	CHI Scientific	5 x 100 ml	Ask for price

Human Bladder Tissue Preparation Buffer 1: Normal Bladder Epithelial Cells
9-80007	CHI Scientific	1 x 100 ml	Ask for price

Mouse Bladder Tissue Preparation Buffer 1: Normal Bladder Epithelial Cells
9-80120	CHI Scientific	1 x 100 ml	Ask for price

Rat Bladder Tissue Preparation Buffer 1: Normal Bladder Epithelial Cells
9-80213	CHI Scientific	1 x 100 ml	Ask for price

Bladder urothelial carcinoma with matched cancer adjacent bladder tissue array
BL241b	TissueArray	each	EUR 66
Description: Bladder urothelial carcinoma with matched cancer adjacent bladder tissue array, including TNM and clinical stage, 10 cases/24 cores, replacing BL241a

Bladder cancer tissue array with matched adjacent normal bladder tissue
BL631	TissueArray	each	EUR 306
Description: Bladder cancer tissue array with matched adjacent normal bladder tissue, including TNM and pathology grade, 24 cases/63 cores

Bladder disease spectrum (urocystic cancer progression) with bladder tissue array
BL804	TissueArray	each	EUR 306
Description: Bladder disease spectrum (urocystic cancer progression) with bladder tissue array, including pathology grade, TNM and clinical stage, 80 cases/80 cores

Bladder Tissue Slide (Normal)
12-601-10um	ProSci	10 um	EUR 241.8

Bladder Tissue Slide (Normal)
12-601-4um	ProSci	4 um	EUR 216.6

Bladder Tissue Lysate (Normal)
1721-01	ProSci	0.1 mg	EUR 260.7
Description: Bladder tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Bladder Tissue Lysate (Tumor)
1722-01	ProSci	0.1 mg	EUR 336.3
Description: Bladder tumor tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Bladder Tissue Lysate (Tumor)
1722-02	ProSci	0.1 mg	EUR 336.3
Description: Bladder tumor tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Human Bladder Tumor lysate
HTL-1325	Alpha Diagnostics	1 mg	EUR 927.6

Bladder cancer tissue array
BC12011c	TissueArray	each	EUR 186
Description: Bladder cancer tissue array, including TNM, 63 cases/63 cores (core size 1.5mm)

Bladder cancer tissue array
BL2082a	TissueArray	each	EUR 546
Description: Bladder cancer tissue array, including TNM, clinical stage and pathology grade, 208 cases/ 208 cores, replacing BL2082

Bladder cancer tissue array
BL2082B	TissueArray	each	EUR 546
Description: Bladder cancer tissue array, including TNM and clinical stage, 208 cases/208 cores, replacing BL2082a

Bladder cancer tissue array
BL242	TissueArray	each	EUR 270
Description: Bladder cancer tissue array, with normal tissue, including TNM and pathology grade, 24 cases/48 cores

Normal bladder tissue array
BNC12011	TissueArray	each	EUR 222
Description: Normal bladder tissue array, including TNM, clinical stage and pathology grade, 40 cases/ 80 cores

cDNA Synthesis SuperMix
20-abx09801420ulSystems	Abbexa	EUR 678.00 EUR 577.20	100 rxns × 20 ul Systems 50 rxns × 20 ul Systems

Novo? cDNA Kit
M1165-100	Biovision	each	EUR 424.8

Novo? cDNA Kit
M1165-25	Biovision	each	EUR 320.4

Evo? cDNA Supermix
M1168-100	Biovision	each	EUR 457.2

Evo? cDNA Supermix
M1168-25	Biovision	each	EUR 320.4

Novo? cDNA Supermix
M1169-100	Biovision	each	EUR 529.2

Novo? cDNA Supermix
M1169-25	Biovision	each	EUR 346.8

Human Bladder Tissue Preparation Buffer 3: Normal Bladder Smooth Muscle Cells
9-80009	CHI Scientific	1 x 100 ml	Ask for price

cDNA from Plant Normal Tissue: cDNA from Plant: Arabidopsis
C1634310	Biochain	40 reactions	EUR 745.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Corn
C1634330	Biochain	40 reactions	EUR 745.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Orange
C1634340	Biochain	40 reactions	EUR 745.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Potato
C1634350	Biochain	40 reactions	EUR 745.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Rice
C1634360	Biochain	40 reactions	EUR 745.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Wheat
C1634390	Biochain	40 reactions	EUR 745.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Hamster Mono Anti-Mouse CD3e IgG (Hamster IgG)
MCD003E-M	Alpha Diagnostics	100 Tests	EUR 578.4

Hamster Mono Anti-Mouse CD3z IgG (Hamster IgG)
MCD003Z-M	Alpha Diagnostics	100 ug	EUR 578.4

Bladder Tumor Tissue Array - 12 cases of bladder tumor/adjacent normal pairs
Z7020105	Biochain	5 slides	EUR 1657.2
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Hamster IgG (FITC)
65C-CH0702	Fitzgerald	1 mg	EUR 240
Description: Purified Hamster IgG FITC conjugate

Hamster IgG (HRP)
65C-CH0703	Fitzgerald	1 mg	EUR 296.4
Description: Purified Hamster IgG HRP conjugate

Hamster Ig fraction
31R-1009	Fitzgerald	10 mg	EUR 165.6
Description: Hamster Ig fraction control or blocking reagent

Hamster Lymphocyte antibody
20R-LR011	Fitzgerald	20 mg	EUR 392.4
Description: Rabbit polyclonal Hamster Lymphocyte antibody

Hamster Lymphocyte antibody
20R-LR012	Fitzgerald	2 ml	EUR 289.2
Description: Rabbit polyclonal Hamster Lymphocyte antibody

Hamster RBC antibody
20R-RR021	Fitzgerald	20 mg	EUR 392.4
Description: Rabbit polyclonal Hamster RBC antibody

Hamster RBC antibody
20R-RR022	Fitzgerald	5 mg	EUR 664.8
Description: Rabbit polyclonal Hamster RBC antibody

Hamster Complement Serum
32R-CH001	Fitzgerald	5 ml	EUR 508.8
Description: Purified normal Hamster Complement isolated from blood

Hamster CD28 Antibody
abx139999-01mg	Abbexa	0.1 mg	EUR 343.2

Hamster CD3 Antibody
abx140000-01mg	Abbexa	0.1 mg	EUR 343.2

Hamster CD3 Antibody
abx140001-01mg	Abbexa	0.1 mg	EUR 343.2

Hamster CD80 Antibody
abx140014-01mg	Abbexa	0.1 mg	EUR 343.2

Hamster CD154 Antibody
abx140091-01mg	Abbexa	0.1 mg	EUR 410.4

Hamster CD154 Antibody
abx140092-01mg	Abbexa	0.1 mg	EUR 343.2

Hamster Helios Antibody
abx140094-01mg	Abbexa	0.1 mg	EUR 493.2

Recombinant Hamster Uter
P0138	FN Test	100ug	EUR 626.83
Description: Recombinant Hamster protein for Uter

Hamster Transthyretin (TTR)
QY-E90097	Qayee Biotechnology	96T	EUR 573.6

Bladder Tissue Slide (papillary Carcinoma)
12-612-10um	ProSci	10 um	EUR 241.8

Bladder Tissue Slide (papillary Carcinoma)
12-612-4um	ProSci	4 um	EUR 216.6

Mouse Bladder Whole tissue lysate
MAL-1410	Alpha Diagnostics	1 mg	EUR 927.6

Human Bladder Cancer Primer Library
HBLCP-I	Real Time Primers	1 set	EUR 657.6

Human Bladder Microvascular Endothelial Cells
HEC21	Neuromics	500,000 + Cells Cryoperserved	EUR 1296

Rat Bladder Whole tissue lysate
RAL-1478	Alpha Diagnostics	1 mg	EUR 628.8

First-Strand cDNA Synthesis SuperMix (cDNA up to 12 kb)
20-abx09801620ulSystems	Abbexa	EUR 744.00 EUR 627.60	100 rxns × 20 ul Systems 50 rxns × 20 ul Systems

First-Strand cDNA Synthesis SuperMix (cDNA up to 15 kb)
20-abx09802120ulSystems	Abbexa	EUR 1045.20 EUR 794.40	100 rxns × 20 ul Systems 50 rxns × 20 ul Systems

cDNA from Plant Normal Tissue: cDNA from Plant: Soy bean
C1634370	Biochain	40 reactions	EUR 745.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Tetro cDNA Synthesis Kit
BIO-65042	Bioline	30 Reactions	Ask for price

Tetro cDNA Synthesis Kit
BIO-65043	Bioline	100 Reactions	Ask for price

SensiFAST cDNA Synthesis Kit
BIO-65053	Bioline	50 Reactions	Ask for price

SensiFAST cDNA Synthesis Kit
BIO-65053/S	Bioline	Sample	Ask for price

SensiFAST cDNA Synthesis Kit
BIO-65054	Bioline	250 Reactions	Ask for price