Genomic screen identifies a role for the plasmid-borne type II secretion system

A high-throughput genomic screen identifies a role for the plasmid-borne type II secretion system of Escherichia coli O157:H7 (Sakai) in plant-microbe interactions

 

Genomic screen identifies a role for the plasmid-borne type II

Shiga-toxigenic Escherichia coli (STEC) is often transmitted to the food through fresh products plant, where it can cause disease. To identify factors for STEC early interaction in spinach, positive system used high-throughput selection. An artificial chromosome (BAC) clone library to isolate bacteria Sakai played in four successive rounds of short-term (2 h) interaction with the roots of spinach, and enriched loci identified by microarray.

A Bayesian hierarchical model produced 115 CDS credible candidates, which consists of seven contiguous genomic region. Of the two regions selected candidates for functional assessment, pO157 two plasmid-encoded type secretion system (T2SS) interaction promoted, while fimbrial chaperone-usher gene cluster (loc6) does. The T2SS promoted spinach binds bacteria and appears to involve a protein EtpD secretin. Furthermore, the gene T2SS, etpD and etpC disclosed in the relevant crop temperature of 18 ° C, and etpD expressed in planta by E. coli in spinach plants Sakai.


During the early embryogenesis of plants, some of the most fundamental decision on the fate and identity are taken so that the process is interesting to study. It is not surprising that higher plant embryogenesis intensively analyzed during the last century, while the somatic embryogenesis probably the most studied model of regeneration. Encoded by the miRNA, short, single-stranded, non-coding miRNAs, are generally present in all eukaryotic genomes and are involved in the regulation of gene expression during critical developmental processes such as morphogenesis of plants, hormone signaling, and the transition phase of development.

Over the last few years dedicated to miRNAs, analysis methods and tools have been developed, which has been given new opportunities in functional analysis of plant miRNAs, including (i) database for in silico analysis; (Ii) detection of the expression of miRNAs and approaches; (Iii) the reporter and the line sensor for spatio-temporal analysis of the interaction of miRNA targets; (Iv) in the in situ hybridization protocol; (V) artificial miRNAs; (Vi) MIM and STTM line to inhibit miRNA activity, and (vii) of target genes that are resistant to miRNA. Here, we attempt to summarize the toolbox for functional analysis of miRNAs during plant embryogenesis. In addition to the characteristics described tools / methods, application examples have been presented.

 A high-throughput genomic screen identifies a role for the plasmid-borne type II secretion system of Escherichia coli O157:H7 (Sakai) in plant-microbe interactions
A high-throughput genomic screen identifies a role for the plasmid-borne type II secretion system of Escherichia coli O157:H7 (Sakai) in plant-microbe interactions

cDNA - Monkey (Cynomolgus) Normal Tissue: Colon

C1534090-Cy 40 reactions
EUR 385

cDNA - Human Adult Normal Tissue: Colon Sigmoid

C1234095 40 reactions
EUR 385

cDNA - Monkey (Rhesus) Normal Tissue: Colon Sigmoid

C1534095 40 reactions
EUR 578

cDNA - Human Adult Normal Tissue: Colon Ascending

C1234091 40 reactions
EUR 385

cDNA - Human Adult Normal Tissue: Colon Descending

C1234092 40 reactions
EUR 385

cDNA - Human Adult Normal Tissue: Colon Transverse

C1234096 40 reactions
EUR 385

cDNA - Monkey (Rhesus) Normal Tissue: Colon Ascending

C1534091 40 reactions
EUR 578

cDNA - Monkey (Rhesus) Normal Tissue: Colon descending

C1534092 40 reactions
EUR 578

cDNA - Monkey (Rhesus) Normal Tissue: Colon Transverse

C1534096 40 reactions
EUR 578

Genorise® Bovine IL-1b cDNA Clone, 10 ug

GR152001 10 µg
EUR 153

Matched Pair - cDNA - Human Primary Tumor and Normal Tissue: Colon

C8235090-PP 10 reactions x2
EUR 530

Matched Pair - cDNA - Human Primary and Matched Metastatic Tumor Tissue: Colon

C8235090-PM 10 reactions x2
EUR 1102

Colon

TMA2081 each
EUR 546
Description: Colon, prostate and breast tissue array, 156 cases/208 cores

Bovine Granulocyte colony-stimulating factor(CSF3) ELISA kit

CSB-EL006049BO-24T 1 plate of 24 wells
EUR 198
Description: Quantitativesandwich ELISA kit for measuring Bovine Granulocyte colony-stimulating factor (CSF3) in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Bovine Granulocyte colony-stimulating factor(CSF3) ELISA kit

1-CSB-EL006049BO
  • EUR 843.60
  • EUR 5811.60
  • EUR 3084.00
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitativesandwich ELISA kit for measuring Bovine Granulocyte colony-stimulating factor(CSF3) in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Bovine Granulocyte Colony Stimulating Factor,G-CSF ELISA Kit

QY-E60021 96T
EUR 511.2

Bovine Granulocyte macrophage colony stimulating factor, GM- CSF

ELA-E0045b 96 Tests
EUR 1113.6

Colon cancer with colon tissue array

CO1021a each
EUR 306
Description: Colon cancer with colon tissue array, including pathology grade, with MMR (MLH1/PMS2/MSH2/MSH6) IHC results,TNM and clinical stage, containing 86 cases of adenocarcinoma, 4 papillary adenocarcinoma, 7 mucinous adenocarcinoma, 2 adenoma, 1 congenital megacolon, 2 colon tissue, 102 cases/102 cores replacing CO1021

Colon cancer with colon tissue array

CO1921 each
EUR 546
Description: Colon cancer with colon tissue array, including pathology grade, TNM and clinical stage, 192 cases/192 cores

Colon cancer with colon tissue array

CO2161b each
EUR 474
Description: Colon cancer with colon tissue array, including pathology grade, TNM and clinical stage, 216 cases/216 cores, replacing CO2161a

Colon cancer with colon tissue array

CO721 each
EUR 270
Description: Colon cancer with colon tissue array, including pathology grade, TNM and clinical stage, 24 cases/72 cores

Colon cancer with colon tissue array

CO805 each
EUR 258
Description: Colon cancer with colon tissue array, including pathology grade, TNM and clinical stage, 40 cases/80 cores

Colon adenocarcinoma with colon tissue array

CO483b each
EUR 198
Description: Colon adenocarcinoma with colon tissue array, including pathology, TNM and clinical stage, 48 cases/48 cores, replacing CO483a

Colony Stimulating Factor 3, Granulocyte (GCSF) Polyclonal Antibody (Bovine)

4-PAA042Bo01
  • EUR 310.80
  • EUR 3232.80
  • EUR 800.40
  • EUR 391.20
  • EUR 261.60
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Bovine Colony Stimulating Factor 3, Granulocyte (GCSF)

Bovine GM-CSF(Granulocyte Macrophage Colony Stimulating Factor) ELISA Kit

EB0161 96T
EUR 681.12
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Cattle;Sensitivity: 9.375pg/ml

Bovine Granulocyte-macrophage colony-stimulating factor(CSF2) ELISA kit

CSB-EL006045BO-24T 1 plate of 24 wells
EUR 198
Description: Quantitativesandwich ELISA kit for measuring Bovine Granulocyte-macrophage colony-stimulating factor (CSF2) in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Bovine Granulocyte-macrophage colony-stimulating factor(CSF2) ELISA kit

1-CSB-EL006045BO
  • EUR 1080.00
  • EUR 6571.20
  • EUR 3480.00
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitativesandwich ELISA kit for measuring Bovine Granulocyte-macrophage colony-stimulating factor(CSF2) in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Bovine CSF2/ Granulocyte-macrophage colony-stimulating factor ELISA Kit

E0071Bo 1 Kit
EUR 860.4

Colony Stimulating Factor 3, Granulocyte (GCSF) Polyclonal Antibody (Bovine), APC

4-PAA042Bo01-APC
  • EUR 435.60
  • EUR 4232.40
  • EUR 1170.00
  • EUR 558.00
  • EUR 271.20
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Bovine Colony Stimulating Factor 3, Granulocyte (GCSF). This antibody is labeled with APC.

Colony Stimulating Factor 3, Granulocyte (GCSF) Polyclonal Antibody (Bovine), Cy3

4-PAA042Bo01-Cy3
  • EUR 531.60
  • EUR 5593.20
  • EUR 1510.80
  • EUR 693.60
  • EUR 312.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Bovine Colony Stimulating Factor 3, Granulocyte (GCSF). This antibody is labeled with Cy3.

Colony Stimulating Factor 3, Granulocyte (GCSF) Polyclonal Antibody (Bovine), HRP

4-PAA042Bo01-HRP
  • EUR 397.20
  • EUR 3687.60
  • EUR 1034.40
  • EUR 502.80
  • EUR 255.60
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Bovine Colony Stimulating Factor 3, Granulocyte (GCSF). This antibody is labeled with HRP.

Colony Stimulating Factor 3, Granulocyte (GCSF) Polyclonal Antibody (Bovine), PE

4-PAA042Bo01-PE
  • EUR 372.00
  • EUR 3409.20
  • EUR 960.00
  • EUR 470.40
  • EUR 241.20
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Bovine Colony Stimulating Factor 3, Granulocyte (GCSF). This antibody is labeled with PE.

Colon tissue array with colon cancer tissue

BN05014a each
EUR 222
Description: Colon tissue array with colon cancer tissue, including pathology grade, TNM and clinical stage, 24 cases/72 cores, replacing BN05014

Colon cancer with matched colon tissue array

CO1801a each
EUR 474
Description: Colon cancer with matched colon tissue array, including pathology grade,with MMR (MLH1/PMS2/MSH2/MSH6) IHC results, TNM and clinical stage, containing 90 cases of adenocarcinoma, and 90 cancer adjacent or adjacent normal colon tissue, 90 cases/180 cores, replacing CO1801

Colon cancer with matched colon tissue array

CO602a each
EUR 222
Description: Colon cancer with matched colon tissue array, including pathology grade, TNM and clinical stage, 30 cases/60 cores, replacing CO602

Colon cancer with matched colon tissue array

CO703 each
EUR 198
Description: Colon cancer with matched colon tissue array, including pathology grade, TNM and clinical stage, 35 cases/70 cores,replaced by CO703a

Colony Stimulating Factor 3, Granulocyte (GCSF) Polyclonal Antibody (Bovine), FITC

4-PAA042Bo01-FITC
  • EUR 372.00
  • EUR 3409.20
  • EUR 960.00
  • EUR 470.40
  • EUR 241.20
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Bovine Colony Stimulating Factor 3, Granulocyte (GCSF). This antibody is labeled with FITC.

Colon Lysate

1472 0.1 mg
EUR 229.2
Description: Colon tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Colon Lysate

21-179 0.1 mg
EUR 342.6
Description: Monkey (Cynomolgus) colon tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The monkey (Cynomolgus) colon tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the colon tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The colon tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.

Colon Lysate

21-288 0.1 mg
EUR 342.6
Description: Monkey (Rhesus) colon tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The monkey (Rhesus) colon tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the colon tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The colon tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.

Colon carcinoma

CO1005a each
EUR 306
Description: Colon carcinoma, metastatic carcinoma and colon adenoma tissue array with colon tissue as control, including pathology grade, TNM and clinical stage (reference AJCC 8th version), 98 cases/100 cores, replacing CO1005

Colon adenocarcinoma tissue array with colon tissue

CO1201 each
EUR 348
Description: Colon adenocarcinoma tissue array with colon tissue, with pathology grade, TNM/Stage, 120 cases/120 cores (core size 1.0mm), replacing BC051110c

Colony Stimulating Factor 3, Granulocyte (GCSF) Polyclonal Antibody (Bovine), APC-Cy7

4-PAA042Bo01-APC-Cy7
  • EUR 728.40
  • EUR 8320.80
  • EUR 2197.20
  • EUR 972.00
  • EUR 399.60
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Bovine Colony Stimulating Factor 3, Granulocyte (GCSF). This antibody is labeled with APC-Cy7.

Colon cancer tissue array with normal colon tissue

T054c each
EUR 48
Description: Colon cancer tissue array with normal colon tissue, including TNM, clinical stage and pathology grade, 12 cases/ 24 cores, replaced by T054d

High-density colon cancer with colon tissue array

CO6161 each
EUR 954
Description: High-density colon cancer with colon tissue array, including pathology grade, TNM and clinical stage, 322 cases/616 cores

cDNA - Plant Normal Tissue: cDNA - Plant: Corn

C1634330 40 reactions
EUR 674

cDNA - Plant Normal Tissue: cDNA - Plant: Rice

C1634360 40 reactions
EUR 674

Colon cancer with adjacent normal colon tissue array

CO2001a each
EUR 474
Description: Colon cancer with adjacent normal colon tissue array, including TNM and pathology grade with MMR (MLH1/PMS2/MSH2/MSH6) IHC results, containing 140 cases of adenocarcinoma, 52 cases of mucinous adenocarcinoma and 8 adjacent normal colon tissue, 200 cases/ 200 cores, replacing CO2001

Colon cancer with adjacent normal colon tissue array

CO806 each
EUR 270
Description: Colon cancer with adjacent normal colon tissue array, replacing BN05012, 80 cases/80 cores

cDNA - Plant Normal Tissue: cDNA - Plant: Wheat

C1634390 40 reactions
EUR 674

Colon cancer tissue array with normal colon tissue and adjacent colon tissue as control

CO20811a each
EUR 546
Description: Colon cancer tissue array with normal colon tissue and adjacent colon tissue as control, including TNM and pathology grade with MMR (MLH1/PMS2/MSH2/MSH6) IHC results,208 cases/ 208 cores, replacing CO20811

Colony Stimulating Factor 3, Granulocyte (GCSF) Polyclonal Antibody (Bovine), Biotinylated

4-PAA042Bo01-Biotin
  • EUR 388.80
  • EUR 3172.80
  • EUR 927.60
  • EUR 478.80
  • EUR 268.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Bovine Colony Stimulating Factor 3, Granulocyte (GCSF). This antibody is labeled with Biotin.

cDNA - Plant Normal Tissue: cDNA - Plant: Orange

C1634340 40 reactions
EUR 674

cDNA - Plant Normal Tissue: cDNA - Plant: Potato

C1634350 40 reactions
EUR 674

Colon cancer with matched adjacent normal colon tissue

CO321a each
EUR 168
Description: Colon cancer with matched adjacent normal colon tissue, including pathology grade, TNM and clinical stage, 16 cases/32 cores, replacing CO321

cDNA - Plant Normal Tissue: cDNA - Plant: Arabidopsis

C1634310 40 reactions
EUR 674

Colon disease spectrum (colon cancer progression) tissue array

CO808 each
EUR 306
Description: Colon disease spectrum (colon cancer progression) tissue array, including pathology grade, TNM and clinical stage, 76 cases/80 cores

Colon adenocarcinoma with matched adjacent colon tissue array

CO487 each
EUR 168
Description: Colon adenocarcinoma with matched adjacent colon tissue array, including TNM and pathology grade, 16 cases/48 cores

Bovine COL11α1 ELISA Kit

EBC0349 96Tests
EUR 625.2

Bovine COL10 ELISA Kit

EBC0336 96Tests
EUR 625.2

Multiple types of colon disease with colon tissue array

CO245a each
EUR 66
Description: Multiple types of colon disease with colon tissue array, including crohn‘s disease, chronic inflammation of the colonic mucosa, ulcerative colitis, adenocarcinoma, 12 cases/24 cores (core size 1.5mm), replacing CO245

Different stages of colon cancer and colon tissue array

CO807 each
EUR 306
Description: Different stages of colon cancer and colon tissue array, including pathology grade, TNM and clinical satge, 80 cases/80 cores

Bovine COL1 ELISA Kit

EBC0021 96Tests
EUR 625.2

Bovine COL3 ELISA Kit

EBC0024 96Tests
EUR 625.2

Bovine COL4 ELISA Kit

EBC0054 96Tests
EUR 625.2

Bovine COL7 ELISA Kit

EBC0333 96Tests
EUR 625.2

Bovine COL2 ELISA Kit

EBC0786 96Tests
EUR 625.2

Progression of colon disease (IBD) with colon tissue array

CO246 each
EUR 66
Description: Progression of colon disease (IBD) with colon tissue array, 5 cases of crohn's disease, 2 ulcerative colitis, 5 normal colon tissue, 12 cases/24 cores

cDNA - Plant Normal Tissue: cDNA - Plant: Soy bean

C1634370 40 reactions
EUR 674

High-density colon cancer and normal colon tissue array

CO208a each
EUR 546
Description: High-density colon cancer and normal colon tissue array, 64 cases/192 cores, including TNM and pathology grade, 64 cases/192 cores (core size 1.0mm), replaced by CO208b

Colon cancer with colon tissue array (2010 WHO classification)

CO701a each
EUR 306
Description: Colon cancer with colon tissue array (2010 WHO classification), including pathology grade, TNM and clinical satge (AJCC 7th edition), 70 cases/70 cores, replacing CO701

Colon Lupus Lysate

XBL-10335 0.1 mg
EUR 796.2
Description: Human colon tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The human colon tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the colon tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The colon tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.

Fetal Colon Lysate

XBL-10403 0.1 mg
EUR 342.6
Description: Fetal human colon tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The fetal human colon tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the colon tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The colon tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.

Colon cancer tissue array with adjacent normal colon tissue

CO241a each
EUR 66
Description: Colon cancer tissue array with adjacent normal colon tissue, including TNM, clinical stage and pathology grade, 6 cases/24 cores

Bovine COL1a2 ELISA Kit

EBC0329 96Tests
EUR 625.2

Bovine COL2a1 ELISA Kit

EBC0330 96Tests
EUR 625.2

Bovine COL3a1 ELISA Kit

EBC0331 96Tests
EUR 625.2

Bovine COL6a1 ELISA Kit

EBC0332 96Tests
EUR 625.2

Bovine COL8a1 ELISA Kit

EBC0334 96Tests
EUR 625.2

Bovine COL9a1 ELISA Kit

EBC0337 96Tests
EUR 625.2

Bovine anti-Granulocyte-Macrophage Colony Stimulating Factor antibody(GM-CSF Ab)ELISA Kit

QY-E60012 96T
EUR 511.2

Bovine COL4A3BP ELISA KIT

ELI-49322b 96 Tests
EUR 1113.6

Colon Membrane Lysate

XBL-10505 0.1 mg
EUR 619.8
Description: Human colon tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human colon tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated colon tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated colon tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.

Bovine ColIV ELISA Kit

EBC0787 96Tests
EUR 625.2

Normal colon tissue array with colon cancer tissue as control

CO727 each
EUR 270
Description: Normal colon tissue array with colon cancer tissue as control, including TNM, clinical stage and pathology grade, 24 cases/ 72 cores

Colon adenocarcinoma TMA

CO571 each
EUR 270
Description: Colon adenocarcinoma TMA, 5 samples have matched cancer adjacent tissue samples, including pathology grade, TNM and clinical stage (reference AJCC 8th version) with MMR (MLH1/PMS2/MSH2/MSH6) IHC results, 52 cases/ 57 cores (core size 1.5mm) Recent Year Collection

Colon Cytoplasmic Lysate

XBL-10504 0.1 mg
EUR 273.3
Description: Human colon tissue cytoplasmic protein lysate was prepared by isolating the cytoplasmic protein from whole tissue homogenates using a proprietary technique. The human colon tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The cytoplasmic protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, glycerol, and a cocktail of protease inhibitors. For quality control purposes, the isolated colon tissue cytoplasmic protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated colon tissue cytoplasmic protein is then Western analyzed by GAPDH antibody, and the expression level is consistent with each lot.

Type 1 Bovine Collagen

TBC320 20ml @ 3mg/ml
EUR 418.8

Novo? cDNA Kit

M1165-100 each
EUR 424.8

Novo? cDNA Kit

M1165-25 each
EUR 320.4

Bovine COLXVII ELISA Kit

EBC0335 96Tests
EUR 625.2

functional genomics in the nodules of legume plant transporter

Over the last few decades, a combination of physiology, biochemistry, molecular and cell biology, and genetics has given us a basic understanding of some of the major transportation in the workplace in nodules legume nitrogen, especially those involved in the exchange of nutrients between the infected cells of plants and rhizobia endosymbiotic them.

However, our knowledge in this area remains uneven and scattered in many species of legume. Recent progress in the field of genomics and functional genomics of two nuts Model, Medicago truncatula and Lotus japonicus quickly fill the gaps in knowledge about the plant transporter gene is expressed constitutively in the nodules and other organs, and induced or otherwise specified in the nodule.

The last class in particular is the focus of current efforts to understand the special role of the nodule-specific transporter. This short article reviews past work on the biochemistry and molecular biology of plant transporter in nodules, before describing recent work in the field of transcriptomics and bioinformatics. Finally, we consider the functional genomics where together with a more classical approach tends to lead us in this area of ​​research in the future.

Bladder cancer with bladder tissue array

BL2081C each
EUR 545
Description: Bladder cancer with bladder tissue array, including pathology grade, TNM and clinical stage, 191 cases/192 cores, replacing BL2081b

Bladder cancer with bladder tissue array

BL243a each
EUR 78
Description: Bladder cancer with bladder tissue array, including urothelial carcinoma,squamous cell carcinoma, adenocarcinoma and normal tissue, pathololy grade, TNM and clinical stage (AJCC 8.0), 12 case/24 cores (1.5mm), replacing BL243

Bladder cancer with bladder tissue array

BL481d each
EUR 198
Description: Bladder cancer with bladder tissue array, including urothelial carcinoma, squamous cell carcinoma, adenocarcinoma and normal tissue, TNM/Stage, 48 cases/48 cores (core size 2.0 mm), replacing BL481d

Bladder cancer with bladder tissue array

BL807 each
EUR 306
Description: Bladder cancer with bladder tissue array, including TNM and clinical stage, 80 cases/80 cores

Bladder cancer with bladder tissue array

T124c each
EUR 78
Description: Bladder cancer with bladder tissue array, including pathology grade, TNM and clinical stage, 12 cases/24 cores, replacing T124b

Rat Bladder PrimaCell2: Normal Bladder Fibroblasts

2-82507 1 Kit Ask for price

Bladder cancer with bladder tissue test array

T121b each
EUR 66
Description: Bladder cancer with bladder tissue test array, including pathology grade, TNM and clinical stage, 6 cases/24 cores, replacing T121a

Mouse Bladder PrimaCell2: Normal Bladder Fibroblasts

2-82007 1 Kit Ask for price

Human Bladder PrimaCell2: Normal Bladder Fibroblasts

2-96008 1 Kit Ask for price

Multiple bladder carcinoma with bladder tissue array

BL1501a each
EUR 258
Description: Multiple bladder carcinoma with bladder tissue array, including pathology grade, TNM and clinical stage, 75 cases/150 cores, replacing BL1501

Rat Bladder PrimaCell1: Normal Bladder Epithelial Cells

2-82506 1 Kit Ask for price

Bladder Lysate

1410 0.1 mg
EUR 229.2
Description: Bladder tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Bladder Lysate

1478 0.1 mg
EUR 229.2
Description: Bladder tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Mouse Bladder PrimaCell1: Normal Bladder Epithelial Cells

2-82006 1 Kit Ask for price

Human Bladder PrimaCell1: Normal Bladder Epithelial Cells

2-96007 1 Kit Ask for price

Bladder cancer tissue array with normal bladder tissue

BL481b each
EUR 198
Description: Bladder cancer tissue array with normal bladder tissue, including pathology grade, TNM and clinical stage, 48 cases/48 cores, replaced by BL481c

Human Bladder PrimaCell3: Normal Bladder Smooth Muscle Cells

2-96009 1 Kit Ask for price

Rat Bladder PrimaCell2: Normal Bladder Fibroblasts Growth Medium

9-25007 5 x 100 ml Ask for price

Mouse Bladder PrimaCell2: Normal Bladder Fibroblasts Growth Medium

9-32007 5 x 100 ml Ask for price

Human Bladder PrimaCell2: Normal Bladder Fibroblasts Growth Medium

9-46008 5 x 100 ml Ask for price

Bladder cancer with bladder tissue array (2016 WHO classification)

BL208a each
EUR 546
Description: Bladder cancer with bladder tissue array (2016 WHO classification), including pathology grade, TNM and clinical stage (AJCC 7th edition), 64 cases/192 cores, replacing BL208

Rat Bladder Tissue Preparation Buffer 2: Normal Bladder Fibroblasts

9-80214 1 x 100 ml Ask for price

Rat Bladder PrimaCell1: Normal Bladder Epithelial Cells Growth Medium

9-25006 5 x 100 ml Ask for price

Human Bladder Tissue Preparation Buffer 2: Normal Bladder Fibroblasts

9-80008 1 x 100 ml Ask for price

Mouse Bladder Tissue Preparation Buffer 2: Normal Bladder Fibroblasts

9-80121 1 x 100 ml Ask for price

Mouse Bladder PrimaCell1: Normal Bladder Epithelial Cells Growth Medium

9-32006 5 x 100 ml Ask for price

Human Bladder PrimaCell1: Normal Bladder Epithelial Cells Growth Medium

9-46007 5 x 100 ml Ask for price

Bladder invasive urothelial carcinoma with matched bladder tissue array

BL244b each
EUR 66
Description: Bladder invasive urothelial carcinoma with matched bladder tissue array, including pathology grade, TNM and clinical stage, 12 cases/24 cores (1.5mm), replacing BL244a

Bladder Cancer Exosome

P141-BL - Ask for price

Bladder Membrane Lysate

XBL-10475 0.1 mg
EUR 619.8
Description: Human bladder tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human bladder tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated bladder tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated bladder tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.

Rat Bladder Tissue Preparation Buffer 1: Normal Bladder Epithelial Cells

9-80213 1 x 100 ml Ask for price

Bladder cancer tissue array with matched adjacent normal bladder tissue

BL631 each
EUR 306
Description: Bladder cancer tissue array with matched adjacent normal bladder tissue, including TNM and pathology grade, 24 cases/63 cores

Human Bladder PrimaCell3: Normal Bladder Smooth Muscle Cells Growth Medium

9-46009 5 x 100 ml Ask for price

Human Bladder Tissue Preparation Buffer 1: Normal Bladder Epithelial Cells

9-80007 1 x 100 ml Ask for price

Mouse Bladder Tissue Preparation Buffer 1: Normal Bladder Epithelial Cells

9-80120 1 x 100 ml Ask for price

Bladder urothelial carcinoma with matched cancer adjacent bladder tissue array

BL241b each
EUR 66
Description: Bladder urothelial carcinoma with matched cancer adjacent bladder tissue array, including TNM and clinical stage, 10 cases/24 cores, replacing BL241a

Human Bladder Tissue Preparation Buffer 3: Normal Bladder Smooth Muscle Cells

9-80009 1 x 100 ml Ask for price

Bladder disease spectrum (urocystic cancer progression) with bladder tissue array

BL804 each
EUR 306
Description: Bladder disease spectrum (urocystic cancer progression) with bladder tissue array, including pathology grade, TNM and clinical stage, 80 cases/80 cores

Bladder Tumor Tissue Array - 12 cases of bladder tumor/adjacent normal pairs

Z7020105 5 slides
EUR 1657.2
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Bladder Cancer Exosome RNA

P241-BL - Ask for price

Human Bladder Tumor lysate

HTL-1325 1 mg
EUR 927.6

Bladder Tissue Lysate (Tumor)

1722-01 0.1 mg
EUR 336.3
Description: Bladder tumor tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Bladder Tissue Lysate (Tumor)

1722-02 0.1 mg
EUR 336.3
Description: Bladder tumor tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Bladder Tissue Slide (Normal)

12-601-10um 10 um
EUR 241.8

Bladder Tissue Slide (Normal)

12-601-4um 4 um
EUR 216.6

Bladder cancer tissue array

BC12011c each
EUR 186
Description: Bladder cancer tissue array, including TNM, 63 cases/63 cores (core size 1.5mm)

Normal bladder tissue array

BNC12011 each
EUR 222
Description: Normal bladder tissue array, including TNM, clinical stage and pathology grade, 40 cases/ 80 cores

Bladder cancer tissue array

BL2082a each
EUR 546
Description: Bladder cancer tissue array, including TNM, clinical stage and pathology grade, 208 cases/ 208 cores, replacing BL2082

Bladder cancer tissue array

BL2082B each
EUR 546
Description: Bladder cancer tissue array, including TNM and clinical stage, 208 cases/208 cores, replacing BL2082a

Bladder cancer tissue array

BL242 each
EUR 270
Description: Bladder cancer tissue array, with normal tissue, including TNM and pathology grade, 24 cases/48 cores

Bladder Tissue Lysate (Normal)

1721-01 0.1 mg
EUR 260.7
Description: Bladder tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Cancer of the bladder, 102 cases (1.5mm)

BLC1021 1
EUR 354
Description: Bladder cancer tissue array, 102 cases of normal/benign (5 cases) and cancer (97 cases) tissues, with grading and TNM staging data.

Cancer of the bladder paired with normal adjacent bladder tissue, 24 cases (2mm), set 1

BLC481 1
EUR 300
Description: Bladder cancer tissue array, set 1, containing 24 cases in 48 cores, with normal paired with tumor tissues from the same patients, with grading and TNM staging data.

Rat Bladder Whole tissue lysate

RAL-1478 1 mg
EUR 628.8

Mouse Bladder Whole tissue lysate

MAL-1410 1 mg
EUR 927.6

Rat Bladder PrimaCell2: Normal Bladder Fibroblasts Growth Supplements with Serum (for 500 ml medium)

9-26007 1 Set Ask for price

Hamster IgM

31C-CH0707 1 mg
EUR 492
Description: Purified Hamster IgM

Hamster Mono Anti-Mouse CD3e IgG (Hamster IgG)

MCD003E-M 100 Tests
EUR 578.4

Hamster Mono Anti-Mouse CD3z IgG (Hamster IgG)

MCD003Z-M 100 ug
EUR 578.4

Human Bladder Cancer Primer Library

HBLCP-I 1 set
EUR 657.6

Mouse Bladder PrimaCell2: Normal Bladder Fibroblasts Growth Supplements with Serum (for 500 ml medium)

9-33007 1 Set Ask for price

Human Bladder PrimaCell2: Normal Bladder Fibroblasts Growth Supplements with Serum (for 500 ml medium)

9-47008 1 Set Ask for price

Total RNA - Rat Normal Tissue: Bladder

R1434010-50 50 ug
EUR 322

Cancer of the bladder, 75 cases (1.1mm)

BLC1501 1
EUR 354
Description: Bladder cancer tissue array, 150 cores from normal, inflammatory (5 cases) and cancer (70 cases) tissues in duplicates, with grading and TNM staging data.

Cancer of the bladder, 33 cases (1.5mm)

BLC661 1
EUR 300
Description: Bladder cancer tissue array, 66 cores including bladder cancers (29 cases) and uninvolved bladder tissues (4 cases) in duplicates, with grading and TNM staging data.

Rat Bladder PrimaCell1: Normal Bladder Epithelial Cells Growth Supplements with Serum (for 500 ml medium)

9-26006 1 Set Ask for price

Mouse Bladder PrimaCell1: Normal Bladder Epithelial Cells Growth Supplements with Serum (for 500 ml medium)

9-33006 1 Set Ask for price

Human Bladder PrimaCell1: Normal Bladder Epithelial Cells Growth Supplements with Serum (for 500 ml medium)

9-47007 1 Set Ask for price

Total RNA - Human Tumor Tissue: Bladder

R1235010-10 10 ug
EUR 400

Human Bladder tumor antigen ELISA Kit

ELA-E0637h 96 Tests
EUR 988.8

Total RNA - Mouse Normal Tissue: Bladder

R1334010-50 50 ug
EUR 322

Bladder Tissue Slide (papillary Carcinoma)

12-612-10um 10 um
EUR 241.8

Bladder Tissue Slide (papillary Carcinoma)

12-612-4um 4 um
EUR 216.6

Bladder cancer and normal tissue array

BL1002b each
EUR 306
Description: Bladder cancer and normal tissue array, including pathology grade, TNM and clinical stage, 50 cases/ 100 cores, replacing BL1002a

Human Bladder PrimaCell3: Normal Bladder Smooth Muscle Cells Growth Supplements with Serum (for 500 ml medium)

9-47009 1 Set Ask for price

Rat Bladder Tumor Antigen (BTA) CLIA Kit

abx196754-96tests 96 tests
EUR 990

Total Protein - Human Tumor Tissue: Bladder

P1235010 1 mg
EUR 310

Hamster IgG (HRP)

65C-CH0703 1 mg
EUR 296.4
Description: Purified Hamster IgG HRP conjugate

Rat Bladder Tumor Antigen (BTA) ELISA Kit

abx256747-96tests 96 tests
EUR 848.4

Rat Bladder tumor antigen,BTA ELISA kit

E02B0860-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Rat Bladder tumor antigen,BTA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Bladder tumor antigen,BTA ELISA kit

E02B0860-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Rat Bladder tumor antigen,BTA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Bladder tumor antigen,BTA ELISA kit

E02B0860-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Rat Bladder tumor antigen,BTA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Bladder Tumor Antigen (BTA) ELISA Kit

abx360928-96tests 96 tests
EUR 990

Rat Bladder tumor antigen,BTA ELISA Kit

CN-01694R1 96T
EUR 537.6

Rat Bladder tumor antigen,BTA ELISA Kit

CN-01694R2 48T
EUR 356.4

Dog Bladder tumor antigen,BTA ELISA kit

E08B0860-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Canine Bladder tumor antigen,BTA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Bladder tumor antigen,BTA ELISA kit

E08B0860-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Canine Bladder tumor antigen,BTA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Bladder tumor antigen,BTA ELISA kit

E08B0860-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Canine Bladder tumor antigen,BTA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Bladder tumor antigen,BTA ELISA kit

E07B0860-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Porcine Bladder tumor antigen,BTA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Bladder tumor antigen,BTA ELISA kit

E07B0860-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Porcine Bladder tumor antigen,BTA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Bladder tumor antigen,BTA ELISA kit

E07B0860-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Porcine Bladder tumor antigen,BTA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Bladder tumor antigen(BTA)ELISA Kit

GA-E0156RT-48T 48T
EUR 447.6

Rat Bladder tumor antigen(BTA)ELISA Kit

GA-E0156RT-96T 96T
EUR 730.8

Human Bladder Slide (Normal) (5 slides/pk)

HTS-12601 1 pk
EUR 343.2

Rat Bladder tumor antigen(BTA)ELISA Kit

QY-E11496 96T
EUR 433.2

Rat Bladder tumor antigen,BTA ELISA Kit

YLA0838RA-48T 48T
EUR 465

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