Diversity, enrichment, and genomic potential of anaerobic methane- and ammonium

Diversity, enrichment, and genomic potential of anaerobic methane- and ammonium-oxidizing microorganisms from a brewery wastewater treatment plant

 

 

Genomic potential of anaerobic methane- and ammonium

Anaerobic wastewater treatment offers several advantages; However, waste from anaerobic digester still contains high levels of dissolved ammonium and methane that need to be removed before the waste can be discharged into surface water. Elimination of simultaneous anaerobic methane and ammonium by denitrification (N-Damo) methanotrophs in combination with the anaerobic ammonium-oxidizing (Anammox) bacteria could be a potential solution to this challenge. After surveying the molecules of waste brewery waste water treating plants, which indicates the presence of both N-Damo and bacteria Anammox, we started an anaerobic bioreactor with a continuous supply of methane, ammonia and nitrite to enrich this anaerobic microorganisms.

After 14 months of operation, stable enrichment cultures containing two types of ‘Candidatus Methylomirabilis oxyfera’ bacteria and two strains of ‘Ca. Brocadia’-like bacteria Anammox achieved. In this community, the bacteria Anammox converted 80% of the ammonium nitrite, while ‘Ca. Methylomirabilis’ contribute 20% of the consumption of nitrite. Analysis of 16S rRNA metagenomic reads and fluorescence in situ hybridization (FISH) correlate well and show that, after 14 months, ‘Ca. Methylomirabilis’ and Anammox bacteria is about 30 and 20% of the total microbial community. In addition, the majority (10%) of society consists of Planctomycetes Phycisphaera related.

Assembly and binning of metagenomic sequence resulting in a high-quality draft genome of two ‘Ca. Methylomirabilis species’ contains a marker gene pmoCAB, xoxF, and NIR and allegedly NO dismutase gene. The Anammox draft genome is closely related to ‘Ca. Brocadia Fulgida ‘including hzsABC marker gene, hao, and HDH. Whole-batch anaerobic reactor and measuring the activity of methane, ammonia, nitrite, and nitrate revealed the average anaerobic methane oxidation rate of 0.12 mmol h-1 L-1 and ammonium oxidation rate of 0.5 mmol h-1 L-1.

Together, these studies illustrate the draft genome enrichment and anaerobic methanotrophs of brewery wastewater treatment, where this organism together with Anammox bacteria can contribute significantly to the elimination of methane and ammonium in a more sustainable way. KEY POINTS: • An enrichment culture containing N-Damo and bacteria Anammox obtained. • Simultaneous consumption of ammonia, nitrite, and methane under anoxic conditions. • In-depth analysis of metagenomic biodiversity inoculum and cultural enrichment.

 Diversity, enrichment, and genomic potential of anaerobic methane- and ammonium-oxidizing microorganisms from a brewery wastewater treatment plant
Diversity, enrichment, and genomic potential of anaerobic methane- and ammonium-oxidizing microorganisms from a brewery wastewater treatment plant



Equine Small Intestine Paraffin Sections

EP-306 10 slides
EUR 240

Chicken Small Intestine cDNA

CD-306 30 reactions
EUR 243

cDNA - Lupus: Small Intestine

C1236226Lup 40 reactions
EUR 811

Cat Small Intestine, Ileum cDNA

FD-309 30 Reactions
EUR 280

Dog Small Intestine, Ileum cDNA

DD-309 30 Reactions
EUR 280

Pig Small Intestine, Ileum cDNA

PD-309 30 reactions
EUR 243

Cat Small Intestine, Jejunum cDNA

FD-308 30 Reactions
EUR 280

Dog Small Intestine, Jejunum cDNA

DD-308 30 Reactions
EUR 280

Pig Small Intestine, Jejunum cDNA

PD-308 30 reactions
EUR 243

Sheep Small Intestine, Ileum cDNA

SD-309 30 reactions
EUR 243

Cat Small Intestine, Duodenum cDNA

FD-307 30 Reactions
EUR 280

Dog Small Intestine, Duodenum cDNA

DD-307 30 Reactions
EUR 280

Bovine Small Intestine, Ileum cDNA

BD-309 30 reactions
EUR 243

Rabbit Small Intestine, Ileum cDNA

TD-309 30 reactions
EUR 243

Pig Small Intestine, Duodenum cDNA

PD-307 30 reactions
EUR 243

Hamster Small Intestine, Ileum cDNA

AD-309 30 reactions
EUR 243

MiniPig Small Intestine, Ileum cDNA

ND-309 30 reactions
EUR 358

Sheep Small Intestine, Jejunum cDNA

SD-308 30 reactions
EUR 243

Human Small Intestine cDNA-Oligo-dT

HD-306 30 reactions
EUR 280

Bovine Small Intestine, Jejunum cDNA

BD-308 30 reactions
EUR 243

Rabbit Small Intestine, Jejunum cDNA

TD-308 30 reactions
EUR 243

Sheep Small Intestine, Duodenum cDNA

SD-307 30 reactions
EUR 243

Hamster Small Intestine, Jejunum cDNA

AD-308 30 reactions
EUR 243

Bovine Small Intestine, Duodenum cDNA

BD-307 30 reactions
EUR 243

cDNA - Liver Cirrhosis: Small Intestine

C1236226Lcs 40 reactions
EUR 802

Rabbit Small Intestine, Duodenum cDNA

TD-307 30 reactions
EUR 243

MiniPig Small Intestine, Jejunum cDNA

ND-308 30 reactions
EUR 358

Hamster Small Intestine, Duodenum cDNA

AD-307 30 reactions
EUR 243

MiniPig Small Intestine, Duodenum cDNA

ND-307 30 reactions
EUR 358

Human Small Intestine cDNA-Random Primer

HD-306-HR 30 reactions
EUR 280

Guinea Pig Small Intestine, Ileum cDNA

GD-309 30 reactions
EUR 243

cDNA - Rat Normal Tissue: Small Intestine

C1434226 40 reactions
EUR 636

cDNA - Dog Normal Tissue: Small Intestine

C1734226 40 reactions
EUR 424

Guinea Pig Small Intestine, Jejunum cDNA

GD-308 30 reactions
EUR 243

cDNA - Mouse Normal Tissue: Small Intestine

C1334226 40 reactions
EUR 636

Guinea Pig Small Intestine, Duodenum cDNA

GD-307 30 reactions
EUR 243

Monkey Small Intestine, Ileum cDNA, Rhesus

UD-309 30 reactions
EUR 316

cDNA - Liver Cirrhosis: Small Intestine: Ileum

C1236227Lcs 40 reactions
EUR 802

Monkey Small Intestine, Jejunum cDNA, Rhesus

UD-308 30 reactions
EUR 316

Monkey Small Intestine, Duodenum cDNA, Rhesus

UD-307 30 reactions
EUR 316

Equine Intestine Genomic DNA

GE-306 0.1mg
EUR 210

cDNA - Liver Cirrhosis: Small Intestine: Jejunum

C1236230Lcs 40 reactions
EUR 802

Monkey Small Intestine, Ileum cDNA, Cynomolgus

KD-309 30 reactions
EUR 316

cDNA - Liver Cirrhosis: Small Intestine: Duodenum

C1236101Lcs 40 reactions
EUR 802

Monkey Small Intestine, Jejunum cDNA, Cynomolgus

KD-308 30 reactions
EUR 316

Monkey Small Intestine, Duodenum cDNA, Cynomolgus

KD-307 30 reactions
EUR 316

cDNA - Human Adult Normal Tissue: Small Intestine

C1234226 40 reactions
EUR 424

cDNA - Monkey (Rhesus) Normal Tissue: Small Intestine

C1534226 40 reactions
EUR 723

cDNA - Monkey (Cynomolgus) Normal Tissue: Small Intestine

C1534226-Cy 40 reactions
EUR 723

cDNA - Human Adult Normal Tissue: Small Intestine: Ileum

C1234227 40 reactions
EUR 424

cDNA - Human Adult Normal Tissue: Small Intestine: Jejunum

C1234230 40 reactions
EUR 424

cDNA - Monkey (Rhesus) Normal Tissue: Small Intestine: Ileum

C1534227 40 reactions
EUR 723

cDNA - Human Adult Normal Tissue: Small Intestine: Duodenum

C1234101-10 10 reactions
EUR 237

cDNA - Monkey (Rhesus) Normal Tissue: Small Intestine: Jejunum

C1534230 40 reactions
EUR 723

Monkey Small Intestine, Ileum cDNA-Random Primer, Rhesus

UD-309-RH 30 reactions
EUR 316

cDNA - Human Diabetic Diseased Tissue: Small Intestine: Ileum

C1236227Dia 40 reactions
EUR 802

cDNA - Monkey (Rhesus) Normal Tissue: Small Intestine: Duodenum

C1534101 40 reactions
EUR 723

cDNA - Monkey (Cynomolgus) Normal Tissue: Small Intestine: Ileum

C1534227-Cy 40 reactions
EUR 723

Monkey Small Intestine, Jejunum cDNA-Random Primer, Rhesus

UD-308-RH 30 reactions
EUR 316

cDNA - Human Diabetic Diseased Tissue: Small Intestine: Jejunum

C1236230Dia 40 reactions
EUR 802

Monkey Small Intestine, Duodenum cDNA-Random Primer, Rhesus

UD-307-RH 30 reactions
EUR 316

cDNA - Human Diabetic Diseased Tissue: Small Intestine: Duodenum

C1236101Dia 40 reactions
EUR 802

cDNA - Monkey (Cynomolgus) Normal Tissue: Small Intestine: Jejunum

C1534230-Cy 40 reactions
EUR 723

Monkey Small Intestine, Ileum cDNA-Random Primer, Cynomolgus

KD-309-RH 30 reactions
EUR 316

cDNA - Monkey (Cynomolgus) Normal Tissue: Small Intestine: Duodenum

C1534101-Cy 40 reactions
EUR 723

Monkey Small Intestine, Jejunum cDNA-Random Primer, Cynomolgus

KD-308-RH 30 reactions
EUR 316

Monkey Small Intestine, Duodenum cDNA-Random Primer, Cynomolgus

KD-307-RH 30 reactions
EUR 316

Small Intestine Lysate

1308 0.1 mg
EUR 229.2
Description: Small intestine tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Small Intestine Lysate

1468 0.1 mg
EUR 229.2
Description: Small Intestine tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Small intestine cancer

SM208 each
EUR 546
Description: Small intestine cancer, metastatic lymph nodes and normal tissue high density tissue microarray, 69 cases/208 core, with stage and grade data

Small intestine disease spectrum (small intestine cancer progression) tissue array

SM802 each
EUR 306
Description: Small intestine disease spectrum (small intestine cancer progression) tissue array, with grade, stage and TNM data, 72 cases/80 cores

Small Intestine Tumor Lysate

1338 0.1 mg
EUR 304.8
Description: Small Intestine tumor tissue lysate was prepared by homogenization in lysis buffer (10 mM HEPES pH7.9, 1.5 mM MgCl2, 10 mM KCl, 1 mM ethylenediaminetetraacetic acid, 10% glycerol, 1% NP-40, and a cocktail of protease inhibitors). Tissue and cell debris was removed by centrifugation. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

small intestine Tissue block

31 1 unit
EUR 435

Small Intestine Membrane Lysate

XBL-10876 0.1 mg
EUR 619.8
Description: Human small intestine tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human small intestine tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated small intestine tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated small intestine tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.

Chicken Small Intestine Sections

CP-306 10 slides
EUR 240

Small Intestine Cytoplasmic Lysate

XBL-10875 0.1 mg
EUR 273.3
Description: Human small intestine tissue cytoplasmic protein lysate was prepared by isolating the cytoplasmic protein from whole tissue homogenates using a proprietary technique. The human small intestine tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The cytoplasmic protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, glycerol, and a cocktail of protease inhibitors. For quality control purposes, the isolated small intestine tissue cytoplasmic protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated small intestine tissue cytoplasmic protein is then Western analyzed by GAPDH antibody, and the expression level is consistent with each lot.

Dog Small Intestine, Jejunum RNA

DR-308 0.1mg
EUR 195

Human Small Intestine Total RNA

HR-306 0.05mg
EUR 172

Total RNA - Lupus: Small Intestine

R1236226Lup-50 50 ug
EUR 460

Dog Small Intestine, Duodenum RNA

DR-307 0.1mg
EUR 195

Chicken Small Intestine Total RNA

CR-306 0.1mg
EUR 160

Rat Small Intestine Tissue Lysate

IRTINTSTL100UG each
EUR 245
Description: Rat Small Intestine Tissue Lysate

Human Small Intestine Tumor lysate

HTL-1338 1 mg
EUR 927.6

Small intestine Tissue Slide (Normal)

11-801-10um 10 um
EUR 241.8

Small intestine Tissue Slide (Normal)

11-801-4um 4 um
EUR 216.6

Normal small intestine tissue array

SMN601 each
EUR 222
Description: Normal small intestine tissue array, including TNM, clinical stage and pathology grade, 30 cases/ 60 cores

Mouse Small Intestine Tissue Lysate

IMSINTSTL100UG each
EUR 245
Description: Mouse Small Intestine Tissue Lysate

Human Small Intestine Total Protein

HT-306 1mg
EUR 176

Small Intestine Tissue Slide (Abnormal)

11-824-10um 10 um
EUR 241.8

Small Intestine Tissue Slide (Abnormal)

11-824-4um 4 um
EUR 216.6

Chicken Small Intestine Total Protein

CT-306 1mg
EUR 153

Small Intestine Membrane Tumor Lysate

XBL-10888 0.1 mg
EUR 752.1
Description: Human small intestine tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human small intestine tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated small intestine tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated small intestine tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.

Small Intestine Tissue Slide (Malignant)

11-814-10um 10 um
EUR 241.8

Small Intestine Tissue Slide (Malignant)

11-814-4um 4 um
EUR 216.6

Human Small Intestine Epithelial Cells

ABC-TC3814 1 vial Ask for price
Description: Human small intestines cells are derived from whole small intestines that have been dissociated into multi-cellular aggregates and single cells then frozen. Human whole small intestines cells are from a single donor. The small intestine consists of several specialized epithelial cells that could help researchers determine the absorption potential of drug candidates. In addition, these cells can be used with studies of transport and metabolism of drugs and dietary chemicals.

Small Intestine Liver Cirrhosis Lysate

XBL-10377 0.1 mg
EUR 796.2
Description: Human small intestine tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The human small intestine tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the small intestine tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The small intestine tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.

copper nanowires as nanofertilizers to plant alfalfa: Understanding the interaction of nano-bio systems of microbial genomics, plant molecular response and spectroscopic studies

The latest applications of nano copper (Cu) in agrosystem compounds have demonstrated the potential to improve the performance of plant physiological and agronomic parameters. We grew alfalfa (Medicago sativa) in potting mix changed with bulk, nano, and Cu ionic compound at 80 and 280 mg Cu / kg; then, we evaluate the performance of the plant at the physiological and molecular level.

Plants treated with the precipitation / Cu nano served better agronomic response. P and S content is reduced in bulk and ion Cu-exposed plants, compared with controls (p ≤ 0.05). All forms of Cu increase the content of Fe and Zn in the Fe in the roots and leaves, compared to controls (p ≤ 0.05).

Leaf-superoxide dismutase expression was plus ~ 27-fold and Rubisco mRNA was not affected in bulk / nano Cu-treated plants, compared with controls (p ≤ 0.05). Bulk / nano Cu increased relative abundance of microorganisms involved in uptake. These results indicate that the nano Cu improve the physiology of alfalfa and could be considered as a potential nanofertilizers.

MiniPig Kidney cDNA

ND-901 30 reactions
EUR 358

cDNA - Lupus: Kidney

C1236142Lup 40 reactions
EUR 811

Mouse CD1 Kidney cDNA

MD-901 30 reactions
EUR 243

Mouse C57 Kidney cDNA

MD-901-C57 30 reactions
EUR 280

Guinea Pig Kidney cDNA

GD-901 30 reactions
EUR 243

Monkey Kidney cDNA, Rhesus

UD-901 30 reactions
EUR 316

Monkey Kidney cDNA, Cynomolgus

KD-901 30 reactions
EUR 316

cDNA - hypertension: Kidney

C1236142Hd-2 40 reactions
EUR 802

Cat Kidney Total RNA

FR-901 0.1mg
EUR 195

Cat Kidney Genomic DNA

FG-901 0.1mg
EUR 210

Cat Kidney Total Protein

FT-901 1mg
EUR 176

Human Kidney cDNA-Oligo-dT

HD-901 30 reactions
EUR 280

Cat Kidney Frozen Sections

FF-901 10 slides
EUR 261

Rat Kidney cDNA-Random Primer

RD-901-RH 30 reactions
EUR 243

Cat Kidney Paraffin Sections

FP-901 10 slides
EUR 240

Human Kidney cDNA-Random Primer

HD-901-HR 30 reactions
EUR 280

cDNA - Liver Cirrhosis: Kidney

C1236142Lcs 40 reactions
EUR 802

Rat WS Kidney cDNA-Oligo-dT

RD-901-WS 30 reactions
EUR 243

CRFK Cells – Cat Kidney Cortex 

63-XXXX each Ask for price

cDNA - Dog Normal Tissue: Kidney

C1734142 40 reactions
EUR 424

cDNA - Rat Normal Tissue: Kidney

C1434142 40 reactions
EUR 424

Mouse C57 Kidney cDNA-Random Primer

MD-901-C57-RH 30 reactions
EUR 280

Mouse CD1 Kidney cDNA-Random Primer

MD-901-HR 30 reactions
EUR 243

cDNA - Human Tumor Tissue: Kidney

C1235142 40 reactions
EUR 636

cDNA - Mouse Normal Tissue: Kidney

C1334142 40 reactions
EUR 424

Monkey Kidney cDNA-Random Primer, Rhesus

UD-901-RH 30 reactions
EUR 316

Monkey Kidney cDNA-Random Primer, Cynomolgus

KD-901-RH 30 reactions
EUR 316

cDNA - Human Adult Normal Tissue: Kidney

C1234142 40 reactions
EUR 424

cDNA - Monkey (Rhesus) Normal Tissue: Kidney

C1534142 40 reactions
EUR 481

cDNA - Human Diabetic Diseased Tissue: Kidney

C1236142Dia 40 reactions
EUR 802

cDNA - Monkey (Cynomolgus) Normal Tissue: Kidney

C1534142-Cy 40 reactions
EUR 481

Matched Pair - cDNA - Human Primary Tumor and Normal Tissue: Kidney

C8235142-PP 10 reactions x2
EUR 583

Matched Pair - cDNA - Human Primary and Matched Metastatic Tumor Tissue: Kidney

C8235142-PM 10 reactions x2
EUR 1212

Keg1 (untagged) - Mouse kidney expressed gene 1 (cDNA clone MGC:18886 IMAGE:4238654), (10ug)

MC206787 10 µg Ask for price

Multiple kidney tumor with kidney tissue array

KD1501a each
EUR 258
Description: Multiple kidney tumor with kidney tissue array, including pathology grade, TNM and clinical stage (reference AJCC 7th version), 75 cases/150 cores, replacing KD1501

Kidney cancer with matched kidney tissue array

KD801a each
EUR 258
Description: Kidney cancer with matched kidney tissue array, including pathology grade, TNM and clinical stagr, 40 cases/80 cores, replacing KD801

Kidney carcinoma with matched kidney tissue array

KD244a each
EUR 48
Description: Kidney carcinoma with matched kidney tissue array, including pathology grade, TNM and clinical stage, 12 cases/24 cores, replacing KD244

Rat Kidney PrimaCell2: Normal Kidney Epithelial Cells

2-82556 1 Kit Ask for price

Human Kidney

HS0055 6 Slides/Pack
EUR 160

Mouse Kidney PrimaCell2: Normal Kidney Epithelial Cells

2-82057 1 Kit Ask for price

Human Kidney PrimaCell2: Normal Kidney Epithelial Cells

2-96075 1 Kit Ask for price

Kidney cancer with kidney tissue test tissue array

T071b each
EUR 48
Description: Kidney cancer with kidney tissue test tissue array, including pathology grade, TNM and clinical stage, 6 cases/24 cores, replacing T071a

Kidney Lysate

1465 0.1 mg
EUR 229.2
Description: Kidney tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Kidney Lysate

21-104 0.1 mg
EUR 342.6
Description: Bovine kidney tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The bovine kidney tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the kidney tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The kidney tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.

Kidney Lysate

21-116 0.1 mg
EUR 342.6
Description: Guinea Pig kidney tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The guinea pig kidney tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the kidney tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The kidney tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.

Kidney Lysate

21-190 0.1 mg
EUR 342.6
Description: Monkey (Cynomolgus) kidney tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The monkey (Cynomolgus) kidney tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the kidney tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The kidney tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.

Kidney Lysate

21-405 0.1 mg
EUR 342.6
Description: Porcine kidney tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The porcine kidney tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the kidney tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The kidney tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.

Kidney Lysate

21-418 0.1 mg
EUR 342.6
Description: Rabbit kidney tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The rabbit kidney tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the kidney tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The kidney tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.

Kidney cancer tissue array with normal Kidney tissue

KD2082a each
EUR 474
Description: Kidney cancer tissue array with normal Kidney tissue, including TNM and pathology grade, 96 cases/192 cores, replacing KD2082

Normal kidney and normal adjacent kidney tissue array

KDN241a each
EUR 138
Description: Normal kidney and normal adjacent kidney tissue array, 24 cases/ 24 cores

Multiple kidney cancer with matched kidney tissue array

KD461 each
EUR 258
Description: Multiple kidney cancer with matched kidney tissue array, including pathology grade, TNM and clinical stage, 23 cases/46 cores

Kidney disease spectrum (kidney cancer progression) tissue array

BC07001 each
EUR 258
Description: Kidney disease spectrum (kidney cancer progression) tissue array, including TNM and pathology grade, 40 cases/80 cores (core size 1.5mm)

Dog Kidney RNA

DR-901 0.1mg
EUR 195

Kidney cancer tissue array with adjacent normal kidney tissue

BC07015b each
EUR 168
Description: Kidney cancer tissue array with adjacent normal kidney tissue, including TNM, clinical stage and pathology grade, 72 cases/72 cores, replacing BC07015a

Kidney cancer with matched adjacent normal kidney tissue array

KD603 each
EUR 204
Description: Kidney cancer with matched adjacent normal kidney tissue array, containing metastatic clear cell carcinoma, including pathology grade, TNM and clinical stage (reference AJCC 8th version), 33 cases/61 cores

Rat Kidney Cells

ABC-TC4145 1 vial Ask for price
Description: Rat primary kidney cells (RPKIDCs) are isolated from tissue of 1-day-old neonatal Sprague–Dawley rats. RPKIDCs are mixed population of mouse kidney cells grown in T75 tissue culture flasks pre-coated with 0.2% gelatin for 1 hour and incubated in Culture Complete Growth Medium.

Human Kidney CAFs

CAF110 1,000,000 cells Ask for price

Human Kidney CAFs

CAF110-IM each
EUR 9536.4

Rat Kidney PrimaCell2: Normal Kidney Epithelial Cells Growth Medium

9-25056 5 x 100 ml Ask for price

Mouse Kidney PrimaCell2: Normal Kidney Epithelial Cells Growth Medium

9-32057 5 x 100 ml Ask for price

Human Kidney PrimaCell2: Normal Kidney Epithelial Cells Growth Medium

9-46075 5 x 100 ml Ask for price

BOVINE, KIDNEY, FRESH

8600832 1EA
EUR 45.13

kidney Tissue block

11 1 unit
EUR 435

Kidney cancer (CCRCC

KD1201 each
EUR 306
Description: Kidney cancer (CCRCC,PRCC,CRCC)with kidney tissue array,including ISUP Grade, TNM/Stage, 120 cases/120 cores (core size 1.0mm ), (Previous Item# is BC07115b)

Kidney tissue array

KD803b each
EUR 354
Description: Kidney tissue array, 80 cases/80n cores (core size 1.5mm), replacing KD803a

Kidney Lupus Lysate

XBL-10357 0.1 mg
EUR 796.2
Description: Human kidney tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The human kidney tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the kidney tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The kidney tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.

Fetal Kidney Lysate

XBL-10408 0.1 mg
EUR 342.6
Description: Fetal human kidney tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The fetal human kidney tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the kidney tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The kidney tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.

PORCINE, KIDNEY, FRESH

8604932 1EA
EUR 39.28

BOVINE, KIDNEY, FROZEN

8620832 1EA
EUR 45.13

Kidney tumor tissue array with matched tumor adjacent kidney tissue

KD2602a each
EUR 354
Description: Kidney tumor tissue array with matched tumor adjacent kidney tissue, including TNM, clinical stage and pathology grade, 65 cases/260 cores, replacing KD2602

PORCINE, KIDNEY, FROZEN

8624932 1EA
EUR 39.28

Kidney Cancer Exosome

P141-KN - Ask for price

Rat Kidney Tissue Preparation Buffer 2: Normal Kidney Epithelial Cells

9-80263 1 x 100 ml Ask for price

Kidney cancer tissue array with matched cancer adjacent kidney tissue

KD2601a each
EUR 354
Description: Kidney cancer tissue array with matched cancer adjacent kidney tissue,including TNM, clinical stage and pathology grade, 65 cases/ 260 cores, replacing KD2061

Kidney clear cell carcinoma with matched adjacent kidney tissue array

KD486 each
EUR 168
Description: Kidney clear cell carcinoma with matched adjacent kidney tissue array, including TNM and pathology grade, 16 cases/48 cores

Kidney cancer tissue array with matched adjacent normal kidney tissue

KD901a each
EUR 258
Description: Kidney cancer tissue array with matched adjacent normal kidney tissue, including TNM, clinical stage and pathology grade, 40 cases/90 cores, replacing KD901

Rat Kidney Fibroblasts

ABC-TC4147 1 vial Ask for price
Description: Rat kidney fibroblasts are isolated from tissue of 1-day-old neonatal Sprague–Dawley rats. Rat kidney fibroblasts are grown in T75 tissue culture flasks pre-coated with 0.2% gelatin for 1 hour and incubated in Culture Complete Growth Medium.

Chinese Hamster Kidney

81-XXXX each Ask for price

OORA00340-10U - KIDNEY

OORA00340-10U 1Each
EUR 105

OORA00340-1EA - KIDNEY

OORA00340-1EA 1Each
EUR 99

OORA00480-10U - KIDNEY

OORA00480-10U 1Each
EUR 55

OORA00480-1EA - KIDNEY

OORA00480-1EA 1Each
EUR 59

Kidney Membrane Lysate

XBL-10649 0.1 mg
EUR 619.8
Description: Human kidney tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human kidney tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated kidney tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated kidney tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.

Human Kidney Tissue Preparation Buffer 2: Normal Kidney Epithelial Cells

9-80075 1 x 100 ml Ask for price

Mouse Kidney Tissue Preparation Buffer 2: Normal Kidney Epithelial Cells

9-80171 1 x 100 ml Ask for price

Chicken Kidney Sections

CP-901 10 slides
EUR 240

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