Diversity, enrichment, and genomic potential of anaerobic methane- and ammonium

Diversity, enrichment, and genomic potential of anaerobic methane- and ammonium-oxidizing microorganisms from a brewery wastewater treatment plant

 

 

Genomic potential of anaerobic methane- and ammonium

Anaerobic wastewater treatment offers several advantages; However, waste from anaerobic digester still contains high levels of dissolved ammonium and methane that need to be removed before the waste can be discharged into surface water. Elimination of simultaneous anaerobic methane and ammonium by denitrification (N-Damo) methanotrophs in combination with the anaerobic ammonium-oxidizing (Anammox) bacteria could be a potential solution to this challenge. After surveying the molecules of waste brewery waste water treating plants, which indicates the presence of both N-Damo and bacteria Anammox, we started an anaerobic bioreactor with a continuous supply of methane, ammonia and nitrite to enrich this anaerobic microorganisms.

After 14 months of operation, stable enrichment cultures containing two types of ‘Candidatus Methylomirabilis oxyfera’ bacteria and two strains of ‘Ca. Brocadia’-like bacteria Anammox achieved. In this community, the bacteria Anammox converted 80% of the ammonium nitrite, while ‘Ca. Methylomirabilis’ contribute 20% of the consumption of nitrite. Analysis of 16S rRNA metagenomic reads and fluorescence in situ hybridization (FISH) correlate well and show that, after 14 months, ‘Ca. Methylomirabilis’ and Anammox bacteria is about 30 and 20% of the total microbial community. In addition, the majority (10%) of society consists of Planctomycetes Phycisphaera related.

Assembly and binning of metagenomic sequence resulting in a high-quality draft genome of two ‘Ca. Methylomirabilis species’ contains a marker gene pmoCAB, xoxF, and NIR and allegedly NO dismutase gene. The Anammox draft genome is closely related to ‘Ca. Brocadia Fulgida ‘including hzsABC marker gene, hao, and HDH. Whole-batch anaerobic reactor and measuring the activity of methane, ammonia, nitrite, and nitrate revealed the average anaerobic methane oxidation rate of 0.12 mmol h-1 L-1 and ammonium oxidation rate of 0.5 mmol h-1 L-1.

Together, these studies illustrate the draft genome enrichment and anaerobic methanotrophs of brewery wastewater treatment, where this organism together with Anammox bacteria can contribute significantly to the elimination of methane and ammonium in a more sustainable way. KEY POINTS: • An enrichment culture containing N-Damo and bacteria Anammox obtained. • Simultaneous consumption of ammonia, nitrite, and methane under anoxic conditions. • In-depth analysis of metagenomic biodiversity inoculum and cultural enrichment.

 Diversity, enrichment, and genomic potential of anaerobic methane- and ammonium-oxidizing microorganisms from a brewery wastewater treatment plant
Diversity, enrichment, and genomic potential of anaerobic methane- and ammonium-oxidizing microorganisms from a brewery wastewater treatment plant



cDNA - Mouse Normal Tissue: Small Intestine

C1334226 40 reactions
EUR 578

cDNA - Liver Cirrhosis: Small Intestine: Ileum

C1236227Lcs 40 reactions
EUR 729

cDNA - Liver Cirrhosis: Small Intestine: Jejunum

C1236230Lcs 40 reactions
EUR 729

cDNA - Liver Cirrhosis: Small Intestine: Duodenum

C1236101Lcs 40 reactions
EUR 729

cDNA - Human Adult Normal Tissue: Small Intestine

C1234226 40 reactions
EUR 385

cDNA - Monkey (Rhesus) Normal Tissue: Small Intestine

C1534226 40 reactions
EUR 578

cDNA - Monkey (Cynomolgus) Normal Tissue: Small Intestine

C1534226-Cy 40 reactions
EUR 578

cDNA - Human Adult Normal Tissue: Small Intestine: Ileum

C1234227 40 reactions
EUR 385

cDNA - Monkey (Rhesus) Normal Tissue: Small Intestine: Ileum

C1534227 40 reactions
EUR 578

cDNA - Human Adult Normal Tissue: Small Intestine: Jejunum

C1234230 40 reactions
EUR 385

cDNA - Human Adult Normal Tissue: Small Intestine: Duodenum

C1234101-10 10 reactions
EUR 215

cDNA - Monkey (Rhesus) Normal Tissue: Small Intestine: Jejunum

C1534230 40 reactions
EUR 578

cDNA - Monkey (Rhesus) Normal Tissue: Small Intestine: Duodenum

C1534101 40 reactions
EUR 578

cDNA - Human Diabetic Diseased Tissue: Small Intestine: Ileum

C1236227Dia 40 reactions
EUR 729

cDNA - Monkey (Cynomolgus) Normal Tissue: Small Intestine: Ileum

C1534227-Cy 40 reactions
EUR 578

cDNA - Human Diabetic Diseased Tissue: Small Intestine: Jejunum

C1236230Dia 40 reactions
EUR 729

cDNA - Monkey (Cynomolgus) Normal Tissue: Small Intestine: Jejunum

C1534230-Cy 40 reactions
EUR 578

cDNA - Human Diabetic Diseased Tissue: Small Intestine: Duodenum

C1236101Dia 40 reactions
EUR 729

cDNA - Monkey (Cynomolgus) Normal Tissue: Small Intestine: Duodenum

C1534101-Cy 40 reactions
EUR 578

Small Intestine Lysate

1468 0.1 mg
EUR 229.2
Description: Small Intestine tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Small Intestine Lysate

1308 0.1 mg
EUR 229.2
Description: Small intestine tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Small intestine cancer

SM208 each
EUR 546
Description: Small intestine cancer, metastatic lymph nodes and normal tissue high density tissue microarray, 69 cases/208 core, with stage and grade data

Small intestine disease spectrum (small intestine cancer progression) tissue array

SM802 each
EUR 306
Description: Small intestine disease spectrum (small intestine cancer progression) tissue array, with grade, stage and TNM data, 72 cases/80 cores

Small Intestine Tumor Lysate

1338 0.1 mg
EUR 304.8
Description: Small Intestine tumor tissue lysate was prepared by homogenization in lysis buffer (10 mM HEPES pH7.9, 1.5 mM MgCl2, 10 mM KCl, 1 mM ethylenediaminetetraacetic acid, 10% glycerol, 1% NP-40, and a cocktail of protease inhibitors). Tissue and cell debris was removed by centrifugation. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Small Intestine Membrane Lysate

XBL-10876 0.1 mg
EUR 619.8
Description: Human small intestine tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human small intestine tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated small intestine tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated small intestine tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.

Small Intestine Cytoplasmic Lysate

XBL-10875 0.1 mg
EUR 273.3
Description: Human small intestine tissue cytoplasmic protein lysate was prepared by isolating the cytoplasmic protein from whole tissue homogenates using a proprietary technique. The human small intestine tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The cytoplasmic protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, glycerol, and a cocktail of protease inhibitors. For quality control purposes, the isolated small intestine tissue cytoplasmic protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated small intestine tissue cytoplasmic protein is then Western analyzed by GAPDH antibody, and the expression level is consistent with each lot.

Total RNA - Lupus: Small Intestine

R1236226Lup-50 50 ug
EUR 421.2

Human Small Intestine Tumor lysate

HTL-1338 1 mg
EUR 927.6

Small intestine Tissue Slide (Normal)

11-801-10um 10 um
EUR 241.8

Small intestine Tissue Slide (Normal)

11-801-4um 4 um
EUR 216.6

Normal small intestine tissue array

SMN601 each
EUR 222
Description: Normal small intestine tissue array, including TNM, clinical stage and pathology grade, 30 cases/ 60 cores

Small Intestine Tissue Slide (Abnormal)

11-824-10um 10 um
EUR 241.8

Small Intestine Tissue Slide (Abnormal)

11-824-4um 4 um
EUR 216.6

Small Intestine Membrane Tumor Lysate

XBL-10888 0.1 mg
EUR 752.1
Description: Human small intestine tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human small intestine tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated small intestine tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated small intestine tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.

Small Intestine Tissue Slide (Malignant)

11-814-10um 10 um
EUR 241.8

Small Intestine Tissue Slide (Malignant)

11-814-4um 4 um
EUR 216.6

Small Intestine Liver Cirrhosis Lysate

XBL-10377 0.1 mg
EUR 796.2
Description: Human small intestine tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The human small intestine tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the small intestine tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The small intestine tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.

Human Small Intestine Endothelial Cells

HEC15 500,000+ Cells Frozen
EUR 1296

Rat Small Intestine Whole tissue lysate

RAL-1468 1 mg
EUR 628.8

Small Intestine Tissue Slide (Adenocarcinoma)

11-815-10um 10 um
EUR 241.8

Small Intestine Tissue Slide (Adenocarcinoma)

11-815-4um 4 um
EUR 216.6

Mouse Small Intestine Whole tissue lysate

MAL-1408 1 mg
EUR 628.8

Total RNA - Liver Cirrhosis: Small Intestine

R1236226Lcs-50 50 ug
EUR 421.2

Small intestine carcinoma test tissue array

T421b each
EUR 48
Description: Small intestine carcinoma test tissue array, with normal small intestine tissues, including pathology grade, TNM and clinical stage, 6 cases/24 cores, replacing T421a

Total Protein - Liver Cirrhosis: Small Intestine

P1236226Lcs 1 mg
EUR 471

Total RNA - Rat Normal Tissue: Small Intestine

R1434226-50 50 ug
EUR 209

Total RNA - Human Tumor Tissue: Small Intestine

R1235226-10 10 ug
EUR 400

Genomic DNA - Rat Normal Tissue: Small Intestine

D1434226 100 ug
EUR 286

Total RNA - Mouse Normal Tissue: Small Intestine

R1334226-50 50 ug
EUR 209

Genomic DNA - Mouse Normal Tissue: Small Intestine

D1334226 100 ug
EUR 286

Total Protein - Human Tumor Tissue: Small Intestine

P1235226 1 mg
EUR 471

Matched Pair Tissue Slides - Small Intestine Tissue

11-800-MPPT 1 pack
EUR 241.8
Description: The Matched Pair Paraffin Tissue (MPPT) slides are designed for identifying tumor-specific/metastasis genes or proteins. Slices from normal and malignant tissues are mounted on each MPPT slide which can then be treated as a single histological slide for H&E staining, immunohistochemistry, or in situ hybridization. This format allows a rapid analysis of protein expression and localization across normal and cancerous tissue.

Total Protein - Mouse Normal Tissue: Small Intestine

P1334226 1 mg
EUR 188

Small intestine carcinoma and normal tissue array

SM801 each
EUR 306
Description: Small intestine carcinoma and normal tissue array, 77 cases/80 cores

Membrane Protein - Human Tumor Tissue: Small Intestine

P3235226 0.1 mg
EUR 410

Total Protein - Liver Cirrhosis: Small Intestine: Ileum

P1236227Lcs 1 mg
EUR 471

Multiple Species Tissue Slides - Small Intestine Tissue

11-800-MSTA 1 pack
EUR 241.8
Description: The Multiple Species Tissue Array (MSTA) slides were designed to study protein expression patterns in different cells and tissues from multiple species. Tissue slices from three different species are mounted on each MSTA slide which can then be treated as a single histological slide for H&E staining, immunohistochemistry, or in situ hybridization. This format allows a rapid analysis of protein expression and localization across different species. MSTA slides can also be used to quickly determine the species reactivity of a given antibody.

Total Protein - Liver Cirrhosis: Small Intestine: Jejunum

P1236230Lcs 1 mg
EUR 471

Total RNA - Human Adult Normal Tissue: Small Intestine

R1234226-50 50 ug
EUR 170

Total Protein - Liver Cirrhosis: Small Intestine: Duodenum

P1236101Lcs 1 mg
EUR 471

Total RNA - Monkey (Rhesus) Normal Tissue: Small Intestine

R1534226-50 50 ug
EUR 191

Probable Small Intestine Urate Exporter (SLC17A4) Antibody

20-abx312468
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Monkey (Cyno.) Normal Small Intestine Whole tissue lysate

MCL-1205 1 mg
EUR 628.8

Human Adult Small Intestine (Normal) Whole tissue lysate

HAL-1308 1 mg
EUR 628.8

Total Protein - Human Adult Normal Tissue: Small Intestine

P1234226 1 mg
EUR 188

Monkey (Rhesus) Normal Small Intestine Whole tissue lysate

MRL-1316 1 mg
EUR 628.8

Frozen Tissue Section - Human Adult Normal: Small Intestine

T1234226 5 slides
EUR 343

Genomic DNA - Lupus: Small Intestine, from a single donor

D1236226Lup 50 ug
EUR 504

Total Protein - Human Tumor Tissue: Small Intestine: Duodenum

P1235101 1 mg
EUR 471

Total RNA - Monkey (Cynomolgus) Normal Tissue: Small Intestine

R1534226-Cy 50 ug
EUR 191

Glucose Transporter Type 5, Small Intestine (GLUT5) Antibody

20-abx133767
  • EUR 360.00
  • EUR 526.80
  • EUR 226.80
  • 100 ul
  • 200 ul
  • 30 ul

Glucose Transporter Type 5, Small Intestine (GLUT5) Antibody

abx430535-200ul 200 ul
EUR 460.8

Membrane Protein - Human Adult Normal Tissue: Small Intestine

P3234226 0.1 mg
EUR 270

Paraffin Tissue Section - Human Adult Normal: Small Intestine

T2234226 5 slides
EUR 185

Total RNA - Human Adult Normal Tissue: Small Intestine: Ileum

R1234227-50 50 ug
EUR 170

Human, Mouse, Rat - Small Intestine Tissue Array (5 slides/pk)

TAS-1020 1 pk
EUR 343.2

Total RNA - Human Adult Normal Tissue: Small Intestine: Jejunum

R1234230-50 50 ug
EUR 170

Total RNA - Monkey (Rhesus) Normal Tissue: Small Intestine: Ileum

R1534227-50 50 ug
EUR 191

Total RNA - Human Adult Normal Tissue: Small Intestine: Duodenum

R1234101-50 50 ug
EUR 170

Small intestine disease (cancer progression) spectrum tissue array

SM2081 each
EUR 546
Description: Small intestine disease (cancer progression) spectrum tissue array, 95 cases/208 cores

Total Protein - Human Adult Normal Tissue: Small Intestine: Ileum

P1234227 1 mg
EUR 188

Total RNA - Monkey (Rhesus) Normal Tissue: Small Intestine: Duodenum

R1534101-50 50 ug
EUR 353

Total Protein - Human Adult Normal Tissue: Small Intestine: Jejunum

P1234230 1 mg
EUR 188

Membrane Protein - Human Adult Normal Tissue: Small Intestine: Ileum

P3234227 0.1 mg
EUR 270

Total Protein - Human Adult Normal Tissue: Small Intestine: Duodenum

P1234101 1 mg
EUR 188

Genomic DNA - Liver Cirrhosis: Small Intestine, from a single donor

D1236226Lcs 50 ug
EUR 489

Membrane Protein - Human Adult Normal Tissue: Small Intestine: Duodenum

P3234101 0.1 mg
EUR 270

Membrane Protein - Human Adult Normal Tissue: Small Intestine: Jejunum

P3234230 0.1 mg
EUR 270

Total Protein - Human Diabetic Diseased Tissue: Small Intestine: Ileum

P1236227Dia 1 mg
EUR 471

Total RNA - Monkey (Cynomolgus) Normal Tissue: Small Intestine: Duodenum

R1534101-Cy 50 ug
EUR 353

Total Protein - Human Diabetic Diseased Tissue: Small Intestine: Duodenum

P1236101Dia 1 mg
EUR 471

Total Protein - Human Diabetic Diseased Tissue: Small Intestine: Jejunum

P1236230Dia 1 mg
EUR 471

Total RNA - Human Adult Normal Tissue 5 Donor Pool: Small Intestine

R1234226-P 50 ug
EUR 370

Rat Normal Small intestine (single section per slide) (5 slides/pack)

RaFPT036 5 slides
EUR 150

Mouse Normal Small intestine (single section per slide) (5 slides/pack)

MoFPT041 5 slides
EUR 150

Human Small intestine Cancer (single section per slide) (5 slides/pack)

HuCAT091 5 slides
EUR 150

copper nanowires as nanofertilizers to plant alfalfa: Understanding the interaction of nano-bio systems of microbial genomics, plant molecular response and spectroscopic studies

The latest applications of nano copper (Cu) in agrosystem compounds have demonstrated the potential to improve the performance of plant physiological and agronomic parameters. We grew alfalfa (Medicago sativa) in potting mix changed with bulk, nano, and Cu ionic compound at 80 and 280 mg Cu / kg; then, we evaluate the performance of the plant at the physiological and molecular level.

Plants treated with the precipitation / Cu nano served better agronomic response. P and S content is reduced in bulk and ion Cu-exposed plants, compared with controls (p ≤ 0.05). All forms of Cu increase the content of Fe and Zn in the Fe in the roots and leaves, compared to controls (p ≤ 0.05).

Leaf-superoxide dismutase expression was plus ~ 27-fold and Rubisco mRNA was not affected in bulk / nano Cu-treated plants, compared with controls (p ≤ 0.05). Bulk / nano Cu increased relative abundance of microorganisms involved in uptake. These results indicate that the nano Cu improve the physiology of alfalfa and could be considered as a potential nanofertilizers.

cDNA - Monkey (Cynomolgus) Normal Tissue: Kidney

C1534142-Cy 40 reactions
EUR 385

Matched Pair - cDNA - Human Primary Tumor and Normal Tissue: Kidney

C8235142-PP 10 reactions x2
EUR 530

Matched Pair - cDNA - Human Primary and Matched Metastatic Tumor Tissue: Kidney

C8235142-PM 10 reactions x2
EUR 1102

Multiple kidney tumor with kidney tissue array

KD1501a each
EUR 258
Description: Multiple kidney tumor with kidney tissue array, including pathology grade, TNM and clinical stage (reference AJCC 7th version), 75 cases/150 cores, replacing KD1501

Kidney cancer with matched kidney tissue array

KD801a each
EUR 258
Description: Kidney cancer with matched kidney tissue array, including pathology grade, TNM and clinical stagr, 40 cases/80 cores, replacing KD801

Kidney carcinoma with matched kidney tissue array

KD244a each
EUR 48
Description: Kidney carcinoma with matched kidney tissue array, including pathology grade, TNM and clinical stage, 12 cases/24 cores, replacing KD244

Kidney Lysate

1465 0.1 mg
EUR 229.2
Description: Kidney tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Kidney Lysate

21-104 0.1 mg
EUR 342.6
Description: Bovine kidney tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The bovine kidney tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the kidney tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The kidney tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.

Kidney Lysate

21-116 0.1 mg
EUR 342.6
Description: Guinea Pig kidney tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The guinea pig kidney tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the kidney tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The kidney tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.

Kidney Lysate

21-190 0.1 mg
EUR 342.6
Description: Monkey (Cynomolgus) kidney tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The monkey (Cynomolgus) kidney tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the kidney tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The kidney tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.

Kidney Lysate

21-405 0.1 mg
EUR 342.6
Description: Porcine kidney tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The porcine kidney tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the kidney tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The kidney tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.

Kidney Lysate

21-418 0.1 mg
EUR 342.6
Description: Rabbit kidney tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The rabbit kidney tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the kidney tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The kidney tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.

Rat Kidney PrimaCell2: Normal Kidney Epithelial Cells

2-82556 1 Kit Ask for price

Mouse Kidney PrimaCell2: Normal Kidney Epithelial Cells

2-82057 1 Kit Ask for price

Human Kidney PrimaCell2: Normal Kidney Epithelial Cells

2-96075 1 Kit Ask for price

Kidney cancer with kidney tissue test tissue array

T071b each
EUR 48
Description: Kidney cancer with kidney tissue test tissue array, including pathology grade, TNM and clinical stage, 6 cases/24 cores, replacing T071a

Kidney cancer tissue array with normal Kidney tissue

KD2082a each
EUR 474
Description: Kidney cancer tissue array with normal Kidney tissue, including TNM and pathology grade, 96 cases/192 cores, replacing KD2082

Normal kidney and normal adjacent kidney tissue array

KDN241a each
EUR 138
Description: Normal kidney and normal adjacent kidney tissue array, 24 cases/ 24 cores

Multiple kidney cancer with matched kidney tissue array

KD461 each
EUR 258
Description: Multiple kidney cancer with matched kidney tissue array, including pathology grade, TNM and clinical stage, 23 cases/46 cores

Kidney disease spectrum (kidney cancer progression) tissue array

BC07001 each
EUR 258
Description: Kidney disease spectrum (kidney cancer progression) tissue array, including TNM and pathology grade, 40 cases/80 cores (core size 1.5mm)

Human Kidney CAFs

CAF110 1,000,000 cells Ask for price

Kidney cancer tissue array with adjacent normal kidney tissue

BC07015b each
EUR 168
Description: Kidney cancer tissue array with adjacent normal kidney tissue, including TNM, clinical stage and pathology grade, 72 cases/72 cores, replacing BC07015a

Kidney cancer with matched adjacent normal kidney tissue array

KD603 each
EUR 204
Description: Kidney cancer with matched adjacent normal kidney tissue array, containing metastatic clear cell carcinoma, including pathology grade, TNM and clinical stage (reference AJCC 8th version), 33 cases/61 cores

Kidney cancer (CCRCC

KD1201 each
EUR 306
Description: Kidney cancer (CCRCC,PRCC,CRCC)with kidney tissue array,including ISUP Grade, TNM/Stage, 120 cases/120 cores (core size 1.0mm ), (Previous Item# is BC07115b)

Kidney tissue array

KD803b each
EUR 354
Description: Kidney tissue array, 80 cases/80n cores (core size 1.5mm), replacing KD803a

Kidney Lupus Lysate

XBL-10357 0.1 mg
EUR 796.2
Description: Human kidney tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The human kidney tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the kidney tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The kidney tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.

Fetal Kidney Lysate

XBL-10408 0.1 mg
EUR 342.6
Description: Fetal human kidney tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The fetal human kidney tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the kidney tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The kidney tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.

Rat Kidney PrimaCell2: Normal Kidney Epithelial Cells Growth Medium

9-25056 5 x 100 ml Ask for price

Mouse Kidney PrimaCell2: Normal Kidney Epithelial Cells Growth Medium

9-32057 5 x 100 ml Ask for price

Human Kidney PrimaCell2: Normal Kidney Epithelial Cells Growth Medium

9-46075 5 x 100 ml Ask for price

Kidney Cancer Exosome

P141-KN - Ask for price

Kidney Membrane Lysate

XBL-10649 0.1 mg
EUR 619.8
Description: Human kidney tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human kidney tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated kidney tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated kidney tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.

Kidney tumor tissue array with matched tumor adjacent kidney tissue

KD2602a each
EUR 354
Description: Kidney tumor tissue array with matched tumor adjacent kidney tissue, including TNM, clinical stage and pathology grade, 65 cases/260 cores, replacing KD2602

Rat Kidney Tissue Preparation Buffer 2: Normal Kidney Epithelial Cells

9-80263 1 x 100 ml Ask for price

Kidney cancer tissue array with matched cancer adjacent kidney tissue

KD2601a each
EUR 354
Description: Kidney cancer tissue array with matched cancer adjacent kidney tissue,including TNM, clinical stage and pathology grade, 65 cases/ 260 cores, replacing KD2061

Kidney clear cell carcinoma with matched adjacent kidney tissue array

KD486 each
EUR 168
Description: Kidney clear cell carcinoma with matched adjacent kidney tissue array, including TNM and pathology grade, 16 cases/48 cores

Kidney cancer tissue array with matched adjacent normal kidney tissue

KD901a each
EUR 258
Description: Kidney cancer tissue array with matched adjacent normal kidney tissue, including TNM, clinical stage and pathology grade, 40 cases/90 cores, replacing KD901

Human Kidney Tissue Preparation Buffer 2: Normal Kidney Epithelial Cells

9-80075 1 x 100 ml Ask for price

Mouse Kidney Tissue Preparation Buffer 2: Normal Kidney Epithelial Cells

9-80171 1 x 100 ml Ask for price

Total RNA - Lupus: Kidney

R1236142Lup-50 50 ug
EUR 421.2

Multiple kidney cancer with matched tumor adjacent (AT) kidney tissue array

KD702a each
EUR 234
Description: Multiple kidney cancer with matched tumor adjacent (AT) kidney tissue array, 35 cases/70 cores, including CCRCC (15 cases), PRCC (9 cases), CHRCC (8 cases), ccpRCC (3 cases)

Multiple kidney disease with kidney tissue array (2016/ISUP WHO classification

KD804a each
EUR 258
Description: Multiple kidney disease with kidney tissue array (2016/ISUP WHO classification, Grade mean Isup Grade), including pathoogy grade, TNM and clinical stage (reference AJCC 8th version), 75 cases/80 cores, replacing KD804

Rat Kidney Tissue Lysate

LYSATE0006 200ug
EUR 180
Description: This cell lysate is prepared from rat kidney tissue using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

Plastic Kidney Bowl 252mm

INS6272 EACH
EUR 7.2

CK KIDNEY WHOLE 25 EA*

43020-2 25 EA
EUR 288.11

Kidney Tissue Slide (Tumor)

10-402-10um 10 um
EUR 241.8

Kidney Tissue Slide (Tumor)

10-402-4um 4 um
EUR 216.6

Kidney Cancer Exosome RNA

P241-KN - Ask for price

Human Kidney Tumor lysate

HTL-1324 1 mg
EUR 927.6

Pig WHOLE KIDNEY 10 EA*

59420-2 10 EA
EUR 285.49

Kidney Tissue Slide (Normal)

10-401-10um 10 um
EUR 241.8

Kidney Tissue Slide (Normal)

10-401-4um 4 um
EUR 216.6

Kidney Tissue Lysate (Tumor)

1707-01 0.1 mg
EUR 336.3
Description: Kidney tumor tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Kidney Tissue Lysate (Tumor)

1707-02 0.1 mg
EUR 336.3
Description: Kidney tumor tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Kidney Tissue Lysate (Tumor)

1707-03 0.1 mg
EUR 336.3
Description: Kidney tumor tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Kidney Tissue Lysate (Tumor)

1707-04 0.1 mg
EUR 336.3
Description: Kidney tumor tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Kidney Tissue Lysate (Tumor)

1707-05 0.1 mg
EUR 336.3
Description: Kidney tumor tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Kidney Tissue Lysate (Tumor)

1707-06 0.1 mg
EUR 336.3
Description: Kidney tumor tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Kidney Tissue Lysate (Tumor)

1707-07 0.1 mg
EUR 336.3
Description: Kidney tumor tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Kidney Tissue Lysate (Tumor)

1707-08 0.1 mg
EUR 336.3
Description: Kidney tumor tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Kidney Tissue Lysate (Tumor)

1707-09 0.1 mg
EUR 336.3
Description: Kidney tumor tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Kidney Tissue Lysate (Tumor)

1708-01 0.1 mg
EUR 336.3
Description: Kidney tumor tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Kidney Tissue Lysate (Tumor)

1708-02 0.1 mg
EUR 336.3
Description: Kidney tumor tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Kidney Tissue Lysate (Tumor)

1708-03 0.1 mg
EUR 336.3
Description: Kidney tumor tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Kidney Tissue Lysate (Tumor)

1709-01 0.1 mg
EUR 336.3
Description: Kidney tumor tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Kidney Tissue Lysate (Tumor)

1709-02 0.1 mg
EUR 336.3
Description: Kidney tumor tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Kidney Tissue Lysate (Tumor)

1709-03 0.1 mg
EUR 336.3
Description: Kidney tumor tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Human Kidney Tissue Lysate

30R-AK003 150 ug
EUR 262.8
Description: Fresh tissue lysate isolated from human kidney

Kidney cancer tissue array

BC07014b each
EUR 168
Description: Kidney cancer tissue array, including pathology grade, TNM and clinical stage, 72 cases/72 cores, replacing BC07014a

Kidney cancer tissue array

KD245 each
EUR 66
Description: Kidney cancer tissue array, including TNM, clinical stage and pathology grade, 24 cases/ 24 cores

Kidney cancer tissue array

KD485 each
EUR 168
Description: Kidney cancer tissue array, with normal tissue, including TNM and pathology grade, 48 cases/48 cores

Normal kidney tissue array

KD803 each
EUR 354
Description: Normal kidney tissue array, replacing BN07012, 80 cases/80 cores

Normal kidney tissue array

KDN242 each
EUR 72
Description: Normal kidney tissue array, 24 cases/24 cores

Mouse Kidney Tissue Lysate

LYSATE0016 200ug
EUR 180
Description: This cell lysate is prepared from mouse kidney tissue using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

Total Protein - Lupus: Kidney

P1236142Lup 1 mg
EUR 486

Kidney cancer (grade I) tissue array with matched adjacent normal kidney tissue

KD701a each
EUR 234
Description: Kidney cancer (grade I) tissue array with matched adjacent normal kidney tissue, including TNM, clinical stage and pathology grade, 35 cases/70 cores, replacing KD701

Kidney Tissue Lysate (Normal)

1706-02 0.1 mg
EUR 260.7
Description: Kidney tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Kidney Tissue Lysate (Normal)

1706-03 0.1 mg
EUR 260.7
Description: Kidney tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Kidney Tissue Lysate (Normal)

1706-04 0.1 mg
EUR 260.7
Description: Kidney tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Kidney Tissue Lysate (Normal)

1706-05 0.1 mg
EUR 260.7
Description: Kidney tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Kidney Tissue Slide (Abnormal)

10-455-10um 10 um
EUR 241.8

Kidney Tissue Slide (Abnormal)

10-455-4um 4 um
EUR 216.6

Mouse Kidney Nuclear Extract

X12011 500 µg Ask for price

Kidney Membrane Tumor Lysate

XBL-10661 0.1 mg
EUR 752.1
Description: Human kidney tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human kidney tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated kidney tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated kidney tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.

Kidney Lysate (0 Days Old)

1405-0 0.1 mg
EUR 229.2
Description: Kidney tissue lysate (0 Days Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Kidney Lysate (14 Days Old)

1405-14 0.1 mg
EUR 229.2
Description: Kidney tissue lysate (14 Days Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Leave a Reply

Your email address will not be published.