Comparative genomics and pangenome-oriented studies reveal high homogeneity

Comparative genomics and pangenome-oriented studies reveal high homogeneity of the agronomically relevant enterobacterial plant pathogen Dickeya solani

 

Comparative genomics and pangenome-oriented studies reveal high homogeneity

Background: Dickeya solani is an important plant pathogenic bacteria that caused heavy losses in the production of potatoes in Europe. This species attracted much attention because of the virulence of extraordinary potential to destroy the large and spread more easily contrasted with other spp Dickeya. Given the high need for extensive studies on important economic soft rot Pectobacteriaceae, we perform comparative genomics analysis on the strain D. solani to search for the genetic foundation that would explain the difference in the level of virulence observed in D. solani population.


Results: High quality assemblies from 8 de novo genome sequencing D. solani has been obtained. Whole-sequence comparison, Anib, anim, Tetra and oriented pangenome analysis performed at the genome and sequences of 14 additional strains revealed a very high degree of homogeneity among the studied genetic material D. solani strains. By using 22 genomes, pangenome D. solani, which consists of a core of 84.7%, 7.2% and genes unique accessory 8.1%, has been almost completely determined, indicates the structure pangenome almost closed.

Attribution of genes included in D. solani pangenome fraction of COG functional categories shows that a higher percentage of unique accessories and pangenome different parts of the core part encountered in phage / mobile elements and transcription- groups associated with RNS genome 05.1.2A yeast strains have the most significant impact. Also, D. solani first large-scale genome-wide gene phylogeny calculated alignments core of this serial here reported.


Conclusion: The status almost closed D. solani pangenome achieved in this work points to the fact that the unique genes from these species should no longer growing. these features are characteristic of a good representative taxa occupy isolated ecological niches or lack of efficient mechanisms to exchange genes and recombination, which seems rational about strict pathogen species with clonal population structure. Finally, there is no clear correlation between the geographic origin of strains of D. solani and phylogeny they were found, which may reflect the peculiarities of the international potato seed market.

 Comparative genomics and pangenome-oriented studies reveal high homogeneity of the agronomically relevant enterobacterial plant pathogen Dickeya solani
Comparative genomics and pangenome-oriented studies reveal high homogeneity of the agronomically relevant enterobacterial plant pathogen Dickeya solani

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Comparative Genomics Identifies Genetic Locus in Plant-Associated Pseudomonas spp. It Is Necessary to Induced Systemic Vulnerabilities

Plant root-associated microbes promote plant growth and bring induced systemic resistance (ISR) to leaf pathogens. In an effort to find growth-promoting novel and ISR-inducing strains, we previously identified the root-associated strains of Pseudomonas spp. which promote plant growth but suddenly raises induction of systemic vulnerability (ISS) than the ISR to leaf pathogens.

Here, we show that the ISS-inducing phenotype is common among the root-associated Pseudomonas spp. Using comparative genomics, we identified a single locus of Pseudomonas fluorescens strain that is unique to ISS. We generated a net removal of the 11-gene loci ISS and found it necessary to phenotype ISS. Although the function of genes estimated in the locus that is not clear based on the similarity with genes of known function, the locus of the ISS is present in a variety of bacteria, and the part of the gene that was previously involved in the pathogenesis in animals. Collectively, these data suggest that a single locus contributes systemic bacterial plant modulation Microbiome immunity.

IMPORTANCE related bacteria can have various effects on the health of their hosts, but the genetic and molecular basis of these effects have largely remained elusive. This work indicates that the locus of new bacteria can regulate systemic plant immunity.

In addition, this work suggests that the growth-promoting strain may have unexpected consequences for the plant immunity, and this is very important to consider when microbiome plant is being engineered for agronomic improvements.

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Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

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T1236045Msc 5 slides
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Frozen Tissue Section - Multiple Sclerosis Disease: Brain: Frontal Lobe

T1236051Msc 5 slides
EUR 465.00
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Frozen Tissue Section - Multiple Sclerosis Disease: Brain: Medulla oblongata

T1236057Msc 5 slides
EUR 465.00
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Frozen Tissue Section - Multiple Sclerosis Disease: Brain: Occipital Lobe

T1236062Msc 5 slides
EUR 465.00
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Frozen Tissue Section - Multiple Sclerosis Disease: Brain: Parietal Lobe

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EUR 465.00
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T1236072Msc 5 slides
EUR 465.00
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Frozen Tissue Section - Multiple Sclerosis Disease: Brain: Precentral Gyrus

T1236073Msc 5 slides
EUR 465.00
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Frozen Tissue Section - Multiple Sclerosis Disease: Brain: Temporal Lobe

T1236078Msc 5 slides
EUR 465.00
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Paraffin Tissue Section - Multiple Sclerosis Disease: Brain: Corpus Callosum

T2236045Msc 5 slides
EUR 257.00
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Paraffin Tissue Section - Multiple Sclerosis Disease: Brain: Frontal Lobe

T2236051Msc 5 slides
EUR 257.00
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Paraffin Tissue Section - Multiple Sclerosis Disease: Brain: Medulla oblongata

T2236057Msc 5 slides
EUR 257.00
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Paraffin Tissue Section - Multiple Sclerosis Disease: Brain: Occipital Lobe

T2236062Msc 5 slides
EUR 257.00
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Paraffin Tissue Section - Multiple Sclerosis Disease: Brain: Parietal Lobe

T2236066Msc 5 slides
EUR 257.00
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Paraffin Tissue Section - Multiple Sclerosis Disease: Brain: Postcentral Gyrus

T2236072Msc 5 slides
EUR 257.00
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Paraffin Tissue Section - Multiple Sclerosis Disease: Brain: Precentral Gyrus

T2236073Msc 5 slides
EUR 257.00
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Paraffin Tissue Section - Multiple Sclerosis Disease: Brain: Temporal Lobe

T2236078Msc 5 slides
EUR 257.00
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Genomic DNA from Multiple Sclerosis Disease: Brain, from a single donor

D1236035Msc 50 ug
EUR 446.00
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Multiple Sclerosis Disease: Brain: Thalamus, from a single donor

D1236079Msc 50 ug
EUR 446.00
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Multiple Sclerosis Disease: Brain: Amygdala, from a single donor

D1236036Msc 50 ug
EUR 446.00
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Multiple Sclerosis Disease: Brain: Cerebellum, from a single donor

D1236039Msc 50 ug
EUR 446.00
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Multiple Sclerosis Disease: Brain: Pons, from a single donor

D1236071Msc 50 ug
EUR 446.00
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Multiple Sclerosis Disease: Brain: Temporal Lobe, from a single donor

D1236078Msc 50 ug
EUR 446.00
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Multiple Sclerosis Disease: Brain: Frontal Lobe, from a single donor

D1236051Msc 50 ug
EUR 446.00
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Multiple Sclerosis Disease: Brain: Medulla oblongata, from a single donor

D1236057MSC 50 ug
EUR 446.00
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Multiple Sclerosis Disease: Brain: Occipital Lobe, from a single donor

D1236062Msc 50 ug
EUR 446.00
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Multiple Sclerosis Disease: Brain: Parietal Lobe, from a single donor

D1236066Msc 50 ug
EUR 446.00
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Multiple Sclerosis Disease: Brain: Postcentral Gyrus, from a single donor

D1236072Msc 50 ug
EUR 446.00
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Genomic DNA from Multiple Sclerosis Disease: Brain: Precentral Gyrus, from a single donor

D1236073Msc 50 ug
EUR 446.00
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Alzheimer's Disease: Brain

C1236035Alz 40 reactions
EUR 668.00
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Parkinson's Disease: Brain

C1236035Par 40 reactions
EUR 668.00
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Alzheimer's Disease: Brain: Amygdala

C1236036Alz 40 reactions
EUR 668.00
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Alzheimer's Disease: Brain: Cerebellum

C1236039Alz 40 reactions
EUR 668.00
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Alzheimer's Disease: Brain: Hippocampus

C1236052Alz 40 reactions
EUR 801.00
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Parkinson's Disease: Brain: Hippocampus

C1236052Par 40 reactions
EUR 801.00
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Alzheimer's Disease: Brain: Pons

C1236071Alz 40 reactions
EUR 668.00
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Alzheimer's Disease: Brain: Thalamus

C1236079Alz 40 reactions
EUR 668.00
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Congenital heart disease: Heart

C1236122Hd-3 40 reactions
EUR 668.00
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Alzheimer's Disease: Brain: Frontal Lobe

C1236051Alz 40 reactions
EUR 668.00
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Alzheimer's Disease: Brain: Occipital Lobe

C1236062Alz 40 reactions
EUR 668.00
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Alzheimer's Disease: Brain: Parietal Lobe

C1236066Alz 40 reactions
EUR 668.00
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Alzheimer's Disease: Brain: Postcentral Gyrus

C1236072Alz 40 reactions
EUR 668.00
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Alzheimer's Disease: Brain: Precentral Gyrus

C1236073Alz 40 reactions
EUR 668.00
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Alzheimer's Disease: Brain: Temporal Lobe

C1236078Alz 40 reactions
EUR 668.00
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Tumor Tissue: Lymphoma, Hodgkin's Disease

C1235161A-10 10 reactions
EUR 282.00
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Tumor Tissue: Lymphoma, Non-Hodgkin's Disease

C1235161B-10 10 reactions
EUR 282.00
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA Synthesis SuperMix

20-abx09801420ulSystems
  • EUR 565.00
  • EUR 481.00
  • 100 rxns × 20 ul Systems
  • 50 rxns × 20 ul Systems
  • Shipped within 5-10 working days.

Evo? cDNA Kit

M1164-100
EUR 294.00

Evo? cDNA Kit

M1164-25
EUR 234.00

Novo? cDNA Kit

M1165-100
EUR 354.00

Novo? cDNA Kit

M1165-25
EUR 267.00

Evo? cDNA Supermix

M1168-100
EUR 381.00

Evo? cDNA Supermix

M1168-25
EUR 267.00

Novo? cDNA Supermix

M1169-100
EUR 441.00

Novo? cDNA Supermix

M1169-25
EUR 289.00

Multiple Line Reader

LFRB-001 Each
EUR 3916.66
  • Product Category: Lateral-Flow Readers and Equipment
Description: Multiple Line Reader is a portable universal lateral-flow reader that is based on colour change method.

Tuberous Sclerosis 1 Antibody

20-abx116334
  • EUR 732.00
  • EUR 398.00
  • 150 ul
  • 50 ul
  • Shipped within 5-10 working days.

cDNA from Plant Normal Tissue: cDNA from Plant: Arabidopsis

C1634310 40 reactions
EUR 621.00
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

 

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