Comparative genomics and pangenome-oriented studies reveal high homogeneity

Comparative genomics and pangenome-oriented studies reveal high homogeneity of the agronomically relevant enterobacterial plant pathogen Dickeya solani

 

Comparative genomics and pangenome-oriented studies reveal high homogeneity

Background: Dickeya solani is an important plant pathogenic bacteria that caused heavy losses in the production of potatoes in Europe. This species attracted much attention because of the virulence of extraordinary potential to destroy the large and spread more easily contrasted with other spp Dickeya. Given the high need for extensive studies on important economic soft rot Pectobacteriaceae, we perform comparative genomics analysis on the strain D. solani to search for the genetic foundation that would explain the difference in the level of virulence observed in D. solani population.


Results: High quality assemblies from 8 de novo genome sequencing D. solani has been obtained. Whole-sequence comparison, Anib, anim, Tetra and oriented pangenome analysis performed at the genome and sequences of 14 additional strains revealed a very high degree of homogeneity among the studied genetic material D. solani strains. By using 22 genomes, pangenome D. solani, which consists of a core of 84.7%, 7.2% and genes unique accessory 8.1%, has been almost completely determined, indicates the structure pangenome almost closed.

Attribution of genes included in D. solani pangenome fraction of COG functional categories shows that a higher percentage of unique accessories and pangenome different parts of the core part encountered in phage / mobile elements and transcription- groups associated with RNS genome 05.1.2A yeast strains have the most significant impact. Also, D. solani first large-scale genome-wide gene phylogeny calculated alignments core of this serial here reported.


Conclusion: The status almost closed D. solani pangenome achieved in this work points to the fact that the unique genes from these species should no longer growing. these features are characteristic of a good representative taxa occupy isolated ecological niches or lack of efficient mechanisms to exchange genes and recombination, which seems rational about strict pathogen species with clonal population structure. Finally, there is no clear correlation between the geographic origin of strains of D. solani and phylogeny they were found, which may reflect the peculiarities of the international potato seed market.

 Comparative genomics and pangenome-oriented studies reveal high homogeneity of the agronomically relevant enterobacterial plant pathogen Dickeya solani
Comparative genomics and pangenome-oriented studies reveal high homogeneity of the agronomically relevant enterobacterial plant pathogen Dickeya solani

Rat Universal Reference cDNA-Oligo dT

RD-UR-80 2x40 reactions
EUR 461

Pig Universal Reference cDNA-Oligo dT

PD-UR-40 1x40 reactions
EUR 243

Pig Universal Reference cDNA-Oligo dT

PD-UR-80 2x40 reactions
EUR 461

Human Universal Reference cDNA-Oligo dT

HD-UR-40 1x40 reactions
EUR 280

Human Universal Reference cDNA-Oligo dT

HD-UR-80 2x40 reactions
EUR 498

Mouse Universal Reference cDNA-Oligo dT

MD-UR-40 1x40 reactions
EUR 243

Mouse Universal Reference cDNA-Oligo dT

MD-UR-80 2x40 reactions
EUR 461

Sheep Universal Reference cDNA-Oligo dT

SD-UR-40 1x40 reactions
EUR 243

Sheep Universal Reference cDNA-Oligo dT

SD-UR-80 2x40 reactions
EUR 461

Monkey Universal Reference cDNA-Oligo dT

KD-UR-40 1x40 reactions
EUR 280

Monkey Universal Reference cDNA-Oligo dT

KD-UR-80 2x40 reactions
EUR 498

Chicken Universal Reference cDNA-Oligo dT

CD-UR-40 1x40 reactions
EUR 243

Chicken Universal Reference cDNA-Oligo dT

CD-UR-80 2x40 reactions
EUR 461

Dog Universal Reference cDNA-Random Hexamer

DD-UR-40-RH 1x40 reactions
EUR 280

Dog Universal Reference cDNA-Random Hexamer

DD-UR-80-RH 2x40 reactions
EUR 498

Rat Universal Reference cDNA-Random Hexamer

RD-UR-40-RH 1x40 reactions
EUR 243

Rat Universal Reference cDNA-Random Hexamer

RD-UR-80-RH 2x40 reactions
EUR 461

Pig Universal Reference cDNA-Random Hexamer

PD-UR-40-RH 1x40 reactions
EUR 243

Pig Universal Reference cDNA-Random Hexamer

PD-UR-80-RH 2x40 reactions
EUR 461

Human Universal Reference cDNA-Random Hexamer

HD-UR-40-RH 1x40 reactions
EUR 280

Human Universal Reference cDNA-Random Hexamer

HD-UR-80-RH 2x40 reactions
EUR 498

Mouse Universal Reference cDNA-Random Hexamer

MD-UR-40-RH 1x40 reactions
EUR 243

Mouse Universal Reference cDNA-Random Hexamer

MD-UR-80-RH 2x40 reactions
EUR 461

Sheep Universal Reference cDNA-Random Hexamer

SD-UR-40-RH 1x40 reactions
EUR 243

Sheep Universal Reference cDNA-Random Hexamer

SD-UR-80-RH 2x40 reactions
EUR 461

Monkey Universal Reference cDNA-Random Hexamer

KD-UR-40-RH 1x40 reactions
EUR 280

Monkey Universal Reference cDNA-Random Hexamer

KD-UR-80-RH 2x40 reactions
EUR 498

Chicken Universal Reference cDNA-Random Hexamer

CD-UR-40-RH 1x40 reactions
EUR 243

Chicken Universal Reference cDNA-Random Hexamer

CD-UR-80-RH 2x40 reactions
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Dog Universal Reference Total RNA

DR-UR-100 1x0.1mg
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Dog Universal Reference Total RNA

DR-UR-200 2x0.1mg
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Dog Universal Reference Total RNA

DR-UR-400 4x0.1mg
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Rat Universal Reference Total RNA

RR-UR-100 1x0.1mg
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Rat Universal Reference Total RNA

RR-UR-200 2x0.1mg
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Rat Universal Reference Total RNA

RR-UR-400 4x0.1mg
EUR 582

Pig Universal Reference Total RNA

PR-UR-100 1x0.1mg
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Pig Universal Reference Total RNA

PR-UR-200 2x0.1mg
EUR 358

Pig Universal Reference Total RNA

PR-UR-400 4x0.1mg
EUR 582

Mouse Universal Reference Total RNA

MR-UR-100 1x0.1mg
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Mouse Universal Reference Total RNA

MR-UR-200 2x0.1mg
EUR 358

Mouse Universal Reference Total RNA

MR-UR-400 4x0.1mg
EUR 582

Sheep Universal Reference Total RNA

SR-UR-100 1x0.1mg
EUR 187

Sheep Universal Reference Totol RNA

SR-UR-200 2x0.1mg
EUR 358

Sheep Universal Reference Total RNA

SR-UR-400 4x0.1mg
EUR 582

Human Universal Reference Total RNA

HR-UR-50 1x0.05mg
EUR 187

Monkey Universal Reference Total RNA

KR-UR-100 1x0.1mg
EUR 187

Monkey Universal Reference Total RNA

KR-UR-200 2x0.1mg
EUR 358

Monkey Universal Reference Total RNA

KR-UR-400 4x0.1mg
EUR 582

Chicken Universal Reference Total RNA

CR-UR-100 1x0.1mg
EUR 187

Chicken Universal Reference Total RNA

CR-UR-200 2x0.1mg
EUR 358

Chicken Universal Reference Total RNA

CR-UR-400 4x0.1mg
EUR 582

200UL "MAXYMUM RECOVERY" UNIVERSAL FIT REFERENCE TIPS

TR-222-C-L 1000/pk
EUR 297.6
Description: Non Filter Tips; Maxymum Recovery Pipette Tips - Axygen

200UL "MAXYMUM RECOVERY" UNIVERSAL FIT REFERENCE TIPS, RACKED

TR-222-C-L-R 960/pk
EUR 187.2
Description: Non Filter Tips; Maxymum Recovery Pipette Tips - Axygen

200UL YELLOW, STERILIZED UNIVERSAL FIT PIPET TIPS WITH REFERENCE LINES

TR-222-Y-R-S 960/pk
EUR 168
Description: Non Filter Tips; Pipette Tips - Axygen

200UL CLEAR MAXYMUM RECOVERY UNIVERSAL FIT PIPET TIPS WITH REFERENCE LINES

TR-222-C-L-STK 480/pk
EUR 162
Description: Non Filter Tips; Maxymum Recovery Pipette Tips - Axygen

200UL "MAXYMUM RECOVERY" UNIVERSAL FIT REFERENCE TIPS, RACKED AND PRE-STERILIZED

TR-222-C-L-R-S 960/pk
EUR 230.4
Description: Non Filter Tips; Maxymum Recovery Pipette Tips - Axygen

200UL CLEAR, MAXYMUM RECOVERY STERILE UNIVERSAL FIT PIPET TIPS WITH REFERENCE LINES

TR-222-C-STK-S 480/pk
EUR 158.4
Description: Non Filter Tips; Pipette Tips - Axygen

200UL CLEAR PRE-STERILIZED MAXYMUM RECOVERY UNIVERSAL FIT PIPET TIPS WITH REFERENCE LINES

TR-222-C-L-STK-S 480/pk
EUR 184.8
Description: Non Filter Tips; Maxymum Recovery Pipette Tips - Axygen

Chicken (Normal/Adult) Universal (pool of tissue cDNA)

CHUC-565 10 rxn
EUR 498

Reference Solution (incl. in Reference electrode )

A11-240-1 1 unit Ask for price

Universal-1

OD308-1PK 1 unit
EUR 2.65
Description: Universal-1

Universal-1

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Description: Universal-1

Reference Housing

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Universal cDNA Reverse Transcribed by Random Hexamer: Dog Normal Tissues

C4734565-R 100 reactions
EUR 332

Universal cDNA Reverse Transcribed by Random Hexamer: Rat Normal Tissues

C4434567-R 100 reactions
EUR 332

Reference electrode

A11-140 1 unit
EUR 75

Reference electrode

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Universal cDNA Reverse Transcribed by Random Hexamer: Human Normal Tissues

C4234565-R 100 reactions
EUR 332

Universal cDNA Reverse Transcribed by Random Hexamer: Mouse Normal Tissues

C4334566-R 100 reactions
EUR 332

Universal cDNA Reverse Transcribed by Random Hexamer: Monkey Normal Tissues

C4534565-R 100 reactions
EUR 390

Universal cDNA Reverse Transcribed by Oligo dT Primer: Dog Normal Tissues

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EUR 332

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C4434567 100 reactions
EUR 332

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Comparative Genomics Identifies Genetic Locus in Plant-Associated Pseudomonas spp. It Is Necessary to Induced Systemic Vulnerabilities

Plant root-associated microbes promote plant growth and bring induced systemic resistance (ISR) to leaf pathogens. In an effort to find growth-promoting novel and ISR-inducing strains, we previously identified the root-associated strains of Pseudomonas spp. which promote plant growth but suddenly raises induction of systemic vulnerability (ISS) than the ISR to leaf pathogens.

Here, we show that the ISS-inducing phenotype is common among the root-associated Pseudomonas spp. Using comparative genomics, we identified a single locus of Pseudomonas fluorescens strain that is unique to ISS. We generated a net removal of the 11-gene loci ISS and found it necessary to phenotype ISS. Although the function of genes estimated in the locus that is not clear based on the similarity with genes of known function, the locus of the ISS is present in a variety of bacteria, and the part of the gene that was previously involved in the pathogenesis in animals. Collectively, these data suggest that a single locus contributes systemic bacterial plant modulation Microbiome immunity.

IMPORTANCE related bacteria can have various effects on the health of their hosts, but the genetic and molecular basis of these effects have largely remained elusive. This work indicates that the locus of new bacteria can regulate systemic plant immunity.

In addition, this work suggests that the growth-promoting strain may have unexpected consequences for the plant immunity, and this is very important to consider when microbiome plant is being engineered for agronomic improvements.

Total Protein - Multiple Sclerosis Disease: Brain: Cerebellum

P1236039Msc 1 mg
EUR 542

Total Protein - Multiple Sclerosis Disease: Brain: Frontal Lobe

P1236051Msc 1 mg
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Frozen Tissue Section - Multiple Sclerosis Disease: Brain: Pons

T1236071Msc 5 slides
EUR 523

Total Protein - Multiple Sclerosis Disease: Brain: Parietal Lobe

P1236066Msc 1 mg
EUR 542

Total Protein - Multiple Sclerosis Disease: Brain: Temporal Lobe

P1236078Msc 1 mg
EUR 542

Total Protein - Multiple Sclerosis Disease: Brain: Occipital Lobe

P1236062Msc 1 mg
EUR 542

Paraffin Tissue Section - Multiple Sclerosis Disease: Brain: Pons

T2236071Msc 5 slides
EUR 262

Total Protein - Multiple Sclerosis Disease: Brain: Corpus Callosum

P1236045Msc 1 mg
EUR 542

Total Protein - Multiple Sclerosis Disease: Brain: Medulla oblongata

P1236057Msc 1 mg
EUR 542

Total Protein - Multiple Sclerosis Disease: Brain: Postcentral Gyrus

P1236072Msc 1 mg
EUR 542

Total Protein - Multiple Sclerosis Disease: Brain: Precentral Gyrus

P1236073Msc 1 mg
EUR 542

Frozen Tissue Section - Multiple Sclerosis Disease: Brain: Amygdala

T1236036Msc 5 slides
EUR 523

Frozen Tissue Section - Multiple Sclerosis Disease: Brain: Pituitary

T1236068Msc 5 slides
EUR 971

Frozen Tissue Section - Multiple Sclerosis Disease: Brain: Thalamus

T1236079Msc 5 slides
EUR 523

Frozen Tissue Section - Multiple Sclerosis Disease: Brain: Cerebellum

T1236039Msc 5 slides
EUR 523

Frozen Tissue Section - Multiple Sclerosis Disease: Brain: Hippocampus

T1236052Msc 5 slides
EUR 971

Paraffin Tissue Section - Multiple Sclerosis Disease: Brain: Amygdala

T2236036Msc 5 slides
EUR 262

Paraffin Tissue Section - Multiple Sclerosis Disease: Brain: Thalamus

T2236079Msc 5 slides
EUR 262

Paraffin Tissue Section - Multiple Sclerosis Disease: Brain: Cerebellum

T2236039Msc 5 slides
EUR 262

Paraffin Tissue Section - Multiple Sclerosis Disease: Brain: Hippocampus

T2236052Msc 5 slides
EUR 834

Frozen Tissue Section - Multiple Sclerosis Disease: Brain: Frontal Lobe

T1236051Msc 5 slides
EUR 523

Genomic DNA - Multiple Sclerosis Disease: Brain, from a single donor

D1236035Msc 50 ug
EUR 562

Frozen Tissue Section - Multiple Sclerosis Disease: Brain: Occipital Lobe

T1236062Msc 5 slides
EUR 523

Frozen Tissue Section - Multiple Sclerosis Disease: Brain: Parietal Lobe

T1236066Msc 5 slides
EUR 523

Frozen Tissue Section - Multiple Sclerosis Disease: Brain: Temporal Lobe

T1236078Msc 5 slides
EUR 523

Paraffin Tissue Section - Multiple Sclerosis Disease: Brain: Frontal Lobe

T2236051Msc 5 slides
EUR 262

Frozen Tissue Section - Multiple Sclerosis Disease: Brain: Corpus Callosum

T1236045Msc 5 slides
EUR 523

Frozen Tissue Section - Multiple Sclerosis Disease: Brain: Precentral Gyrus

T1236073Msc 5 slides
EUR 523

Paraffin Tissue Section - Multiple Sclerosis Disease: Brain: Occipital Lobe

T2236062Msc 5 slides
EUR 262

Paraffin Tissue Section - Multiple Sclerosis Disease: Brain: Parietal Lobe

T2236066Msc 5 slides
EUR 262

Paraffin Tissue Section - Multiple Sclerosis Disease: Brain: Temporal Lobe

T2236078Msc 5 slides
EUR 262

Genomic DNA - Multiple Sclerosis Disease: Brain: Pons, from a single donor

D1236071Msc 50 ug
EUR 562

Frozen Tissue Section - Multiple Sclerosis Disease: Brain: Medulla oblongata

T1236057Msc 5 slides
EUR 523

Frozen Tissue Section - Multiple Sclerosis Disease: Brain: Postcentral Gyrus

T1236072Msc 5 slides
EUR 523

Paraffin Tissue Section - Multiple Sclerosis Disease: Brain: Corpus Callosum

T2236045Msc 5 slides
EUR 262

Paraffin Tissue Section - Multiple Sclerosis Disease: Brain: Precentral Gyrus

T2236073Msc 5 slides
EUR 262

Paraffin Tissue Section - Multiple Sclerosis Disease: Brain: Medulla oblongata

T2236057Msc 5 slides
EUR 262

Paraffin Tissue Section - Multiple Sclerosis Disease: Brain: Postcentral Gyrus

T2236072Msc 5 slides
EUR 262

Genomic DNA - Multiple Sclerosis Disease: Brain: Amygdala, from a single donor

D1236036Msc 50 ug
EUR 562

Genomic DNA - Multiple Sclerosis Disease: Brain: Thalamus, from a single donor

D1236079Msc 50 ug
EUR 562

Genomic DNA - Multiple Sclerosis Disease: Brain: Cerebellum, from a single donor

D1236039Msc 50 ug
EUR 562

Genomic DNA - Multiple Sclerosis Disease: Brain: Frontal Lobe, from a single donor

D1236051Msc 50 ug
EUR 562

Genomic DNA - Multiple Sclerosis Disease: Brain: Occipital Lobe, from a single donor

D1236062Msc 50 ug
EUR 562

Genomic DNA - Multiple Sclerosis Disease: Brain: Parietal Lobe, from a single donor

D1236066Msc 50 ug
EUR 562

Genomic DNA - Multiple Sclerosis Disease: Brain: Temporal Lobe, from a single donor

D1236078Msc 50 ug
EUR 562

Genomic DNA - Multiple Sclerosis Disease: Brain: Precentral Gyrus, from a single donor

D1236073Msc 50 ug
EUR 562

Genomic DNA - Multiple Sclerosis Disease: Brain: Medulla oblongata, from a single donor

D1236057MSC 50 ug
EUR 562

Genomic DNA - Multiple Sclerosis Disease: Brain: Postcentral Gyrus, from a single donor

D1236072Msc 50 ug
EUR 562

Human Multiple Sclerosis Leukopak

ABC-TC4294 1 pack Ask for price
Description: Multiple Sclerosis (MS) is a chronic disease that is often progressive. The disease is characterized by damage to the central nervous system. This includes the brain, spinal cord and the optic nerve. MS can cause numbness, severe fatigue, blurred vision and impaired speech and impaired muscular coordination.

Single Donor Human Multiple Sclerosis Serum

ISERSMSC each
EUR 209
Description: Single Donor Human Multiple Sclerosis Serum

Single Donor Human Multiple Sclerosis Plasma

IPLASMSC each
EUR 209
Description: Single Donor Human Multiple Sclerosis Plasma

Multiple types of colon disease with colon tissue array

CO245a each
EUR 66
Description: Multiple types of colon disease with colon tissue array, including crohn‘s disease, chronic inflammation of the colonic mucosa, ulcerative colitis, adenocarcinoma, 12 cases/24 cores (core size 1.5mm), replacing CO245

Multiple kidney disease with kidney tissue array (2016/ISUP WHO classification

KD804a each
EUR 258
Description: Multiple kidney disease with kidney tissue array (2016/ISUP WHO classification, Grade mean Isup Grade), including pathoogy grade, TNM and clinical stage (reference AJCC 8th version), 75 cases/80 cores, replacing KD804

Multiple diseases of liver tissue array

LV1201b each
EUR 474
Description: Multiple diseases of liver tissue array, includin pathology grade, TNM and clinical stage, 118 cases/120 cores, replacing LV1201a

Tsc1 (GFP-tagged) - Mouse tuberous sclerosis 1 (cDNA clone MGC:63425 IMAGE:5720967)

MG211709 10 µg Ask for price

Tsc1 (untagged) - Mouse tuberous sclerosis 1 (cDNA clone MGC:63425 IMAGE:5720967), (10ug)

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Multiple organ digestive system diseased tissue array with normal tissue as control

BCC000128 each
EUR 198
Description: Multiple organ digestive system diseased tissue array with normal tissue as control, including TNM, clinical stage and pathology grade, 40 cases/40 cores

Tsc1 (Myc-DDK-tagged) - Mouse tuberous sclerosis 1 (cDNA clone MGC:63425 IMAGE:5720967)

MR211709 10 µg Ask for price

Lenti ORF clone of Tsc1 (mGFP-tagged) - Mouse tuberous sclerosis 1 (cDNA clone MGC:63425 IMAGE:5720967)

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Tuberous Sclerosis 1 Antibody

20-abx116334
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Tuberous Sclerosis 1 Antibody

abx116334-50l 50 µl
EUR 612.5

Lenti ORF clone of Tsc1 (Myc-DDK-tagged) - Mouse tuberous sclerosis 1 (cDNA clone MGC:63425 IMAGE:5720967)

MR211709L3 10 µg Ask for price

Lenti ORF particles, Tsc1 (GFP-tagged) - Mouse tuberous sclerosis 1 (cDNA clone MGC:63425 IMAGE:5720967), 200ul, >10^7 TU/mL

MR211709L4V 200 µl Ask for price

Als2 (GFP-tagged) - Mouse amyotrophic lateral sclerosis 2 (juvenile) homolog (human) (cDNA clone MGC:27807 IMAGE:3257574)

MG211215 10 µg Ask for price

cDNA - Alzheimer's Disease: Brain

C1236035Alz 40 reactions
EUR 802

cDNA - Parkinson's Disease: Brain

C1236035Par 40 reactions
EUR 802

Lenti ORF particles, Tsc1 (Myc-DDK-tagged) - Mouse tuberous sclerosis 1 (cDNA clone MGC:63425 IMAGE:5720967), 200ul, >10^7 TU/mL

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Tuberous Sclerosis Protein 2 (TSC2) Antibody

20-abx127069
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Tuberous Sclerosis Protein 2 (TSC2) Antibody

20-abx134972
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Tuberous Sclerosis Protein 2 (TSC2) Antibody

abx031372-400ul 400 ul
EUR 627.6

Tuberous Sclerosis Protein 2 (TSC2) Antibody

abx031372-80l 80 µl
EUR 343.2

Tuberous Sclerosis Protein 2 (TSC2) Antibody

abx034676-400ul 400 ul
EUR 627.6

Tuberous Sclerosis Protein 2 (TSC2) Antibody

abx034676-80l 80 µl
EUR 343.2

Tuberous Sclerosis Protein 2 (TSC2) Antibody

abx025288-400ul 400 ul
EUR 627.6

Tuberous Sclerosis Protein 2 (TSC2) Antibody

abx025288-80l 80 µl
EUR 343.2

Tuberous Sclerosis Protein 2 (TSC2) Antibody

20-abx213738
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Tuberous Sclerosis Protein 2 (TSC2) Antibody

20-abx213927
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Tuberous Sclerosis Protein 2 (TSC2) Antibody

20-abx213945
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Tuberous Sclerosis Protein 2 (TSC2) Antibody

20-abx326673
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Tuberous Sclerosis Protein 2 (TSC2) Antibody

20-abx328567
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  • 100 ug
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Tuberous Sclerosis Protein 2 (TSC2) Antibody

20-abx329216
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