Comparative genomics and pangenome-oriented studies reveal high homogeneity

Comparative genomics and pangenome-oriented studies reveal high homogeneity of the agronomically relevant enterobacterial plant pathogen Dickeya solani

 

Comparative genomics and pangenome-oriented studies reveal high homogeneity

Background: Dickeya solani is an important plant pathogenic bacteria that caused heavy losses in the production of potatoes in Europe. This species attracted much attention because of the virulence of extraordinary potential to destroy the large and spread more easily contrasted with other spp Dickeya. Given the high need for extensive studies on important economic soft rot Pectobacteriaceae, we perform comparative genomics analysis on the strain D. solani to search for the genetic foundation that would explain the difference in the level of virulence observed in D. solani population.


Results: High quality assemblies from 8 de novo genome sequencing D. solani has been obtained. Whole-sequence comparison, Anib, anim, Tetra and oriented pangenome analysis performed at the genome and sequences of 14 additional strains revealed a very high degree of homogeneity among the studied genetic material D. solani strains. By using 22 genomes, pangenome D. solani, which consists of a core of 84.7%, 7.2% and genes unique accessory 8.1%, has been almost completely determined, indicates the structure pangenome almost closed.

Attribution of genes included in D. solani pangenome fraction of COG functional categories shows that a higher percentage of unique accessories and pangenome different parts of the core part encountered in phage / mobile elements and transcription- groups associated with RNS genome 05.1.2A yeast strains have the most significant impact. Also, D. solani first large-scale genome-wide gene phylogeny calculated alignments core of this serial here reported.


Conclusion: The status almost closed D. solani pangenome achieved in this work points to the fact that the unique genes from these species should no longer growing. these features are characteristic of a good representative taxa occupy isolated ecological niches or lack of efficient mechanisms to exchange genes and recombination, which seems rational about strict pathogen species with clonal population structure. Finally, there is no clear correlation between the geographic origin of strains of D. solani and phylogeny they were found, which may reflect the peculiarities of the international potato seed market.

 Comparative genomics and pangenome-oriented studies reveal high homogeneity of the agronomically relevant enterobacterial plant pathogen Dickeya solani
Comparative genomics and pangenome-oriented studies reveal high homogeneity of the agronomically relevant enterobacterial plant pathogen Dickeya solani

Pig Universal Reference cDNA-Oligo dT

PD-UR-80 2x40 reactions
EUR 461

Rat Universal Reference cDNA-Oligo dT

RD-UR-40 1x40 reactions
EUR 243

Rat Universal Reference cDNA-Oligo dT

RD-UR-80 2x40 reactions
EUR 461

Human Universal Reference cDNA-Oligo dT

HD-UR-40 1x40 reactions
EUR 280

Human Universal Reference cDNA-Oligo dT

HD-UR-80 2x40 reactions
EUR 498

Mouse Universal Reference cDNA-Oligo dT

MD-UR-40 1x40 reactions
EUR 243

Mouse Universal Reference cDNA-Oligo dT

MD-UR-80 2x40 reactions
EUR 461

Sheep Universal Reference cDNA-Oligo dT

SD-UR-40 1x40 reactions
EUR 243

Sheep Universal Reference cDNA-Oligo dT

SD-UR-80 2x40 reactions
EUR 461

Monkey Universal Reference cDNA-Oligo dT

KD-UR-40 1x40 reactions
EUR 280

Monkey Universal Reference cDNA-Oligo dT

KD-UR-80 2x40 reactions
EUR 498

Chicken Universal Reference cDNA-Oligo dT

CD-UR-40 1x40 reactions
EUR 243

Chicken Universal Reference cDNA-Oligo dT

CD-UR-80 2x40 reactions
EUR 461

Dog Universal Reference cDNA-Random Hexamer

DD-UR-40-RH 1x40 reactions
EUR 280

Dog Universal Reference cDNA-Random Hexamer

DD-UR-80-RH 2x40 reactions
EUR 498

Pig Universal Reference cDNA-Random Hexamer

PD-UR-40-RH 1x40 reactions
EUR 243

Pig Universal Reference cDNA-Random Hexamer

PD-UR-80-RH 2x40 reactions
EUR 461

Rat Universal Reference cDNA-Random Hexamer

RD-UR-40-RH 1x40 reactions
EUR 243

Rat Universal Reference cDNA-Random Hexamer

RD-UR-80-RH 2x40 reactions
EUR 461

Human Universal Reference cDNA-Random Hexamer

HD-UR-40-RH 1x40 reactions
EUR 280

Human Universal Reference cDNA-Random Hexamer

HD-UR-80-RH 2x40 reactions
EUR 498

Mouse Universal Reference cDNA-Random Hexamer

MD-UR-40-RH 1x40 reactions
EUR 243

Mouse Universal Reference cDNA-Random Hexamer

MD-UR-80-RH 2x40 reactions
EUR 461

Sheep Universal Reference cDNA-Random Hexamer

SD-UR-40-RH 1x40 reactions
EUR 243

Sheep Universal Reference cDNA-Random Hexamer

SD-UR-80-RH 2x40 reactions
EUR 461

Monkey Universal Reference cDNA-Random Hexamer

KD-UR-40-RH 1x40 reactions
EUR 280

Monkey Universal Reference cDNA-Random Hexamer

KD-UR-80-RH 2x40 reactions
EUR 498

Chicken Universal Reference cDNA-Random Hexamer

CD-UR-40-RH 1x40 reactions
EUR 243

Chicken Universal Reference cDNA-Random Hexamer

CD-UR-80-RH 2x40 reactions
EUR 461

Dog Universal Reference Total RNA

DR-UR-100 1x0.1mg
EUR 187

Dog Universal Reference Total RNA

DR-UR-200 2x0.1mg
EUR 358

Dog Universal Reference Total RNA

DR-UR-400 4x0.1mg
EUR 582

Pig Universal Reference Total RNA

PR-UR-100 1x0.1mg
EUR 187

Pig Universal Reference Total RNA

PR-UR-200 2x0.1mg
EUR 358

Pig Universal Reference Total RNA

PR-UR-400 4x0.1mg
EUR 582

Rat Universal Reference Total RNA

RR-UR-100 1x0.1mg
EUR 187

Rat Universal Reference Total RNA

RR-UR-200 2x0.1mg
EUR 358

Rat Universal Reference Total RNA

RR-UR-400 4x0.1mg
EUR 582

Human Universal Reference Total RNA

HR-UR-50 1x0.05mg
EUR 187

Mouse Universal Reference Total RNA

MR-UR-100 1x0.1mg
EUR 187

Mouse Universal Reference Total RNA

MR-UR-200 2x0.1mg
EUR 358

Mouse Universal Reference Total RNA

MR-UR-400 4x0.1mg
EUR 582

Sheep Universal Reference Total RNA

SR-UR-100 1x0.1mg
EUR 187

Sheep Universal Reference Totol RNA

SR-UR-200 2x0.1mg
EUR 358

Sheep Universal Reference Total RNA

SR-UR-400 4x0.1mg
EUR 582

Monkey Universal Reference Total RNA

KR-UR-100 1x0.1mg
EUR 187

Monkey Universal Reference Total RNA

KR-UR-200 2x0.1mg
EUR 358

Monkey Universal Reference Total RNA

KR-UR-400 4x0.1mg
EUR 582

Chicken Universal Reference Total RNA

CR-UR-100 1x0.1mg
EUR 187

Chicken Universal Reference Total RNA

CR-UR-200 2x0.1mg
EUR 358

Chicken Universal Reference Total RNA

CR-UR-400 4x0.1mg
EUR 582

200UL "MAXYMUM RECOVERY" UNIVERSAL FIT REFERENCE TIPS

TR-222-C-L 1000/pk
EUR 297.6
Description: Non Filter Tips; Maxymum Recovery Pipette Tips - Axygen

200UL "MAXYMUM RECOVERY" UNIVERSAL FIT REFERENCE TIPS, RACKED

TR-222-C-L-R 960/pk
EUR 187.2
Description: Non Filter Tips; Maxymum Recovery Pipette Tips - Axygen

200UL YELLOW, STERILIZED UNIVERSAL FIT PIPET TIPS WITH REFERENCE LINES

TR-222-Y-R-S 960/pk
EUR 168
Description: Non Filter Tips; Pipette Tips - Axygen

200UL CLEAR MAXYMUM RECOVERY UNIVERSAL FIT PIPET TIPS WITH REFERENCE LINES

TR-222-C-L-STK 480/pk
EUR 162
Description: Non Filter Tips; Maxymum Recovery Pipette Tips - Axygen

200UL "MAXYMUM RECOVERY" UNIVERSAL FIT REFERENCE TIPS, RACKED AND PRE-STERILIZED

TR-222-C-L-R-S 960/pk
EUR 230.4
Description: Non Filter Tips; Maxymum Recovery Pipette Tips - Axygen

200UL CLEAR, MAXYMUM RECOVERY STERILE UNIVERSAL FIT PIPET TIPS WITH REFERENCE LINES

TR-222-C-STK-S 480/pk
EUR 158.4
Description: Non Filter Tips; Pipette Tips - Axygen

200UL CLEAR PRE-STERILIZED MAXYMUM RECOVERY UNIVERSAL FIT PIPET TIPS WITH REFERENCE LINES

TR-222-C-L-STK-S 480/pk
EUR 184.8
Description: Non Filter Tips; Maxymum Recovery Pipette Tips - Axygen

Reference Solution (incl. in Reference electrode )

A11-240-1 1 unit Ask for price

Chicken (Normal/Adult) Universal (pool of tissue cDNA)

CHUC-565 10 rxn
EUR 498

Reference Housing

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EUR 155

Reference electrode

A11-140 1 unit
EUR 75

Reference electrode

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ROX Reference Dye

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Description: ROX Reference Dye

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Description: ROX Reference Dye

ROX Reference Dye

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Description: ROX Reference Dye

ROX Reference Dye

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EUR 115

ROX Reference Dye

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ROX Reference Dye

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EUR 190

ROX Reference Dye

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EUR 255

ROX Reference Dye

MBS8579842-5x005mL 5x0.05mL
EUR 465

ROX Reference Dye *50X fluorescence reference solution for PCR reactions*

400 5 mL
EUR 227
Description: ROX Reference Dye is equivalent to 12223-012 (Life Technology).

ROX Reference Dye *50X fluorescence reference solution for PCR reactions*

400-5mL 5 mL
EUR 222
Description: ROX Reference Dye is equivalent to 12223-012 (Life Technology).

ISE Reference Housing

A11-400-1 1 unit Ask for price

RPE REFERENCE STANDARD

MBS238353-100Tests 100Tests
EUR 715

RPE REFERENCE STANDARD

MBS238353-5x100Tests 5x100Tests
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50×ROX Reference Dye

EQ012-1ml 1ml
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50×ROX Reference Dye

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50×ROX Reference Dye

EQ012 500ul
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DAPI Reference Standard

BLI906A-1 1ml
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Comparative Genomics Identifies Genetic Locus in Plant-Associated Pseudomonas spp. It Is Necessary to Induced Systemic Vulnerabilities

Plant root-associated microbes promote plant growth and bring induced systemic resistance (ISR) to leaf pathogens. In an effort to find growth-promoting novel and ISR-inducing strains, we previously identified the root-associated strains of Pseudomonas spp. which promote plant growth but suddenly raises induction of systemic vulnerability (ISS) than the ISR to leaf pathogens.

Here, we show that the ISS-inducing phenotype is common among the root-associated Pseudomonas spp. Using comparative genomics, we identified a single locus of Pseudomonas fluorescens strain that is unique to ISS. We generated a net removal of the 11-gene loci ISS and found it necessary to phenotype ISS. Although the function of genes estimated in the locus that is not clear based on the similarity with genes of known function, the locus of the ISS is present in a variety of bacteria, and the part of the gene that was previously involved in the pathogenesis in animals. Collectively, these data suggest that a single locus contributes systemic bacterial plant modulation Microbiome immunity.

IMPORTANCE related bacteria can have various effects on the health of their hosts, but the genetic and molecular basis of these effects have largely remained elusive. This work indicates that the locus of new bacteria can regulate systemic plant immunity.

In addition, this work suggests that the growth-promoting strain may have unexpected consequences for the plant immunity, and this is very important to consider when microbiome plant is being engineered for agronomic improvements.

Total Protein - Multiple Sclerosis Disease: Brain: Cerebellum

P1236039Msc 1 mg
EUR 542

Total Protein - Multiple Sclerosis Disease: Brain: Frontal Lobe

P1236051Msc 1 mg
EUR 542

Frozen Tissue Section - Multiple Sclerosis Disease: Brain: Pons

T1236071Msc 5 slides
EUR 523

Total Protein - Multiple Sclerosis Disease: Brain: Parietal Lobe

P1236066Msc 1 mg
EUR 542

Total Protein - Multiple Sclerosis Disease: Brain: Temporal Lobe

P1236078Msc 1 mg
EUR 542

Total Protein - Multiple Sclerosis Disease: Brain: Occipital Lobe

P1236062Msc 1 mg
EUR 542

Paraffin Tissue Section - Multiple Sclerosis Disease: Brain: Pons

T2236071Msc 5 slides
EUR 262

Tissue, Genomic DNA, Human Disease (Adult), Multiple Sclerosis Disease, Brain, BioGenomics

MBS654459-005mg 0.05mg
EUR 715

Tissue, Genomic DNA, Human Disease (Adult), Multiple Sclerosis Disease, Brain, BioGenomics

MBS654459-5x005mg 5x0.05mg
EUR 3075

Total Protein - Multiple Sclerosis Disease: Brain: Corpus Callosum

P1236045Msc 1 mg
EUR 542

Total Protein - Multiple Sclerosis Disease: Brain: Medulla oblongata

P1236057Msc 1 mg
EUR 542

Total Protein - Multiple Sclerosis Disease: Brain: Postcentral Gyrus

P1236072Msc 1 mg
EUR 542

Total Protein - Multiple Sclerosis Disease: Brain: Precentral Gyrus

P1236073Msc 1 mg
EUR 542

Frozen Tissue Section - Multiple Sclerosis Disease: Brain: Amygdala

T1236036Msc 5 slides
EUR 523

Frozen Tissue Section - Multiple Sclerosis Disease: Brain: Pituitary

T1236068Msc 5 slides
EUR 971

Frozen Tissue Section - Multiple Sclerosis Disease: Brain: Thalamus

T1236079Msc 5 slides
EUR 523

Paraffin Tissue Section - Multiple Sclerosis Disease: Brain: Amygdala

T2236036Msc 5 slides
EUR 262

Paraffin Tissue Section - Multiple Sclerosis Disease: Brain: Thalamus

T2236079Msc 5 slides
EUR 262

Frozen Tissue Section - Multiple Sclerosis Disease: Brain: Cerebellum

T1236039Msc 5 slides
EUR 523

Frozen Tissue Section - Multiple Sclerosis Disease: Brain: Hippocampus

T1236052Msc 5 slides
EUR 971

Tissue, Total Protein, Human Disease, Multiple Sclerosis, Brain

MBS657433-1mg 1mg
EUR 820

Tissue, Total Protein, Human Disease, Multiple Sclerosis, Brain

MBS657433-5x1mg 5x1mg
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Paraffin Tissue Section - Multiple Sclerosis Disease: Brain: Cerebellum

T2236039Msc 5 slides
EUR 262

Paraffin Tissue Section - Multiple Sclerosis Disease: Brain: Hippocampus

T2236052Msc 5 slides
EUR 834

Tissue, Genomic DNA, Human Disease (Adult), Multiple Sclerosis Disease, Brain, Pons, BioGenomics

MBS654324-005mg 0.05mg
EUR 715

Tissue, Genomic DNA, Human Disease (Adult), Multiple Sclerosis Disease, Brain, Pons, BioGenomics

MBS654324-5x005mg 5x0.05mg
EUR 3075

Tissue, Section, Human Disease, Multiple Sclerosis, Brain (Frozen)

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EUR 755

Tissue, Section, Human Disease, Multiple Sclerosis, Brain (Frozen)

MBS639775-5x5Slides 5x5Slides
EUR 3170

Frozen Tissue Section - Multiple Sclerosis Disease: Brain: Frontal Lobe

T1236051Msc 5 slides
EUR 523

Genomic DNA - Multiple Sclerosis Disease: Brain, from a single donor

D1236035Msc 50 ug
EUR 562

Tissue, Section, Human Disease, Multiple Sclerosis, Brain (Paraffin)

MBS640003-5Sections 5Sections
EUR 510

Tissue, Section, Human Disease, Multiple Sclerosis, Brain (Paraffin)

MBS640003-5x5Sections 5x5Sections
EUR 2145

Paraffin Tissue Section - Multiple Sclerosis Disease: Brain: Frontal Lobe

T2236051Msc 5 slides
EUR 262

Frozen Tissue Section - Multiple Sclerosis Disease: Brain: Occipital Lobe

T1236062Msc 5 slides
EUR 523

Frozen Tissue Section - Multiple Sclerosis Disease: Brain: Parietal Lobe

T1236066Msc 5 slides
EUR 523

Frozen Tissue Section - Multiple Sclerosis Disease: Brain: Temporal Lobe

T1236078Msc 5 slides
EUR 523

Tissue, Genomic DNA, Human Disease (Adult), Multiple Sclerosis Disease, Brain, Thalamus, BioGenomics

MBS654278-005mg 0.05mg
EUR 715

Tissue, Genomic DNA, Human Disease (Adult), Multiple Sclerosis Disease, Brain, Thalamus, BioGenomics

MBS654278-5x005mg 5x0.05mg
EUR 3075

Tissue, Genomic DNA, Human Disease (Adult), Multiple Sclerosis Disease, Brain, Amygdala, BioGenomics

MBS654350-005mg 0.05mg
EUR 715

Tissue, Genomic DNA, Human Disease (Adult), Multiple Sclerosis Disease, Brain, Amygdala, BioGenomics

MBS654350-5x005mg 5x0.05mg
EUR 3075

Tissue, Genomic DNA, Human Disease (Adult), Multiple Sclerosis Disease, Brain, Cerebellum, BioGenomics

MBS654439-005mg 0.05mg
EUR 715

Tissue, Genomic DNA, Human Disease (Adult), Multiple Sclerosis Disease, Brain, Cerebellum, BioGenomics

MBS654439-5x005mg 5x0.05mg
EUR 3075

Paraffin Tissue Section - Multiple Sclerosis Disease: Brain: Occipital Lobe

T2236062Msc 5 slides
EUR 262

Paraffin Tissue Section - Multiple Sclerosis Disease: Brain: Parietal Lobe

T2236066Msc 5 slides
EUR 262

Paraffin Tissue Section - Multiple Sclerosis Disease: Brain: Temporal Lobe

T2236078Msc 5 slides
EUR 262

Frozen Tissue Section - Multiple Sclerosis Disease: Brain: Corpus Callosum

T1236045Msc 5 slides
EUR 523

Frozen Tissue Section - Multiple Sclerosis Disease: Brain: Precentral Gyrus

T1236073Msc 5 slides
EUR 523

Tissue, Total Protein, Human Disease, Multiple Sclerosis, Brain, Pons

MBS657319-1mg 1mg
EUR 820

Tissue, Total Protein, Human Disease, Multiple Sclerosis, Brain, Pons

MBS657319-5x1mg 5x1mg
EUR 3470

Paraffin Tissue Section - Multiple Sclerosis Disease: Brain: Corpus Callosum

T2236045Msc 5 slides
EUR 262

Paraffin Tissue Section - Multiple Sclerosis Disease: Brain: Precentral Gyrus

T2236073Msc 5 slides
EUR 262

Frozen Tissue Section - Multiple Sclerosis Disease: Brain: Medulla oblongata

T1236057Msc 5 slides
EUR 523

Frozen Tissue Section - Multiple Sclerosis Disease: Brain: Postcentral Gyrus

T1236072Msc 5 slides
EUR 523

Tissue, Genomic DNA, Human Disease (Adult), Multiple Sclerosis Disease, Brain, Parietal Lobe, BioGenomics

MBS654499-005mg 0.05mg
EUR 715

Tissue, Genomic DNA, Human Disease (Adult), Multiple Sclerosis Disease, Brain, Parietal Lobe, BioGenomics

MBS654499-5x005mg 5x0.05mg
EUR 3075

Tissue, Genomic DNA, Human Disease (Adult), Multiple Sclerosis Disease, Brain, Frontal Lobe, BioGenomics

MBS654543-005mg 0.05mg
EUR 715

Tissue, Genomic DNA, Human Disease (Adult), Multiple Sclerosis Disease, Brain, Frontal Lobe, BioGenomics

MBS654543-5x005mg 5x0.05mg
EUR 3075

Tissue, Genomic DNA, Human Disease (Adult), Multiple Sclerosis Disease, Brain, Temporal Lobe, BioGenomics

MBS654555-005mg 0.05mg
EUR 715

Tissue, Genomic DNA, Human Disease (Adult), Multiple Sclerosis Disease, Brain, Temporal Lobe, BioGenomics

MBS654555-5x005mg 5x0.05mg
EUR 3075

Genomic DNA - Multiple Sclerosis Disease: Brain: Pons, from a single donor

D1236071Msc 50 ug
EUR 562

Tissue, Section, Human Disease, Multiple Sclerosis, Brain, Pons (Frozen)

MBS640012-5Slides 5Slides
EUR 755

Tissue, Section, Human Disease, Multiple Sclerosis, Brain, Pons (Frozen)

MBS640012-5x5Slides 5x5Slides
EUR 3170

Paraffin Tissue Section - Multiple Sclerosis Disease: Brain: Medulla oblongata

T2236057Msc 5 slides
EUR 262

Paraffin Tissue Section - Multiple Sclerosis Disease: Brain: Postcentral Gyrus

T2236072Msc 5 slides
EUR 262

Tissue, Section, Human Disease, Multiple Sclerosis, Brain, Pons (Paraffin)

MBS640884-5Slides 5Slides
EUR 510

Tissue, Section, Human Disease, Multiple Sclerosis, Brain, Pons (Paraffin)

MBS640884-5x5Slides 5x5Slides
EUR 2145

Tissue, Genomic DNA, Human Disease (Adult), Multiple Sclerosis Disease, Brain, Occipital Lobe, BioGenomics

MBS654608-005mg 0.05mg
EUR 715

Tissue, Genomic DNA, Human Disease (Adult), Multiple Sclerosis Disease, Brain, Occipital Lobe, BioGenomics

MBS654608-5x005mg 5x0.05mg
EUR 3075

Tissue, Genomic DNA, Human Disease (Adult), Multiple Sclerosis Disease, Brain, Medulla Oblongata, BioGenomics

MBS654616-005mg 0.05mg
EUR 715

Tissue, Genomic DNA, Human Disease (Adult), Multiple Sclerosis Disease, Brain, Medulla Oblongata, BioGenomics

MBS654616-5x005mg 5x0.05mg
EUR 3075

Tissue, Genomic DNA, Human Disease (Adult), Multiple Sclerosis Disease, Brain, Postcentral Gyrus, BioGenomics

MBS654431-005mg 0.05mg
EUR 715

Tissue, Genomic DNA, Human Disease (Adult), Multiple Sclerosis Disease, Brain, Postcentral Gyrus, BioGenomics

MBS654431-5x005mg 5x0.05mg
EUR 3075

Tissue, Genomic DNA, Human Disease (Adult), Multiple Sclerosis Disease, Brain, Precentral Gyrus, BioGenomics

MBS654586-005mg 0.05mg
EUR 715

Tissue, Genomic DNA, Human Disease (Adult), Multiple Sclerosis Disease, Brain, Precentral Gyrus, BioGenomics

MBS654586-5x005mg 5x0.05mg
EUR 3075

Tissue, Section, Human Disease, Multiple Sclerosis, Brain, Amygdala (Frozen)

MBS640618-5Slides 5Slides
EUR 755

Tissue, Section, Human Disease, Multiple Sclerosis, Brain, Amygdala (Frozen)

MBS640618-5x5Slides 5x5Slides
EUR 3170

Tissue, Section, Human Disease, Multiple Sclerosis, Brain, Thalamus (Frozen)

MBS640264-5Slides 5Slides
EUR 755

Tissue, Section, Human Disease, Multiple Sclerosis, Brain, Thalamus (Frozen)

MBS640264-5x5Slides 5x5Slides
EUR 3170

Tissue, Total Protein, Human Disease, Multiple Sclerosis, Brain, Thalamus

MBS657134-1mg 1mg
EUR 820

Tissue, Total Protein, Human Disease, Multiple Sclerosis, Brain, Thalamus

MBS657134-5x1mg 5x1mg
EUR 3470

Tissue, Total Protein, Human Disease, Multiple Sclerosis, Brain, Amygdala

MBS657349-1mg 1mg
EUR 820

 

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